ID U1MZ53_9BACL Unreviewed; 1142 AA.
AC U1MZ53;
DT 13-NOV-2013, integrated into UniProtKB/TrEMBL.
DT 13-NOV-2013, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE RecName: Full=Alpha-amylase {ECO:0008006|Google:ProtNLM};
GN ORFNames=M467_07410 {ECO:0000313|EMBL:ERG67106.1};
OS Exiguobacterium chiriqhucha RW-2.
OC Bacteria; Bacillota; Bacilli; Bacillales;
OC Bacillales Family XII. Incertae Sedis; Exiguobacterium.
OX NCBI_TaxID=1345023 {ECO:0000313|EMBL:ERG67106.1, ECO:0000313|Proteomes:UP000016464};
RN [1] {ECO:0000313|EMBL:ERG67106.1, ECO:0000313|Proteomes:UP000016464}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RW-2 {ECO:0000313|EMBL:ERG67106.1,
RC ECO:0000313|Proteomes:UP000016464};
RX PubMed=23929485;
RA White R.A.III., Grassa C.J., Suttle C.A.;
RT "Draft Genome Sequence of Exiguobacterium pavilionensis Strain RW-2, with
RT Wide Thermal, Salinity, and pH Tolerance, Isolated from Modern Freshwater
RT Microbialites.";
RL Genome Announc. 1:e00597-e00513(2013).
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ERG67106.1}.
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DR EMBL; ATCL01000019; ERG67106.1; -; Genomic_DNA.
DR RefSeq; WP_021066749.1; NZ_ATCL01000019.1.
DR AlphaFoldDB; U1MZ53; -.
DR STRING; 1385984.GCA_000702565_03028; -.
DR PATRIC; fig|1345023.5.peg.1590; -.
DR eggNOG; COG0366; Bacteria.
DR eggNOG; COG5492; Bacteria.
DR OrthoDB; 9761875at2; -.
DR Proteomes; UP000016464; Unassembled WGS sequence.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd10315; CBM41_pullulanase; 1.
DR Gene3D; 2.60.40.1110; -; 1.
DR Gene3D; 2.60.40.1220; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR031319; A-amylase_C.
DR InterPro; IPR013784; Carb-bd-like_fold.
DR InterPro; IPR005323; CBM41_pullulanase.
DR InterPro; IPR014755; Cu-Rt/internalin_Ig-like.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR032812; SbsA_Ig.
DR PANTHER; PTHR10357:SF215; ALPHA-AMYLASE 1; 1.
DR PANTHER; PTHR10357; ALPHA-AMYLASE FAMILY MEMBER; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF13205; Big_5; 1.
DR Pfam; PF03714; PUD; 1.
DR SMART; SM00642; Aamy; 1.
DR SMART; SM00632; Aamy_C; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
DR SUPFAM; SSF49452; Starch-binding domain-like; 1.
PE 4: Predicted;
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000016464};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..30
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 31..1142
FT /note="Alpha-amylase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5004618037"
FT DOMAIN 661..1044
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT DOMAIN 1056..1141
FT /note="Alpha-amylase C-terminal"
FT /evidence="ECO:0000259|SMART:SM00632"
SQ SEQUENCE 1142 AA; 126383 MW; ADC262CC02AC194F CRC64;
MKKKTARQTT TLLLSSVLLI QAGVPMNALA EPATISIDGL KTDWANTPAL ATSPQAGWQG
FDLGDVYLQN DARNLYFYVD ANNVPNWGDN GQYINIALQI NEQDSGVATN PLGHPFNFSQ
TDKKPQYHIL LRVDGDTSVK EAALYEAGKS TPLANLSNLN GASFAIDRTK GFEGKIPLSL
LGLSNDDTVR VLTVLSGNNA TEHGAFDTIP SNAANKVADS WNVSATPSTQ SVYSPAFTLS
GVETVSQLEV TAVTPANQSE QVDIKSPITW TFNEPVSLDA SNISLMNGET AVPFTAEASG
STVTVTPTEA LSLNQTYTAT LPADAVKGNL SNTTLPALTT SFKTITEIAD PWKTQRYIEM
LYVRADNDYT DWNLWTWSTG AKDGQVDPYK ITEDGAIFRI PVGKDATNVG FVIRKGTDWA
VKDGYGEDRY VKLGSDRVTK VVVESGKGTF HQVPTIQGPL FEDGAVTFFY RDKALYEAGQ
LDEIDSVALK VNGTTYPMTY EAKNEWFRHT VKLPEGKHDY TFLVTKDGVT TEVKDPYFEA
SSVEYRRPNV KLAASVAPKA LSSRENAVVQ VKPTLPKGVK LRQLFIDARP LGGPAKLEVD
PALNKQTIAV KDTVKPGNKF LTITAIDQYG NVHKTKTSLT VRPVSMKDKQ EFDWDEARIY
FMLTDRFYDG DKTNNNPNGE YYDQDHLESY HGGDFAGVTK KLDYLDKLGI NTIWITPIVD
NIDWDLRYGK DGSQYGYHGY WAKNFEKLDE HLGDMKAFHR LIDEANARGI KIMVDVVLNH
PGYGMEPGAV DTGATNFPTD QERKAFDGMI RSNPVDGDDL KMSLSGLPDF KTEEAAVREQ
LVKWQTDWIK RSKTKKGNTI DYFRVDTVKH VDSTTWKSFK NELTALKPDF KMIGEHYGAS
VNNTGGYLQS GQMDSLLDFD FKYQAERFIN GNIDDVESAL QYRNTQLSNE ATLGQFLSSH
DEDGFLVSRA GGDEGKHMVA ASLQMTAKGQ PVIYYGEEVG QSGKHALDMD KGEFNENRYD
FDWKRVKGEG KAMHTHYQKL LNIRADYSKV FSKGTRAVVA GGSATGYSVF ERKHGKDSVL
VALNTTEAKR TITFQTKLKH KTTLVDRYSG RHYVVKRDGK VTVSLPAQDD GGTVILVAKS
KK
//
