ID T2IUC4_CROWT Unreviewed; 294 AA.
AC T2IUC4;
DT 13-NOV-2013, integrated into UniProtKB/TrEMBL.
DT 13-NOV-2013, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=CWATWH0005_5792 {ECO:0000313|EMBL:CCQ56514.1};
OS Crocosphaera watsonii WH 0005.
OC Bacteria; Cyanobacteriota; Cyanophyceae; Oscillatoriophycideae;
OC Chroococcales; Aphanothecaceae; Crocosphaera.
OX NCBI_TaxID=423472 {ECO:0000313|EMBL:CCQ56514.1, ECO:0000313|Proteomes:UP000017981};
RN [1] {ECO:0000313|EMBL:CCQ56514.1, ECO:0000313|Proteomes:UP000017981}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WH 0005 {ECO:0000313|EMBL:CCQ56514.1,
RC ECO:0000313|Proteomes:UP000017981};
RA Bench S.;
RL Submitted (JAN-2013) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:CCQ56514.1, ECO:0000313|Proteomes:UP000017981}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WH 0005 {ECO:0000313|EMBL:CCQ56514.1,
RC ECO:0000313|Proteomes:UP000017981};
RA Bench S.R., Heller P., Frank I., Arciniega M., Shilova I.N., Zehr J.P.;
RT "Whole genome comparison of six Crocosphaera watsonii strains with
RT differing phenotypes.";
RL Submitted (SEP-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCQ56514.1}.
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DR EMBL; CAQL01000640; CCQ56514.1; -; Genomic_DNA.
DR AlphaFoldDB; T2IUC4; -.
DR Proteomes; UP000017981; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR PANTHER; PTHR42933:SF3; TYPE I RESTRICTION ENZYME MJAVIII METHYLASE SUBUNIT; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:CCQ56514.1};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:CCQ56514.1}.
FT DOMAIN 15..259
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
FT COILED 256..290
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 294 AA; 33457 MW; 123FD312B14B644A CRC64;
MKQEINIKVA QEIPDHNYAL FGQEVNGSTW ALARMNMFLH GEDGARIDWS DTLNSPTLTD
NTTNLQKFDV VVANPPFSLD KWGAENAKND VFNRFWRDIP PKTKVDYGFI THMIEVALPK
QGRVVVIAPH GVLFRGSSEG RIGKKLIEEN LLDAVIGLPP NLFPSTSIPV AVLIFDRSRE
EGGEREACQE VMFIDASSDF QSGKNQNLLL DSHLEKIVKT YLNWQDVDKY AHVATFEEIE
ENDFNLNIPR YVDTFEEEEE IDLKEVQAEI ESLEDQLGEV RGKMRDYLKE LGVL
//
