ID U5N3E3_PRORE Unreviewed; 259 AA.
AC U5N3E3;
DT 22-JAN-2014, integrated into UniProtKB/TrEMBL.
DT 22-JAN-2014, sequence version 1.
DT 24-JAN-2024, entry version 41.
DE RecName: Full=Aminoglycoside 3'-phosphotransferase {ECO:0000256|ARBA:ARBA00017903};
DE EC=2.7.1.95 {ECO:0000256|ARBA:ARBA00012193};
GN Name=aphA6 {ECO:0000313|EMBL:AGX85689.1};
GN Synonyms=aphA_2 {ECO:0000313|EMBL:CAB5716263.1};
GN ORFNames=09AY2001NDM_054 {ECO:0000313|EMBL:AGX85689.1}, GHA_04170
GN {ECO:0000313|EMBL:CAB5716263.1};
OS Providencia rettgeri.
OG Plasmid pPrY2001 {ECO:0000313|EMBL:AGX85689.1}.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Morganellaceae; Providencia.
OX NCBI_TaxID=587 {ECO:0000313|EMBL:AGX85689.1};
RN [1] {ECO:0000313|EMBL:AGX85689.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=09ACRGNY2001 {ECO:0000313|EMBL:AGX85689.1};
RC PLASMID=pPrY2001 {ECO:0000313|EMBL:AGX85689.1};
RX PubMed=24275114; DOI=10.1093/jac/dkt445;
RG Canadian Nosocomial Infection Surveillance Program;
RA Mataseje L.F., Boyd D.A., Lefebvre B., Bryce E., Embree J., Gravel D.,
RA Katz K., Kibsey P., Kuhn M., Langley J., Mitchell R., Roscoe D., Simor A.,
RA Taylor G., Thomas E., Turgeon N., Mulvey M.R.;
RT "Complete sequences of a novel blaNDM-1-harbouring plasmid from Providencia
RT rettgeri and an FII-type plasmid from Klebsiella pneumoniae identified in
RT Canada.";
RL J. Antimicrob. Chemother. 69:637-642(2014).
RN [2] {ECO:0000313|EMBL:CAB5716263.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=BB1453 {ECO:0000313|EMBL:CAB5716263.1};
RA Delgado-Blas J.;
RL Submitted (MAY-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + kanamycin A = ADP + H(+) + kanamycin 3'-phosphate;
CC Xref=Rhea:RHEA:24256, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:57909, ChEBI:CHEBI:58214, ChEBI:CHEBI:456216;
CC EC=2.7.1.95; Evidence={ECO:0000256|ARBA:ARBA00001685};
CC -!- SIMILARITY: Belongs to the aminoglycoside phosphotransferase family.
CC {ECO:0000256|ARBA:ARBA00006219, ECO:0000256|PIRNR:PIRNR000706}.
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DR EMBL; KF295828; AGX85689.1; -; Genomic_DNA.
DR EMBL; CAHPSF010000016; CAB5716263.1; -; Genomic_DNA.
DR RefSeq; WP_014386410.1; NZ_VRPH01000050.1.
DR RefSeq; YP_008691665.1; NC_022589.1.
DR AlphaFoldDB; U5N3E3; -.
DR SMR; U5N3E3; -.
DR GeneID; 70094035; -.
DR Proteomes; UP000834611; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0008910; F:kanamycin kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR CDD; cd05150; APH; 1.
DR Gene3D; 3.90.1200.10; -; 1.
DR InterPro; IPR002575; Aminoglycoside_PTrfase.
DR InterPro; IPR024165; Kan/Strep_kinase.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR Pfam; PF01636; APH; 1.
DR PIRSF; PIRSF000706; Kanamycin_kin; 1.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
PE 3: Inferred from homology;
KW Antibiotic resistance {ECO:0000256|ARBA:ARBA00023251,
KW ECO:0000256|PIRNR:PIRNR000706};
KW ATP-binding {ECO:0000256|PIRNR:PIRNR000706};
KW Kinase {ECO:0000256|PIRNR:PIRNR000706};
KW Magnesium {ECO:0000256|PIRSR:PIRSR000706-2};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR000706-2};
KW Nucleotide-binding {ECO:0000256|PIRNR:PIRNR000706};
KW Plasmid {ECO:0000313|EMBL:AGX85689.1};
KW Transferase {ECO:0000256|PIRNR:PIRNR000706, ECO:0000313|EMBL:AGX85689.1}.
FT DOMAIN 17..242
FT /note="Aminoglycoside phosphotransferase"
FT /evidence="ECO:0000259|Pfam:PF01636"
FT ACT_SITE 187
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000706-1"
FT BINDING 192
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR000706-2"
FT BINDING 205
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR000706-2"
SQ SEQUENCE 259 AA; 30277 MW; BE3921E72276EC0B CRC64;
MELPNIIQQF IGNSVLEPNK IGQSPSDVYS FNRNNETFFL KRSSTLYTET TYSVSREAKM
LSWLSEKLKV PELIMTFQDE QFELMITKAI NAKPISALFL TDQELLAIYK EALNLLNSVA
IIDCPFISNI DHRLKESKFF IDNQLLDDID QDDFDAELWG DHRTYLSLWN ELTETRVEER
LVFSHGDITD SNIFIDKFNE IYFLDLGRAG LADEFVDISF VERCLREDAS EETAKIFLKH
LKNDRPDKRN YFLKLDELN
//
