ID U4QTD9_9BACT Unreviewed; 304 AA.
AC U4QTD9;
DT 11-DEC-2013, integrated into UniProtKB/TrEMBL.
DT 11-DEC-2013, sequence version 1.
DT 24-JAN-2024, entry version 21.
DE RecName: Full=tRNA-uridine 2-sulfurtransferase {ECO:0000256|ARBA:ARBA00011949};
DE EC=2.8.1.13 {ECO:0000256|ARBA:ARBA00011949};
GN ORFNames=C75L2_00160011 {ECO:0000313|EMBL:EIJ77117.1};
OS Leptospirillum sp. Group II 'C75'.
OC Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
OC Leptospirillum.
OX NCBI_TaxID=1159328 {ECO:0000313|EMBL:EIJ77117.1, ECO:0000313|Proteomes:UP000016760};
RN [1] {ECO:0000313|EMBL:EIJ77117.1, ECO:0000313|Proteomes:UP000016760}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=22539719; DOI=10.1126/science.1218389;
RA Denef V.J., Banfield J.F.;
RT "In situ evolutionary rate measurements show ecological success of recently
RT emerged bacterial hybrids.";
RL Science 336:462-466(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] + uridine(34) in
CC tRNA = 2-thiouridine(34) in tRNA + A + AMP + diphosphate + H(+) + L-
CC cysteinyl-[protein]; Xref=Rhea:RHEA:47032, Rhea:RHEA-COMP:10131,
CC Rhea:RHEA-COMP:11726, Rhea:RHEA-COMP:11727, Rhea:RHEA-COMP:11728,
CC ChEBI:CHEBI:13193, ChEBI:CHEBI:15378, ChEBI:CHEBI:17499,
CC ChEBI:CHEBI:29950, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:61963, ChEBI:CHEBI:65315, ChEBI:CHEBI:87170,
CC ChEBI:CHEBI:456215; EC=2.8.1.13;
CC Evidence={ECO:0000256|ARBA:ARBA00001042};
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DR EMBL; JH660553; EIJ77117.1; -; Genomic_DNA.
DR AlphaFoldDB; U4QTD9; -.
DR HOGENOM; CLU_035188_0_0_0; -.
DR Proteomes; UP000016760; Unassembled WGS sequence.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0016783; F:sulfurtransferase activity; IEA:InterPro.
DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR CDD; cd01998; tRNA_Me_trans; 1.
DR Gene3D; 2.30.30.280; Adenine nucleotide alpha hydrolases-like domains; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR InterPro; IPR023382; MnmA-like_central_sf.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR004506; tRNA-specific_2-thiouridylase.
DR PANTHER; PTHR11933:SF5; MITOCHONDRIAL TRNA-SPECIFIC 2-THIOURIDYLASE 1; 1.
DR PANTHER; PTHR11933; TRNA 5-METHYLAMINOMETHYL-2-THIOURIDYLATE -METHYLTRANSFERASE; 1.
DR Pfam; PF03054; tRNA_Me_trans; 1.
DR SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
PE 4: Predicted;
KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157};
KW Methyltransferase {ECO:0000313|EMBL:EIJ77117.1};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:EIJ77117.1};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694};
KW tRNA-binding {ECO:0000256|ARBA:ARBA00022555}.
SQ SEQUENCE 304 AA; 34699 MW; 83C312FC503D9D0D CRC64;
MKSRVLVGLS GGVDSTVAAL LLKEAGFDVY GAIVEIWSEN SSGGRSGEER EKPWFERACC
HLPMVKFLCE EYLHIPYVQI DQKQNFQEKI VDVFREGYHN GVTPNPCTDC NAEIKIKTLI
DWAEQQKISY VATGHYAKNH YSVQNRLWGI AQALDRKKDQ SYFLSRVPEH VLRKVLFPLG
HWTKDKVREF ARSKKIPVEE MVENMEACFL SAKNVTSFLK REEEVHSSGN WKVTDMKGEK
IGEIPTGIGL TKGQRKGIGV ANAERMYVKK VDVHNKVVIM GSKKDILNRN FRIRDPLGPL
FQKK
//
