ID TPO_MOUSE Reviewed; 356 AA.
AC P40226;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-APR-2013, entry version 99.
DE RecName: Full=Thrombopoietin;
DE AltName: Full=C-mpl ligand;
DE Short=ML;
DE AltName: Full=Megakaryocyte colony-stimulating factor;
DE AltName: Full=Megakaryocyte growth and development factor;
DE Short=MGDF;
DE AltName: Full=Myeloproliferative leukemia virus oncogene ligand;
DE Flags: Precursor;
GN Name=Thpo;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC Muroidea; Muridae; Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Kidney;
RX PubMed=8202158; DOI=10.1038/369565a0;
RA Lok S., Kaushansky K., Holly R.D., Kuijper J.L., Lofton-Day C.E.,
RA Oort P.J., Grant F.J., Heipel M.D., Burkhead S.K., Kramer J.M.,
RA Bell L.A.N., Sprecher C.A., Blumberg H., Johnson R., Prunkard D.,
RA Ching A.F.T., Mathewes S.L., Bailey M.C., Forstrom J.W., Buddle M.M.,
RA Osborn S.G., Evans S.J., Sheppard P.O., Presnell S.R., O'Hara P.J.,
RA Hagen F.S., Roth G.J., Foster D.C.;
RT "Cloning and expression of murine thrombopoietin cDNA and stimulation
RT of platelet production in vivo.";
RL Nature 369:565-568(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Liver;
RX PubMed=8020099; DOI=10.1016/0092-8674(94)90450-2;
RA Bartley T.D., Bogenberger J., Hunt P., Li Y.-S., Lu H.S., Martin F.,
RA Chang M.-S., Samal B.B., Nichol J.L., Swift S., Johnson M.J.,
RA Hsu R.-Y., Parker V.P., Suggs S., Skrine J.D., Merewether L.A.,
RA Clogson C., Hsu E., Hokom M.M., Hornkohl A., Choi E., Pangelinan M.,
RA Sun Y., Mar V., McNich J., Simonet L., Jacobsen F., Xie C.,
RA Shutter J., Chute H., Basu R., Selander L., Trollinger D., Sieu L.,
RA Padilla D., Trail G., Elliott G., Izumi R., Covey T., Crouse J.,
RA Garcia A., Xu W., del Castillo J., Biron J., Cole S., Hu M.C.-T.,
RA Pacifici R., Ponting I., Saris C., Wen D., Yung Y.P., Lin H.,
RA Bosselman R.A.;
RT "Identification and cloning of a megakaryocyte growth and development
RT factor that is a ligand for the cytokine receptor Mpl.";
RL Cell 77:1117-1124(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RX PubMed=7849319;
RA Gurney A.L., Kuang W.-J., Xie M.-H., Malloy B.E., Eaton D.L.,
RA de Sauvage F.J.;
RT "Genomic structure, chromosomal localization, and conserved
RT alternative splice forms of thrombopoietin.";
RL Blood 85:981-988(1995).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Lineage-specific cytokine affecting the proliferation
CC and maturation of megakaryocytes from their committed progenitor
CC cells. It acts at a late stage of megakaryocyte development. It
CC may be the major physiological regulator of circulating platelets.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=P40226-1; Sequence=Displayed;
CC Name=2; Synonyms=TPO-2;
CC IsoId=P40226-2; Sequence=VSP_001452;
CC -!- TISSUE SPECIFICITY: Found mainly in the liver, kidney and skeletal
CC muscle.
CC -!- DOMAIN: Two-domain structure with an erythropoietin-like N-
CC terminal and a Ser/Pro/Thr-rich C-terminal.
CC -!- SIMILARITY: Belongs to the EPO/TPO family.
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DR EMBL; L34169; AAA40436.1; -; mRNA.
DR EMBL; BC003803; AAH03803.1; -; mRNA.
DR IPI; IPI00108063; -.
DR IPI; IPI00230271; -.
