ID U1NVR3_9EURY Unreviewed; 328 AA.
AC U1NVR3;
DT 13-NOV-2013, integrated into UniProtKB/TrEMBL.
DT 13-NOV-2013, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE RecName: Full=5,10-methylenetetrahydromethanopterin reductase {ECO:0000256|HAMAP-Rule:MF_01091};
DE EC=1.5.98.2 {ECO:0000256|HAMAP-Rule:MF_01091};
DE AltName: Full=Coenzyme F420-dependent N(5),N(10)-methylenetetrahydromethanopterin reductase {ECO:0000256|HAMAP-Rule:MF_01091};
DE AltName: Full=Methylene-H(4)MPT reductase {ECO:0000256|HAMAP-Rule:MF_01091};
GN Name=mer {ECO:0000256|HAMAP-Rule:MF_01091};
GN ORFNames=J07HN4v3_00516 {ECO:0000313|EMBL:ERH08150.1};
OS Halonotius sp. J07HN4.
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales;
OC Halorubraceae; Halonotius.
OX NCBI_TaxID=1070774 {ECO:0000313|EMBL:ERH08150.1, ECO:0000313|Proteomes:UP000030639};
RN [1] {ECO:0000313|EMBL:ERH08150.1, ECO:0000313|Proteomes:UP000030639}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=J07HN4 {ECO:0000313|Proteomes:UP000030639};
RX PubMed=23637883;
RA Podell S., Ugalde J.A., Narasingarao P., Banfield J.F., Heidelberg K.B.,
RA Allen E.E.;
RT "Assembly-driven community genomics of a hypersaline microbial ecosystem.";
RL PLoS ONE 8:E61692-E61692(2013).
CC -!- FUNCTION: Catalyzes the oxidation of methyl-H(4)MPT to methylene-
CC H(4)MPT. {ECO:0000256|HAMAP-Rule:MF_01091}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5-methyl-5,6,7,8-tetrahydromethanopterin + H(+) + oxidized
CC coenzyme F420-(gamma-L-Glu)(n) = 5,10-
CC methylenetetrahydromethanopterin + reduced coenzyme F420-(gamma-L-
CC Glu)(n); Xref=Rhea:RHEA:21144, Rhea:RHEA-COMP:12939, Rhea:RHEA-
CC COMP:14378, ChEBI:CHEBI:15378, ChEBI:CHEBI:57818, ChEBI:CHEBI:58116,
CC ChEBI:CHEBI:133980, ChEBI:CHEBI:139511; EC=1.5.98.2;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01091};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_01091}.
CC -!- SIMILARITY: Belongs to the mer family. {ECO:0000256|HAMAP-
CC Rule:MF_01091}.
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DR EMBL; KE356577; ERH08150.1; -; Genomic_DNA.
DR AlphaFoldDB; U1NVR3; -.
DR eggNOG; arCOG02410; Archaea.
DR HOGENOM; CLU_027853_5_3_2; -.
DR Proteomes; UP000030639; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0018537; F:coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR CDD; cd01097; Tetrahydromethanopterin_reductase; 1.
DR Gene3D; 3.20.20.30; Luciferase-like domain; 1.
DR HAMAP; MF_01091; F420_mer; 1.
DR InterPro; IPR011251; Luciferase-like_dom.
DR InterPro; IPR036661; Luciferase-like_sf.
DR InterPro; IPR019946; MeH4methanopterin_reductase.
DR PANTHER; PTHR43244; -; 1.
DR PANTHER; PTHR43244:SF1; 5,10-METHYLENETETRAHYDROMETHANOPTERIN REDUCTASE; 1.
DR Pfam; PF00296; Bac_luciferase; 1.
DR SUPFAM; SSF51679; Bacterial luciferase-like; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_01091};
KW One-carbon metabolism {ECO:0000256|ARBA:ARBA00022563, ECO:0000256|HAMAP-
KW Rule:MF_01091}; Oxidoreductase {ECO:0000256|HAMAP-Rule:MF_01091};
KW Reference proteome {ECO:0000313|Proteomes:UP000030639}.
FT DOMAIN 9..295
FT /note="Luciferase-like"
FT /evidence="ECO:0000259|Pfam:PF00296"
SQ SEQUENCE 328 AA; 34196 MW; 3100CD80E884EB69 CRC64;
MFGVEITPEH PIDRITDLGV AAEAAGYDTL FVSSHYNNRD PFAALQRIAV ETDEIALGPG
VANPFETHPV TLASKVATVD ETADGRGVFG IGPGDPSTVA NLGFADDRGL RPVLEAFKTA
QKLWAGERVD NDGTFDADDA GLNYEPPSGA DIPVYVGGEG PHMCRMAAKH ADGLLFNGSH
AADLAWAREE SNKGEADRPD HRGEFDLAAY ASVSVADERA AAREAARPPV AYITAGAAPP
VLDRHDIDGE IADEIGEKIS AGEFSAAFDL VTPAMIDAFC LVGAVDDVAD QMAAVLEHAD
SIVAGSPLGP DLEAAVELAA EAHAQATN
//
