ID U3KRG6_9PICO Unreviewed; 2177 AA.
AC U3KRG6;
DT 13-NOV-2013, integrated into UniProtKB/TrEMBL.
DT 13-NOV-2013, sequence version 1.
DT 27-MAR-2024, entry version 59.
DE RecName: Full=Genome polyprotein {ECO:0000256|ARBA:ARBA00020107};
DE Flags: Fragment;
OS Human parechovirus 3.
OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC Picornavirales; Picornaviridae; Paavivirinae; Parechovirus; Parechovirus A.
OX NCBI_TaxID=195055 {ECO:0000313|EMBL:BAM64935.1};
RN [1] {ECO:0000313|EMBL:BAM64935.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=1588-Yamagata-2011 {ECO:0000313|EMBL:BAM64935.1};
RA Mizuta K., Yamakawa T., Nagasawa H., Itagaki T., Katsushima F.,
RA Katsushima Y., Shimizu Y., Ito S., Aoki Y., Ikeda T., Abiko C., Kuroda M.,
RA Noda M., Kimura H., Ahiko T.;
RT "Epidemic myalgia associated with human parechovirus type 3 infection among
RT adults occurs during an outbreak among children: Findings from Yamagata,
RT Japan, in 2011.";
RL J. Clin. Virol. 58:188-193(2013).
RN [2] {ECO:0007829|PDB:8A2E}
RP X-RAY CRYSTALLOGRAPHY (2.29 ANGSTROMS) OF 772-918.
RA von Castelmur E., Perrakis A.;
RT "Structural plasticity of 2A proteins in the Parechovirus family.";
RL Submitted (JUN-2022) to the PDB data bank.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane
CC {ECO:0000256|ARBA:ARBA00004180}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004180}; Cytoplasmic side
CC {ECO:0000256|ARBA:ARBA00004180}. Host cytoplasmic vesicle membrane
CC {ECO:0000256|ARBA:ARBA00004295}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004295}; Cytoplasmic side
CC {ECO:0000256|ARBA:ARBA00004295}. Membrane
CC {ECO:0000256|ARBA:ARBA00004287}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004287}; Cytoplasmic side
CC {ECO:0000256|ARBA:ARBA00004287}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB759194; BAM64935.1; -; Genomic_RNA.
DR PDB; 8A2E; X-ray; 2.29 A; AAA/BBB/CCC/DDD/EEE/FFF/GGG/HHH=772-918.
DR SMR; U3KRG6; -.
DR GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0044162; C:host cell cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0039618; C:T=pseudo3 icosahedral viral capsid; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0005216; F:monoatomic ion channel activity; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003724; F:RNA helicase activity; IEA:InterPro.
DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR GO; GO:0051259; P:protein complex oligomerization; IEA:UniProtKB-KW.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR GO; GO:0019062; P:virion attachment to host cell; IEA:UniProtKB-KW.
DR GO; GO:0039707; P:virus-mediated pore formation in host cell membrane; IEA:UniProtKB-KW.
DR CDD; cd23217; Parechovirus_RdRp; 1.
DR CDD; cd00205; rhv_like; 2.
DR Gene3D; 2.60.120.20; -; 3.
DR Gene3D; 3.30.70.270; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 2.40.10.10; Trypsin-like serine proteases; 1.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR004004; Helic/Pol/Pept_Calicivir-typ.
DR InterPro; IPR000605; Helicase_SF3_ssDNA/RNA_vir.
DR InterPro; IPR014759; Helicase_SF3_ssRNA_vir.
DR InterPro; IPR007053; LRAT_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR044067; PCV_3C_PRO.
DR InterPro; IPR000199; Peptidase_C3A/C3B_picornavir.
DR InterPro; IPR009003; Peptidase_S1_PA.
DR InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR InterPro; IPR001676; Picornavirus_capsid.
DR InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR InterPro; IPR033703; Rhv-like.
DR InterPro; IPR001205; RNA-dir_pol_C.
DR InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR InterPro; IPR029053; Viral_coat.
DR InterPro; IPR009419; VPP_parechovir_P3A.
DR InterPro; IPR009407; VPP_parechovir_P3B.