DR PIR; S45330; S45330.
DR RefSeq; NP_001166976.1; NM_001173505.1.
DR RefSeq; NP_033405.1; NM_009379.3.
DR UniGene; Mm.3943; -.
DR ProteinModelPortal; P40226; -.
DR SMR; P40226; 28-172.
DR STRING; 10090.ENSMUSP00000075756; -.
DR PhosphoSite; P40226; -.
DR PRIDE; P40226; -.
DR Ensembl; ENSMUST00000076422; ENSMUSP00000075756; ENSMUSG00000022847.
DR Ensembl; ENSMUST00000115437; ENSMUSP00000111097; ENSMUSG00000022847.
DR GeneID; 21832; -.
DR KEGG; mmu:21832; -.
DR CTD; 7066; -.
DR MGI; MGI:101875; Thpo.
DR eggNOG; NOG84342; -.
DR HOGENOM; HOG000082495; -.
DR HOVERGEN; HBG002468; -.
DR InParanoid; P40226; -.
DR KO; K06854; -.
DR OMA; CPDVNPL; -.
DR OrthoDB; EOG4HT8SV; -.
DR NextBio; 301278; -.
DR ArrayExpress; P40226; -.
DR Bgee; P40226; -.
DR CleanEx; MM_THPO; -.
DR Genevestigator; P40226; -.
DR GermOnline; ENSMUSG00000022847; Mus musculus.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-KW.
DR GO; GO:0008283; P:cell proliferation; IEA:InterPro.
DR GO; GO:0030099; P:myeloid cell differentiation; IDA:MGI.
DR Gene3D; 1.20.1250.10; -; 1.
DR InterPro; IPR009079; 4_helix_cytokine-like_core.
DR InterPro; IPR012351; 4_helix_cytokine_core.
DR InterPro; IPR019767; EPO/TPO_CS.
DR InterPro; IPR001323; EPO_TPO.
DR InterPro; IPR003978; Thrombopoeitin.
DR PANTHER; PTHR10560; PTHR10560; 1.
DR Pfam; PF00758; EPO_TPO; 1.
DR PRINTS; PR01485; THROMBOPTN.
DR SUPFAM; SSF47266; 4_helix_cytokine; 1.
DR PROSITE; PS00817; EPO_TPO; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Complete proteome; Cytokine; Disulfide bond;
KW Glycoprotein; Hormone; Reference proteome; Secreted; Signal.
FT SIGNAL 1 21 Potential.
FT CHAIN 22 356 Thrombopoietin.
FT /FTId=PRO_0000008412.
FT CARBOHYD 197 197 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 206 206 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 235 235 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 249 249 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 256 256 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 336 336 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 351 351 N-linked (GlcNAc...) (Potential).
FT DISULFID 28 172 Potential.
FT DISULFID 50 106 Potential.
FT VAR_SEQ 133 136 Missing (in isoform 2).
FT /FTId=VSP_001452.
SQ SEQUENCE 356 AA; 37836 MW; D346DBAE8392053C CRC64;
MELTDLLLAA MLLAVARLTL SSPVAPACDP RLLNKLLRDS HLLHSRLSQC PDVDPLSIPV
LLPAVDFSLG EWKTQTEQSK AQDILGAVSL LLEGVMAARG QLEPSCLSSL LGQLSGQVRL
LLGALQGLLG TQLPLQGRTT AHKDPNALFL SLQQLLRGKV RFLLLVEGPT LCVRRTLPTT
AVPSSTSQLL TLNKFPNRTS GLLETNFSVT ARTAGPGLLS RLQGFRVKIT PGQLNQTSRS
PVQISGYLNR THGPVNGTHG LFAGTSLQTL EASDISPGAF NKGSLAFNLQ GGLPPSPSLA
PDGHTPFPPS PALPTTHGSP PQLHPLFPDP STTMPNSTAP HPVTMYPHPR NLSQET
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