ID U1QC47_9ACTO Unreviewed; 520 AA.
AC U1QC47;
DT 13-NOV-2013, integrated into UniProtKB/TrEMBL.
DT 13-NOV-2013, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE SubName: Full=Putative cytoplasmic alpha-amylase {ECO:0000313|EMBL:ERH19549.1};
GN ORFNames=HMPREF0043_00574 {ECO:0000313|EMBL:ERH19549.1};
OS Actinobaculum sp. oral taxon 183 str. F0552.
OC Bacteria; Actinomycetota; Actinomycetes; Actinomycetales; Actinomycetaceae;
OC Actinobaculum.
OX NCBI_TaxID=1227261 {ECO:0000313|EMBL:ERH19549.1, ECO:0000313|Proteomes:UP000016495};
RN [1] {ECO:0000313|EMBL:ERH19549.1, ECO:0000313|Proteomes:UP000016495}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=F0552 {ECO:0000313|EMBL:ERH19549.1,
RC ECO:0000313|Proteomes:UP000016495};
RA Weinstock G., Sodergren E., Lobos E.A., Fulton L., Fulton R., Courtney L.,
RA Fronick C., O'Laughlin M., Godfrey J., Wilson R.M., Miner T., Farmer C.,
RA Delehaunty K., Cordes M., Minx P., Tomlinson C., Chen J., Wollam A.,
RA Pepin K.H., Bhonagiri V., Zhang X., Warren W., Mitreva M., Mardis E.R.,
RA Wilson R.K.;
RL Submitted (JUN-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ERH19549.1}.
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DR EMBL; AWSB01000016; ERH19549.1; -; Genomic_DNA.
DR AlphaFoldDB; U1QC47; -.
DR STRING; 1227261.HMPREF0043_00574; -.
DR PATRIC; fig|1227261.3.peg.503; -.
DR eggNOG; COG0366; Bacteria.
DR HOGENOM; CLU_024572_2_1_11; -.
DR Proteomes; UP000016495; Unassembled WGS sequence.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11318; AmyAc_bac_fung_AmyA; 1.
DR Gene3D; 2.40.30.140; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013776; A-amylase_thermo.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR43447; ALPHA-AMYLASE; 1.
DR PANTHER; PTHR43447:SF49; ALPHA-AMYLASE; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR PIRSF; PIRSF001021; Alph-amls_thrmst; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 4: Predicted;
KW Calcium {ECO:0000256|PIRSR:PIRSR001021-2};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR001021-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000016495}.
FT DOMAIN 26..414
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..19
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 253
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT ACT_SITE 283
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT BINDING 126
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT BINDING 216
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT BINDING 222
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT BINDING 257
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT BINDING 321
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT BINDING 425
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT BINDING 448
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
SQ SEQUENCE 520 AA; 56704 MW; E07BA836ACABA6E4 CRC64;
MNFERTDGMD NRDRTGAERA PEAANPLMLQ AFAWDLNADS SHWRLLAENA AFLAACGVSS
MWLPPAYKGQ AGVKDVGYGV YDTYDLGEFD QKGTVPTKYG TKDEYLAAIE ALHAAGIAVV
ADIVLNHRMG GEATETVRAT PVDPADRRRP IGEPEDITAW TRFTFPGRAG AYSDFAWDWT
CFHGIDWDEA RQLAGVWLFE GKQWNENVDD ELGNYDYLMG ADVHATDPKV RAELDRWGRW
YVETTGVDGL RLDAVKHVGA DFFASWLPEL RRATGRALPA VGEYWSQNVA ELKEYLQAVP
STSLFDVPLH FHLHAASTSN GDVDLSRLFE GTLVEADPAR AVTFVENHDT QPGQSLASTI
QPWFKPSAYA LILLRDAGTP CVFWGDLFGT PETGDLPAVT ELPMLMTMRR ALAHGPQHDA
LDAPDVVGFA REGDAAHPGS GLAVVLSDRL AGTKRLHVGA RHAGERWVCV LGGHEPVTVG
DDGTAELPVS DGGLSVYVPE AAKPILDGSE EYRLRVGSGD
//