DR Pfam; PF06344; Parecho_VpG; 1.
DR Pfam; PF00548; Peptidase_C3; 1.
DR Pfam; PF06363; Picorna_P3A; 1.
DR Pfam; PF00680; RdRP_1; 1.
DR Pfam; PF00073; Rhv; 1.
DR Pfam; PF00910; RNA_helicase; 1.
DR PRINTS; PR00918; CALICVIRUSNS.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF88633; Positive stranded ssRNA viruses; 3.
DR SUPFAM; SSF50494; Trypsin-like serine proteases; 1.
DR PROSITE; PS51934; LRAT; 1.
DR PROSITE; PS51874; PCV_3C_PRO; 1.
DR PROSITE; PS50507; RDRP_SSRNA_POS; 1.
DR PROSITE; PS51218; SF3_HELICASE_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure {ECO:0007829|PDB:8A2E};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Capsid protein {ECO:0000256|ARBA:ARBA00022561};
KW Covalent protein-RNA linkage {ECO:0000256|ARBA:ARBA00022520};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Host cytoplasm {ECO:0000256|ARBA:ARBA00023200};
KW Host cytoplasmic vesicle {ECO:0000256|ARBA:ARBA00022488};
KW Host membrane {ECO:0000256|ARBA:ARBA00022870};
KW Host-virus interaction {ECO:0000256|ARBA:ARBA00022581};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Ion channel {ECO:0000256|ARBA:ARBA00023303};
KW Ion transport {ECO:0000256|ARBA:ARBA00023065};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484};
KW T=pseudo3 icosahedral capsid protein {ECO:0000256|ARBA:ARBA00022706};
KW Thiol protease {ECO:0000256|ARBA:ARBA00022807};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transport {ECO:0000256|ARBA:ARBA00022448};
KW Viral attachment to host cell {ECO:0000256|ARBA:ARBA00022804};
KW Viral ion channel {ECO:0000256|ARBA:ARBA00023039};
KW Viral RNA replication {ECO:0000256|ARBA:ARBA00022953};
KW Virion {ECO:0000256|ARBA:ARBA00022706};
KW Virus entry into host cell {ECO:0000256|ARBA:ARBA00023296}.
FT DOMAIN 784..878
FT /note="LRAT"
FT /evidence="ECO:0000259|PROSITE:PS51934"
FT DOMAIN 1154..1315
FT /note="SF3 helicase"
FT /evidence="ECO:0000259|PROSITE:PS51218"
FT DOMAIN 1515..1705
FT /note="Peptidase C3"
FT /evidence="ECO:0000259|PROSITE:PS51874"
FT DOMAIN 1942..2056
FT /note="RdRp catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50507"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:BAM64935.1"
FT NON_TER 2177
FT /evidence="ECO:0000313|EMBL:BAM64935.1"
SQ SEQUENCE 2177 AA; 245861 MW; D3ABEC0E9A6A7A02 CRC64;
MESIKDLVNV ATGAMDTLSL SSVETEANNI ISGNEVGGEI ITKVADDASN LLGPNSFATT
