ID TS1R3_RAT Reviewed; 858 AA.
AC Q923K1;
DT 04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 24-JAN-2024, entry version 126.
DE RecName: Full=Taste receptor type 1 member 3;
DE AltName: Full=Sweet taste receptor T1R3;
DE Flags: Precursor;
GN Name=Tas1r3; Synonyms=T1r3, Tr3;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Wistar; TISSUE=Circumvallate papilla;
RX PubMed=11509186; DOI=10.1016/s0092-8674(01)00451-2;
RA Nelson G., Hoon M.A., Chandrashekar J., Zhang Y., Ryba N.J.P., Zuker C.S.;
RT "Mammalian sweet taste receptors.";
RL Cell 106:381-390(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Wistar; TISSUE=Circumvallate papilla;
RX PubMed=11917125; DOI=10.1073/pnas.072090199;
RA Li X., Staszewski L., Xu H., Durick K., Zoller M., Adler E.;
RT "Human receptors for sweet and umami taste.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:4692-4696(2002).
CC -!- FUNCTION: Putative taste receptor. TAS1R1/TAS1R3 responds to the umami
CC taste stimulus (the taste of monosodium glutamate) and also to most of
CC the 20 standard L-amino acids, but not to their D-enantiomers or other
CC compounds. TAS1R2/TAS1R3 recognizes diverse natural and synthetic
CC sweeteners. TAS1R3 is essential for the recognition and response to the
CC disaccharide trehalose. Sequence differences within and between species
CC can significantly influence the selectivity and specificity of taste
CC responses (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Forms homodimers or heterodimers with TAS1R1 and TAS1R2.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 3 family. TAS1R
CC subfamily. {ECO:0000305}.
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DR EMBL; AY032620; AAK51601.1; -; mRNA.
DR EMBL; AF456324; AAM10636.1; -; mRNA.
DR RefSeq; NP_570831.1; NM_130818.1.
DR RefSeq; XP_008762556.1; XM_008764334.1.
DR AlphaFoldDB; Q923K1; -.
DR SMR; Q923K1; -.
DR STRING; 10116.ENSRNOP00000026671; -.
DR GlyCosmos; Q923K1; 9 sites, No reported glycans.
DR GlyGen; Q923K1; 9 sites.
DR PaxDb; 10116-ENSRNOP00000026671; -.
DR Ensembl; ENSRNOT00000026671.3; ENSRNOP00000026671.1; ENSRNOG00000019589.3.
DR Ensembl; ENSRNOT00055023637; ENSRNOP00055019241; ENSRNOG00055013753.
DR Ensembl; ENSRNOT00060007624; ENSRNOP00060005712; ENSRNOG00060004571.
DR Ensembl; ENSRNOT00065016486; ENSRNOP00065012530; ENSRNOG00065010226.
DR GeneID; 170634; -.
DR KEGG; rno:170634; -.
DR AGR; RGD:620539; -.
DR CTD; 83756; -.
DR RGD; 620539; Tas1r3.
DR eggNOG; KOG1056; Eukaryota.
DR GeneTree; ENSGT00940000160679; -.
DR HOGENOM; CLU_005389_1_0_1; -.
DR InParanoid; Q923K1; -.
DR OMA; WALGMKF; -.
DR OrthoDB; 4096532at2759; -.
DR PhylomeDB; Q923K1; -.
DR TreeFam; TF331269; -.
DR Reactome; R-RNO-418594; G alpha (i) signalling events.
DR Reactome; R-RNO-420499; Class C/3 (Metabotropic glutamate/pheromone receptors).
DR Reactome; R-RNO-9717207; Sensory perception of sweet, bitter, and umami (glutamate) taste.
DR PRO; PR:Q923K1; -.
DR Proteomes; UP000002494; Chromosome 5.
DR Bgee; ENSRNOG00000019589; Expressed in skeletal muscle tissue and 2 other cell types or tissues.
DR Genevisible; Q923K1; RN.
DR GO; GO:0005794; C:Golgi apparatus; IEA:Ensembl.
DR GO; GO:0016020; C:membrane; IC:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:1903767; C:sweet taste receptor complex; ISO:RGD.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IBA:GO_Central.
DR GO; GO:0033041; F:sweet taste receptor activity; IEA:Ensembl.
DR GO; GO:0008527; F:taste receptor activity; IDA:RGD.
DR GO; GO:0001582; P:detection of chemical stimulus involved in sensory perception of sweet taste; ISO:RGD.
