ID U2KCZ4_9FIRM Unreviewed; 388 AA.
AC U2KCZ4;
DT 13-NOV-2013, integrated into UniProtKB/TrEMBL.
DT 13-NOV-2013, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE SubName: Full=Thiazole biosynthesis protein ThiH {ECO:0000313|EMBL:ERJ90102.1};
GN ORFNames=RUMCAL_02872 {ECO:0000313|EMBL:ERJ90102.1};
OS Ruminococcus callidus ATCC 27760.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae;
OC Ruminococcus.
OX NCBI_TaxID=411473 {ECO:0000313|EMBL:ERJ90102.1, ECO:0000313|Proteomes:UP000016662};
RN [1] {ECO:0000313|EMBL:ERJ90102.1, ECO:0000313|Proteomes:UP000016662}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27760 {ECO:0000313|EMBL:ERJ90102.1,
RC ECO:0000313|Proteomes:UP000016662};
RA Weinstock G., Sodergren E., Wylie T., Fulton L., Fulton R., Fronick C.,
RA O'Laughlin M., Godfrey J., Miner T., Herter B., Appelbaum E., Cordes M.,
RA Lek S., Wollam A., Pepin K.H., Palsikar V.B., Mitreva M., Wilson R.K.;
RL Submitted (JUL-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ERJ90102.1}.
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DR EMBL; AWVF01000367; ERJ90102.1; -; Genomic_DNA.
DR RefSeq; WP_021681059.1; NZ_KI260329.1.
DR AlphaFoldDB; U2KCZ4; -.
DR STRING; 411473.RUMCAL_02872; -.
DR GeneID; 78474595; -.
DR PATRIC; fig|411473.3.peg.2407; -.
DR eggNOG; COG0502; Bacteria.
DR HOGENOM; CLU_046249_1_0_9; -.
DR OrthoDB; 9801120at2; -.
DR Proteomes; UP000016662; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0009228; P:thiamine biosynthetic process; IEA:InterPro.
DR CDD; cd01335; Radical_SAM; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR010722; BATS_dom.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR012726; ThiH.
DR InterPro; IPR034428; ThiH/NoCL/HydG-like.
DR NCBIfam; TIGR02351; thiH; 1.
DR PANTHER; PTHR43583; 2-IMINOACETATE SYNTHASE; 1.
DR PANTHER; PTHR43583:SF1; 2-IMINOACETATE SYNTHASE; 1.
DR Pfam; PF06968; BATS; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDF00301; 2-iminoacetate_synthase_(ThiH); 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SMART; SM00876; BATS; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}.
FT DOMAIN 85..302
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 388 AA; 43956 MW; 410154E5073CBF2B CRC64;
MRKIYDPMEY QPNMQQIDSD IFDHVIAART AYQPEQYTAK QVQAALRKDV LSFEDFAALL
SPAAQPFLEQ MARRAKEERE KHFGNNVLLF TPLYISNYCE NQCIYCGFNC KNPITRCKLN
AAEIEAEMEN IAKTGMREVL LLTGESRSVS GPEYIAEAVR IAKKYFSTIG LEVYPMNADE
YAMLREAGAD FVTVFQETYD LQRYGEMHVA GAKRCFPYRV NAQERALMGG MRGVGFGVLL
GLADFRKDMF AAGVHAALLQ KKYPQGEMAL SFPRLRPYKN HEETNPNDVH ETQLLQMMLA
CRIFLPFASF TISTRERADF RDHVLPLAAT KISAGVNVGI GGHNEEHQQK GDAQFEISDP
RSLEEIHNAI LAQGMQPVYT DYINTNIL
//