AQPENKDVVQ ATTTVNTTNL TQHPSAPTIP FTPDFRNVDN FHSMAYDITT GDKNPSKLIR
LDTASWQTSY SRQYKITTVE LPKSFWDDTR KPAYGQAKYF AAVRCGFHFQ VQVNVNQGTA
GSALVVYEPK PVIDSRQYLE FGSLTNLPHV LMNLAETTQA DLCIPYVADT NYVKTDSSDL
GQLRVYVWTP LSVPTGASNE VDVTVMGSLL QLDFQNPRPY GEDVEIYDNG PNKTNIGRFN
KRKFLTASTK YKWTRTKVDI AEGPGTMNMA NVLSTTGAQS VALVGERAFY DPRTAGSKSR
FDDMIKIAQL FSVMSDNTTP SSSSGIDKYG YFDWAATVAP QNMVHRNVVT LDQFPNLNLF
MNTYSYFRGS LILRLSIYAS TFNRGRLRMG FFPNCTHDTQ LELDNAIYTI CDIGSDNSFE
LTIPYSFSTW MRKTHGHQLG LFQVEVLNRL TYNSSSPNKV HCIVQGRLGD DAKFFCPTGS
LVSFQNSWGS QMDLTDPLCI EDNMENCKQS ISPNELGLTS AQDDGPLGNE KPNYFLNFRT
MNVDIFTVSH TRVDNIFGRA WYVTSHDFNN GDTWRQKLTF PKEGHGMLSQ FFAYFTGEIN
IHILYMAEQG FLRVAHTYDT EDNRKTFLSS NGVITIPAGE QMTLSVPFYS NKPLRTVRHD
SALGFLMCRP MMHGTTRTTA EVYISLRCPN FFFPVPAPKP TGSGATALHD ESPYVPTDNR
KMQLAYLDRG FYKHYGIIVG DSVYQLDSDD IFKTALTGKA RFTRTKLTPD WVIEEECELD
YFRVKYLESS VNSEHIFSVD HNCETIAKDI FGTHTLNQHQ AIGLVGTILL TAGLMSTIKT
PVNATTIKEF FNHAVEGDEQ GLSLLVQKCT TFFSTAATEI LDNDLVKFIV KILVRILCYM
VLYCHKPNIL TTACLSTLLI MDVTSSSVLS PSCKALMQCL MDGDVKKLAE VVAESMSNTD
DEEIKEQICD TVKYTKSILS NQGPFKGFNE VSTAFRHIDW WIHTLLKIKD MVLSVFKPSM
ESKAIQWLDR NKEHVCAILD YASDIIVESK DQTKMKSQEF YQKYTDCLSK FKPIMAICFR
SCHNSISNTV YRLFQELARI PSRISTQNDL IRVEPIGIWI QGEPGQGKSF LTHTLSRQLQ
KSCKLNGVYT NPTASEFMDG YDNQDIHLID DLGQTRKEKD IEMLCNCISS VPFIVPMAHL
EEKGKFYTSK LVIATTNKSD FSSTVLQDSG ALKRRFPYIM HIRAAKAYSK AGKLNVSQAM
STMATGECWE VSKNGRDWET LKLKDLVQKI TEDYQERQKN YNCWKQQLEN QTLDDLDDAV
SYIKHNFPDA IPYIDEYLNI EMSILIEQME AFIEPRPSVF KCFATRVANQ TRKAAKEVVE
WFSNKIKSML SFVERNKAWL TVVSAVTSAI SILLLVTKIF KKEDSKDERA YNPTLPVTKP
KGAFPVSQRE FKNEAPYDGQ LEHIISQMAY ITGSTTGHLT HCAGYQHDEI ILHGHSIKYL
EQEEDLTLHY KNKVFPIENP SVTQVTLGGK PMDLAILKCK LPFRFKKNSK YYTNKIGTES
MLIWMTEQGI ITKEVQRVHH SGGIKTREGT ESTKTISYTV KSCKGMCGGL LISKVEGNFK
ILGMHIAGNG EMGVAIPFNF LKNDMSDQGI ITEITPIQPM YINTKTQIHK SPVYGAVEVK
MGPAVLSKSD PRLEEPVECL IKKSAAKYRV NKFQVNNELW QGVKACVKSK FREIFGVNGI
VDMKTAILGT SHVNSMDLST SAGYSFVKSG YKKKDLICLE PFSVSPMLEK LVQDKFHALL
KGNQISTIFN TCLKDELRKL DKISAGKTRC IEACEVDYCI VYRMIMMEIY DKIYQTPCYY
SGLAVGINPY KDWHFMVNAL NDYNYEMDYS QYDGSLSSML LWEAVEVLAY CHDSPDLVMQ
LHKPVIDSDH VVFNERWLIH GGMPSGSPCT TVLNSLCNLM MCIYTTNLIS PGIDCLPIVY
GDDVILSLDK EIDPEKLQSV MADSFGAEVT GSRKDEPPSL KPRLEVEFLK RKPGYFPEST
FIVGKLDTEN MIQHLMWMKN FSTFKQQLQS YLMELCLHGK DTYLHYIKIL DPYLREWNIT
VDDYDVVIAK LMPMVFD
//
