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Database: UniProt
Entry: TPO_MOUSE
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ID   TPO_MOUSE               Reviewed;         356 AA.
AC   P40226;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   19-MAR-2014, entry version 106.
DE   RecName: Full=Thrombopoietin;
DE   AltName: Full=C-mpl ligand;
DE            Short=ML;
DE   AltName: Full=Megakaryocyte colony-stimulating factor;
DE   AltName: Full=Megakaryocyte growth and development factor;
DE            Short=MGDF;
DE   AltName: Full=Myeloproliferative leukemia virus oncogene ligand;
DE   Flags: Precursor;
GN   Name=Thpo;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC   TISSUE=Kidney;
RX   PubMed=8202158; DOI=10.1038/369565a0;
RA   Lok S., Kaushansky K., Holly R.D., Kuijper J.L., Lofton-Day C.E.,
RA   Oort P.J., Grant F.J., Heipel M.D., Burkhead S.K., Kramer J.M.,
RA   Bell L.A.N., Sprecher C.A., Blumberg H., Johnson R., Prunkard D.,
RA   Ching A.F.T., Mathewes S.L., Bailey M.C., Forstrom J.W., Buddle M.M.,
RA   Osborn S.G., Evans S.J., Sheppard P.O., Presnell S.R., O'Hara P.J.,
RA   Hagen F.S., Roth G.J., Foster D.C.;
RT   "Cloning and expression of murine thrombopoietin cDNA and stimulation
RT   of platelet production in vivo.";
RL   Nature 369:565-568(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC   TISSUE=Liver;
RX   PubMed=8020099; DOI=10.1016/0092-8674(94)90450-2;
RA   Bartley T.D., Bogenberger J., Hunt P., Li Y.-S., Lu H.S., Martin F.,
RA   Chang M.-S., Samal B.B., Nichol J.L., Swift S., Johnson M.J.,
RA   Hsu R.-Y., Parker V.P., Suggs S., Skrine J.D., Merewether L.A.,
RA   Clogson C., Hsu E., Hokom M.M., Hornkohl A., Choi E., Pangelinan M.,
RA   Sun Y., Mar V., McNich J., Simonet L., Jacobsen F., Xie C.,
RA   Shutter J., Chute H., Basu R., Selander L., Trollinger D., Sieu L.,
RA   Padilla D., Trail G., Elliott G., Izumi R., Covey T., Crouse J.,
RA   Garcia A., Xu W., del Castillo J., Biron J., Cole S., Hu M.C.-T.,
RA   Pacifici R., Ponting I., Saris C., Wen D., Yung Y.P., Lin H.,
RA   Bosselman R.A.;
RT   "Identification and cloning of a megakaryocyte growth and development
RT   factor that is a ligand for the cytokine receptor Mpl.";
RL   Cell 77:1117-1124(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RX   PubMed=7849319;
RA   Gurney A.L., Kuang W.-J., Xie M.-H., Malloy B.E., Eaton D.L.,
RA   de Sauvage F.J.;
RT   "Genomic structure, chromosomal localization, and conserved
RT   alternative splice forms of thrombopoietin.";
RL   Blood 85:981-988(1995).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   STRAIN=FVB/N; TISSUE=Mammary gland;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Lineage-specific cytokine affecting the proliferation
CC       and maturation of megakaryocytes from their committed progenitor
CC       cells. It acts at a late stage of megakaryocyte development. It
CC       may be the major physiological regulator of circulating platelets.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=P40226-1; Sequence=Displayed;
CC       Name=2; Synonyms=TPO-2;
CC         IsoId=P40226-2; Sequence=VSP_001452;
CC   -!- TISSUE SPECIFICITY: Found mainly in the liver, kidney and skeletal
CC       muscle.
CC   -!- DOMAIN: Two-domain structure with an erythropoietin-like N-
CC       terminal and a Ser/Pro/Thr-rich C-terminal.
CC   -!- SIMILARITY: Belongs to the EPO/TPO family.
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DR   EMBL; L34169; AAA40436.1; -; mRNA.
DR   EMBL; BC003803; AAH03803.1; -; mRNA.
DR   PIR; S45330; S45330.
DR   RefSeq; NP_001166976.1; NM_001173505.1.
DR   RefSeq; NP_033405.1; NM_009379.3.
DR   UniGene; Mm.3943; -.
DR   ProteinModelPortal; P40226; -.
DR   SMR; P40226; 28-172.
