ID U4TQY3_9LACO Unreviewed; 572 AA.
AC U4TQY3;
DT 11-DEC-2013, integrated into UniProtKB/TrEMBL.
DT 11-DEC-2013, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE RecName: Full=Rqc2 homolog RqcH {ECO:0000256|HAMAP-Rule:MF_00844};
DE Short=RqcH {ECO:0000256|HAMAP-Rule:MF_00844};
GN Name=yloA {ECO:0000313|EMBL:ERL66644.1};
GN Synonyms=rqcH {ECO:0000256|HAMAP-Rule:MF_00844};
GN ORFNames=L248_0323 {ECO:0000313|EMBL:ERL66644.1};
OS Schleiferilactobacillus shenzhenensis LY-73.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Schleiferilactobacillus.
OX NCBI_TaxID=1231336 {ECO:0000313|EMBL:ERL66644.1, ECO:0000313|Proteomes:UP000030647};
RN [1] {ECO:0000313|Proteomes:UP000030647}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LY-73 {ECO:0000313|Proteomes:UP000030647};
RX PubMed=24265500;
RA Lin Z., Liu Z., Yang R., Zou Y., Wan D., Chen J., Guo M., Zhao J., Fang C.,
RA Yang R., Liu F.;
RT "Whole-Genome Sequencing of Lactobacillus shenzhenensis Strain LY-73T.";
RL Genome Announc. 1:e00972-e00913(2013).
CC -!- FUNCTION: Part of the ribosome quality control system (RQC). Recruits
CC Ala-charged tRNA and directs the elongation of stalled nascent chains
CC on 50S ribosomal subunits, leading to non-templated C-terminal Ala
CC extensions (Ala tail). The Ala tail promotes nascent chain degradation.
CC May add between 1 and at least 8 Ala residues. Binds to stalled 50S
CC ribosomal subunits. {ECO:0000256|HAMAP-Rule:MF_00844}.
CC -!- SUBUNIT: Associates with stalled 50S ribosomal subunits.
CC {ECO:0000256|HAMAP-Rule:MF_00844}.
CC -!- SIMILARITY: Belongs to the NEMF family. {ECO:0000256|HAMAP-
CC Rule:MF_00844}.
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DR EMBL; KI271582; ERL66644.1; -; Genomic_DNA.
DR RefSeq; WP_022528270.1; NZ_KI271582.1.
DR AlphaFoldDB; U4TQY3; -.
DR STRING; 1231336.L248_0323; -.
DR eggNOG; COG1293; Bacteria.
DR HOGENOM; CLU_022481_2_1_9; -.
DR OrthoDB; 9766163at2; -.
DR Proteomes; UP000030647; Unassembled WGS sequence.
DR GO; GO:0043023; F:ribosomal large subunit binding; IEA:UniProtKB-UniRule.
DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0072344; P:rescue of stalled ribosome; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.970.40; fibrinogen binding protein from staphylococcus aureus domain like; 1.
DR Gene3D; 2.30.310.10; ibrinogen binding protein from staphylococcus aureus domain; 1.
DR HAMAP; MF_00844_B; RqcH_B; 1.
DR InterPro; IPR008532; NFACT_RNA-bd.
DR InterPro; IPR043682; RqcH_bacterial.
DR PANTHER; PTHR15239; NUCLEAR EXPORT MEDIATOR FACTOR NEMF; 1.
DR PANTHER; PTHR15239:SF6; RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT NEMF; 1.
DR Pfam; PF05670; NFACT-R_1; 1.
DR Pfam; PF18297; NFACT-R_2; 1.
DR Pfam; PF05833; NFACT_N; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|HAMAP-Rule:MF_00844};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917, ECO:0000256|HAMAP-
KW Rule:MF_00844};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|HAMAP-
KW Rule:MF_00844};
KW tRNA-binding {ECO:0000256|ARBA:ARBA00022555, ECO:0000256|HAMAP-
KW Rule:MF_00844}.
FT DOMAIN 451..539
FT /note="NFACT RNA-binding"
FT /evidence="ECO:0000259|Pfam:PF05670"
FT REGION 166..185
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 430..449
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 290..320
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00844"
FT COILED 377..411
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00844"
SQ SEQUENCE 572 AA; 64454 MW; E6E314AD57A78ED9 CRC64;
MSFDGIFTRG MVNELNRTIV PGKITKIQQP YENEIVLTIR ANRKNYPLLL SAHPTYARIQ
VTDIPYVNPD VPTKFAMTLR KYLDGGVVTG LSQVESDRQV HIHFRSRNEL GDTQELLLIV
ELMGRHSNIM LVEKDGGKIL DLIRHVPPEQ NRYRLLMPGA TYVAPPHEDR RDPLANTPDL
SGVTPSSLDR KLLQSTFQGL GADTANELVA RLQGKPTDQW GFIWNNFFSP VADDQLQPTI
AENDQGKTLF TPFPYSLTTK PVHTAASLSA LLDDYYRDKA QRDRVHQMAS DLLRVVNNNL
NKEKNKLKKQ EQELAATEKA DTYRIKGEVL TSYLHEVKRG MTSVTLPNFY EDGKPLKISL
SNQLSPAGNA QKYFTKYQKL KNAVKYLNTQ IKEAQDEVDY LENILSEIQI ADPGDLGDIK
TELTQQGYLR EKQAKGKKKN RHTPLGKPDK FYASDGTEIL VGKNNLQNDQ LTLHKAAKTD
HWLHVKNIPG SHVIVRSPDP SDQTLTEAAE IAAYYSKARG SGNVPVDTVE VKKVHKPNGA
KPGFVIYEGQ QTLYVTPKES DVLARRRKPE KV
//
