ID U4LUV4_PYROM Unreviewed; 1108 AA.
AC U4LUV4;
DT 11-DEC-2013, integrated into UniProtKB/TrEMBL.
DT 11-DEC-2013, sequence version 1.
DT 27-MAR-2024, entry version 48.
DE RecName: Full=leucine--tRNA ligase {ECO:0000256|ARBA:ARBA00013164};
DE EC=6.1.1.4 {ECO:0000256|ARBA:ARBA00013164};
DE AltName: Full=Leucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00030520};
GN ORFNames=PCON_12053 {ECO:0000313|EMBL:CCX31976.1};
OS Pyronema omphalodes (strain CBS 100304) (Pyronema confluens).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Pezizomycetes;
OC Pezizales; Pyronemataceae; Pyronema.
OX NCBI_TaxID=1076935 {ECO:0000313|EMBL:CCX31976.1};
RN [1] {ECO:0000313|EMBL:CCX31976.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CBS100304 {ECO:0000313|EMBL:CCX31976.1};
RC TISSUE=Vegetative mycelium {ECO:0000313|EMBL:CCX31976.1};
RX PubMed=24068976; DOI=10.1371/journal.pgen.1003820;
RA Traeger S., Altegoer F., Freitag M., Gabaldon T., Kempken F., Kumar A.,
RA Marcet-Houben M., Poggeler S., Stajich J.E., Nowrousian M.;
RT "The genome and development-dependent transcriptomes of Pyronema confluens:
RT a window into fungal evolution.";
RL PLoS Genet. 9:E1003820-E1003820(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00001372};
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DR EMBL; HF935702; CCX31976.1; -; Genomic_DNA.
DR AlphaFoldDB; U4LUV4; -.
DR STRING; 1076935.U4LUV4; -.
DR eggNOG; KOG0437; Eukaryota.
DR OMA; KFIEWQF; -.
DR OrthoDB; 5472610at2759; -.
DR Proteomes; UP000018144; Unassembled WGS sequence.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd07959; Anticodon_Ia_Leu_AEc; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR Gene3D; 3.90.740.10; Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain; 1.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR004493; Leu-tRNA-synth_Ia_arc/euk.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR NCBIfam; TIGR00395; leuS_arch; 1.
DR PANTHER; PTHR45794:SF1; LEUCINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR PANTHER; PTHR45794; LEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 2.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
PE 4: Predicted;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000313|EMBL:CCX31976.1};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917}.
FT DOMAIN 59..125
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 209..783
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 824..938
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
SQ SEQUENCE 1108 AA; 124542 MW; BF87D7C4DE464380 CRC64;
MADALDPANA SKATLKLENT EKRDALIAIE KKYQQFWAEN KTYETDAPTL EEDPITDADE
LREKHPKFFG CMAYPYMNGT LHAGHSFSLS KVEFQTGYSR MQGKRALFPM GFHCTGMPIK
ACADKLKREV ELFGQNFENC PEEDTEEPAA APAKAATKED ITKFAAKKGK AAAKAVKVRW
QFQIMLAIGI PLEDIHKFAD PHHWLGHFPP LCKRDCNSIG LRVDWRRSMI TTDVNPYYDA
FVRWQMNRLR DLGKIKYGER YTIYSPKDGQ PCMDHDRSDG EGVGPQEYTG IKIKVLEWTE
TAQAELAGKL PVGANVYMVA ATLRPETMYG QTCCFVGPTI TYGIYKISET EYYLCTERAA
RNMAYQKIFP KEGEFPCVAT VVGKSLVGTL VKAPLSIHTD GVRVLPMETV LATKGTGVVT
SVPSDSPDDY ATVSDLAKKA EYYGIKKEWA ELEIPEIIQT PSYGNKTAEF LVKKLKINSP
KDAKQLAEAK ELAYKEGFYQ GIMLMGDFKG EKVEVAKPKV RAALIASGEA FAYAEPEGQV
TSRSNDPCIV ALLDQWYLDY GEAQWREQTE NYVKEGLNSY HDETKNGLIA NLGWLNQWAC
ARSFGLGSKL PWDQQFLVES LSDSTIYMAY YTIAHYLHSS IDGKELGLAG ITAEQMVDEV
WDYLFCQREL PQDVIEKNNL PMDWLVKMRR EFEYFYPLDV NISGKDLIQN HLTFFLYIHV
AIFPPKYWPK GIRANGHLML NGEKMSKSTG NFLTLKETVD KFGADAARIA FADAGDSVED
ANFEEKVANA AILRLYTLKE WCEDIVKDAE AGKLRTGPKE TFWDKIFENE MNELVAATKE
NYDSTSYKLA LKTGYYEFQA ARDTYREATS SIGMHKDLVL AWIKTQALMI NPVAPHWAEY
VWQDLLKNTG SIQNERFPVP TAPIDAASTA ARVYVQQTAS NITSTEAAQL KKKAKGKGTS
YDPKLPKQLT IYVSLNYPSW QEKYIDLVRE SFDKVTLTVN DKELSGKVAK LGETKKAMPF
VQGLKKRLQA EDAETVFNRK LAFDEVETLK EVVPTLKKTT GCKNVVVVIV DEGGKTGKTI
DGEVVENLPA LAEGADPGNP RFEFTNIE
//