DR   STRING; 10090.ENSMUSP00000075756; -.
DR   PhosphoSite; P40226; -.
DR   PRIDE; P40226; -.
DR   Ensembl; ENSMUST00000076422; ENSMUSP00000075756; ENSMUSG00000022847. [P40226-1]
DR   Ensembl; ENSMUST00000115437; ENSMUSP00000111097; ENSMUSG00000022847. [P40226-1]
DR   GeneID; 21832; -.
DR   KEGG; mmu:21832; -.
DR   UCSC; uc007yra.2; mouse. [P40226-1]
DR   CTD; 7066; -.
DR   MGI; MGI:101875; Thpo.
DR   eggNOG; NOG84342; -.
DR   HOGENOM; HOG000082495; -.
DR   HOVERGEN; HBG002468; -.
DR   InParanoid; P40226; -.
DR   KO; K06854; -.
DR   OMA; EQSKAQD; -.
DR   OrthoDB; EOG7PVWPW; -.
DR   TreeFam; TF338084; -.
DR   NextBio; 301278; -.
DR   PRO; PR:P40226; -.
DR   ArrayExpress; P40226; -.
DR   Bgee; P40226; -.
DR   CleanEx; MM_THPO; -.
DR   Genevestigator; P40226; -.
DR   GO; GO:0005615; C:extracellular space; IEA:UniProtKB-KW.
DR   GO; GO:0008283; P:cell proliferation; IEA:InterPro.
DR   GO; GO:0030099; P:myeloid cell differentiation; IDA:MGI.
DR   GO; GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; IDA:UniProtKB.
DR   GO; GO:0045654; P:positive regulation of megakaryocyte differentiation; IDA:UniProtKB.
DR   GO; GO:0051897; P:positive regulation of protein kinase B signaling; IDA:UniProtKB.
DR   GO; GO:0001934; P:positive regulation of protein phosphorylation; IDA:UniProtKB.
DR   GO; GO:0038163; P:thrombopoietin-mediated signaling pathway; IDA:UniProtKB.
DR   Gene3D; 1.20.1250.10; -; 1.
DR   InterPro; IPR009079; 4_helix_cytokine-like_core.
DR   InterPro; IPR012351; 4_helix_cytokine_core.
DR   InterPro; IPR019767; EPO/TPO_CS.
DR   InterPro; IPR001323; EPO_TPO.
DR   InterPro; IPR003978; Thrombopoeitin.
DR   PANTHER; PTHR10560; PTHR10560; 1.
DR   Pfam; PF00758; EPO_TPO; 1.
DR   PRINTS; PR01485; THROMBOPTN.
DR   SUPFAM; SSF47266; SSF47266; 1.
DR   PROSITE; PS00817; EPO_TPO; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Complete proteome; Cytokine; Disulfide bond;
KW   Glycoprotein; Hormone; Reference proteome; Secreted; Signal.
FT   SIGNAL        1     21       Potential.
FT   CHAIN        22    356       Thrombopoietin.
FT                                /FTId=PRO_0000008412.
FT   CARBOHYD    197    197       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    206    206       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    235    235       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    249    249       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    256    256       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    336    336       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    351    351       N-linked (GlcNAc...) (Potential).
FT   DISULFID     28    172       Potential.
FT   DISULFID     50    106       Potential.
FT   VAR_SEQ     133    136       Missing (in isoform 2).
FT                                /FTId=VSP_001452.
SQ   SEQUENCE   356 AA;  37836 MW;  D346DBAE8392053C CRC64;
     MELTDLLLAA MLLAVARLTL SSPVAPACDP RLLNKLLRDS HLLHSRLSQC PDVDPLSIPV
     LLPAVDFSLG EWKTQTEQSK AQDILGAVSL LLEGVMAARG QLEPSCLSSL LGQLSGQVRL
     LLGALQGLLG TQLPLQGRTT AHKDPNALFL SLQQLLRGKV RFLLLVEGPT LCVRRTLPTT
     AVPSSTSQLL TLNKFPNRTS GLLETNFSVT ARTAGPGLLS RLQGFRVKIT PGQLNQTSRS
     PVQISGYLNR THGPVNGTHG LFAGTSLQTL EASDISPGAF NKGSLAFNLQ GGLPPSPSLA
     PDGHTPFPPS PALPTTHGSP PQLHPLFPDP STTMPNSTAP HPVTMYPHPR NLSQET
//
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