ID TUSA_BUCAP Reviewed; 76 AA.
AC Q8K9B6;
DT 08-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2002, sequence version 2.
DT 27-MAR-2024, entry version 104.
DE RecName: Full=Sulfur carrier protein TusA {ECO:0000255|HAMAP-Rule:MF_00413};
DE AltName: Full=Sulfur mediator TusA {ECO:0000255|HAMAP-Rule:MF_00413};
DE AltName: Full=Sulfur transfer protein TusA {ECO:0000255|HAMAP-Rule:MF_00413};
DE AltName: Full=tRNA 2-thiouridine synthesizing protein A {ECO:0000255|HAMAP-Rule:MF_00413};
GN Name=tusA {ECO:0000255|HAMAP-Rule:MF_00413}; OrderedLocusNames=BUsg_432;
OS Buchnera aphidicola subsp. Schizaphis graminum (strain Sg).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Erwiniaceae; Buchnera.
OX NCBI_TaxID=198804;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Sg;
RX PubMed=12089438; DOI=10.1126/science.1071278;
RA Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S.,
RA Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.;
RT "50 million years of genomic stasis in endosymbiotic bacteria.";
RL Science 296:2376-2379(2002).
CC -!- FUNCTION: Sulfur carrier protein involved in sulfur trafficking in the
CC cell. Part of a sulfur-relay system required for 2-thiolation during
CC synthesis of 2-thiouridine of the modified wobble base 5-
CC methylaminomethyl-2-thiouridine (mnm(5)s(2)U) in tRNA. Interacts with
CC IscS and stimulates its cysteine desulfurase activity. Accepts an
CC activated sulfur from IscS, which is then transferred to TusD, and thus
CC determines the direction of sulfur flow from IscS to 2-thiouridine
CC formation. Also appears to be involved in sulfur transfer for the
CC biosynthesis of molybdopterin. {ECO:0000255|HAMAP-Rule:MF_00413}.
CC -!- PATHWAY: tRNA modification. {ECO:0000255|HAMAP-Rule:MF_00413}.
CC -!- SUBUNIT: Interacts with IscS. {ECO:0000255|HAMAP-Rule:MF_00413}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00413}.
CC -!- SIMILARITY: Belongs to the sulfur carrier protein TusA family.
CC {ECO:0000255|HAMAP-Rule:MF_00413}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM67975.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AE013218; AAM67975.1; ALT_FRAME; Genomic_DNA.
DR AlphaFoldDB; Q8K9B6; -.
DR SMR; Q8K9B6; -.
DR STRING; 198804.BUsg_432; -.
DR KEGG; bas:BUsg_432; -.
DR eggNOG; COG0425; Bacteria.
DR HOGENOM; CLU_165255_5_1_6; -.
DR Proteomes; UP000000416; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0097163; F:sulfur carrier activity; IEA:UniProtKB-UniRule.
DR GO; GO:0002143; P:tRNA wobble position uridine thiolation; IEA:InterPro.
DR CDD; cd03423; SirA; 1.
DR Gene3D; 3.30.110.40; TusA-like domain; 1.
DR HAMAP; MF_00413; Thiourid_synth_A; 1.
DR InterPro; IPR022931; Sulphur_carrier_TusA.
DR InterPro; IPR001455; TusA-like.
DR InterPro; IPR036868; TusA-like_sf.
DR PANTHER; PTHR33279:SF6; SULFUR CARRIER PROTEIN TUSA; 1.
DR PANTHER; PTHR33279; SULFUR CARRIER PROTEIN YEDF-RELATED; 1.
DR Pfam; PF01206; TusA; 1.
DR SUPFAM; SSF64307; SirA-like; 1.
DR PROSITE; PS01148; UPF0033; 1.
PE 3: Inferred from homology;
KW Cytoplasm; tRNA processing.
FT CHAIN 1..76
FT /note="Sulfur carrier protein TusA"
FT /id="PRO_0000159031"
FT ACT_SITE 15
FT /note="Cysteine persulfide intermediate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00413"
SQ SEQUENCE 76 AA; 9206 MW; EA4CA273CEC03D7B CRC64;
MEKKNIILNL IGLRCPEPIM VIRKTLRKMK KNEKILVLAD DPSTKRDIPY FCYFMEHQLL
DRFIHVKPYR YLLKKG
//