DR GO; GO:0050916; P:sensory perception of sweet taste; IDA:UniProtKB.
DR GO; GO:0050917; P:sensory perception of umami taste; IDA:UniProtKB.
DR Gene3D; 3.40.50.2300; -; 2.
DR Gene3D; 2.10.50.30; GPCR, family 3, nine cysteines domain; 1.
DR InterPro; IPR001828; ANF_lig-bd_rcpt.
DR InterPro; IPR000337; GPCR_3.
DR InterPro; IPR011500; GPCR_3_9-Cys_dom.
DR InterPro; IPR038550; GPCR_3_9-Cys_sf.
DR InterPro; IPR017978; GPCR_3_C.
DR InterPro; IPR000068; GPCR_3_Ca_sens_rcpt-rel.
DR InterPro; IPR017979; GPCR_3_CS.
DR InterPro; IPR028082; Peripla_BP_I.
DR PANTHER; PTHR24061; CALCIUM-SENSING RECEPTOR-RELATED; 1.
DR PANTHER; PTHR24061:SF435; TASTE RECEPTOR TYPE 1 MEMBER 3; 1.
DR Pfam; PF00003; 7tm_3; 1.
DR Pfam; PF01094; ANF_receptor; 1.
DR Pfam; PF07562; NCD3G; 1.
DR PRINTS; PR00592; CASENSINGR.
DR PRINTS; PR00248; GPCRMGR.
DR SUPFAM; SSF53822; Periplasmic binding protein-like I; 1.
DR PROSITE; PS00980; G_PROTEIN_RECEP_F3_2; 1.
DR PROSITE; PS50259; G_PROTEIN_RECEP_F3_4; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; G-protein coupled receptor; Glycoprotein; Membrane;
KW Receptor; Reference proteome; Sensory transduction; Signal; Taste;
KW Transducer; Transmembrane; Transmembrane helix.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..858
FT /note="Taste receptor type 1 member 3"
FT /id="PRO_0000012964"
FT TOPO_DOM 21..575
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 576..596
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 597..610
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 611..631
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 632..644
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 645..665
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 666..687
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 688..708
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 709..735
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 736..756
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 757..767
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 768..788
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 789..796
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 797..817
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 818..858
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 839..858
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 842..858
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 85
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 130
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 203
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 264
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 379
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 387
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 418
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 439
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 482
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 858 AA; 94846 MW; 98890DAF75973B80 CRC64;
MPGLAILGLS LAAFLELGMG SSLCLSQQFK AQGDYILGGL FPLGTTEEAT LNQRTQPNGI
LCTRFSPLGL FLAMAMKMAV EEINNGSALL PGLRLGYDLF DTCSEPVVTM KPSLMFMAKV
GSQSIAAYCN YTQYQPRVLA VIGPHSSELA LITGKFFSFF LMPQVSYSAS MDRLSDRETF
PSFFRTVPSD RVQLQAVVTL LQNFSWNWVA ALGSDDDYGR EGLSIFSGLA NSRGICIAHE
GLVPQHDTSG QQLGKVVDVL RQVNQSKVQV VVLFASARAV YSLFSYSILH DLSPKVWVAS
ESWLTSDLVM TLPNIARVGT VLGFLQRGAL LPEFSHYVET RLALAADPTF CASLKAELDL
EERVMGPRCS QCDYIMLQNL SSGLMQNLSA GQLHHQIFAT YAAVYSVAQA LHNTLQCNVS
HCHTSEPVQP WQLLENMYNM SFRARDLTLQ FDAKGSVDME YDLKMWVWQS PTPVLHTVGT
FNGTLQLQHS KMYWPGNQVP VSQCSRQCKD GQVRRVKGFH SCCYDCVDCK AGSYRKHPDD
FTCTPCGKDQ WSPEKSTTCL PRRPKFLAWG EPAVLSLLLL LCLVLGLTLA ALGLFVHYWD
SPLVQASGGS LFCFGLICLG LFCLSVLLFP GRPRSASCLA QQPMAHLPLT GCLSTLFLQA
AEIFVESELP LSWANWLCSY LRGPWAWLVV LLATLVEAAL CAWYLMAFPP EVVTDWQVLP
TEVLEHCRMR SWVSLGLVHI TNAVLAFLCF LGTFLVQSQP GRYNRARGLT FAMLAYFIIW
VSFVPLLANV QVAYQPAVQM GAILFCALGI LATFHLPKCY VLLWLPELNT QEFFLGRSPK
EASDGNSGSS EATRGHSE
//
