ID   V2GQS6_9BURK            Unreviewed;       705 AA.
AC   V2GQS6;
DT   22-JAN-2014, integrated into UniProtKB/TrEMBL.
DT   22-JAN-2014, sequence version 1.
DT   27-MAR-2024, entry version 44.
DE   RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE            EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN   ORFNames=B551_0219475 {ECO:0000313|EMBL:ESH95848.1};
OS   Cupriavidus sp. HPC(L).
OC   Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Cupriavidus.
OX   NCBI_TaxID=1217418 {ECO:0000313|EMBL:ESH95848.1, ECO:0000313|Proteomes:UP000053474};
RN   [1] {ECO:0000313|EMBL:ESH95848.1, ECO:0000313|Proteomes:UP000053474}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=HPC(L) {ECO:0000313|Proteomes:UP000053474};
RA   Purohit H.J., Agarwal L.;
RT   "Cupriavidus a Desert isolate.";
RL   Submitted (AUG-2013) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC         Evidence={ECO:0000256|ARBA:ARBA00034618};
CC   -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC       {ECO:0000256|ARBA:ARBA00009922}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:ESH95848.1}.
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DR   EMBL; AMPR02000421; ESH95848.1; -; Genomic_DNA.
DR   RefSeq; WP_023264785.1; NZ_AMPR02000421.1.
DR   AlphaFoldDB; V2GQS6; -.
DR   OrthoDB; 5905204at2; -.
DR   Proteomes; UP000053474; Unassembled WGS sequence.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR   CDD; cd17932; DEXQc_UvrD; 1.
DR   Gene3D; 1.10.10.160; -; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR   InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR   InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR   InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014016; UvrD-like_ATP-bd.
DR   PANTHER; PTHR11070:SF3; ATP-DEPENDENT HELICASE_DEOXYRIBONUCLEASE SUBUNIT B; 1.
DR   PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR   Pfam; PF00580; UvrD-helicase; 1.
DR   Pfam; PF13361; UvrD_C; 2.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR   PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW   ProRule:PRU00560};
KW   Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW   ProRule:PRU00560};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW   ProRule:PRU00560};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW   ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000053474}.
FT   DOMAIN          25..324
FT                   /note="UvrD-like helicase ATP-binding"
FT                   /evidence="ECO:0000259|PROSITE:PS51198"
FT   DOMAIN          325..588
FT                   /note="UvrD-like helicase C-terminal"
FT                   /evidence="ECO:0000259|PROSITE:PS51217"
FT   BINDING         46..53
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ   SEQUENCE   705 AA;  78045 MW;  4B4C291083E931D6 CRC64;
     MSVALAPAPA AAPADPADPV DYLAALNPEQ REAVEHPTDQ PLLIIAGAGS GKTNTLAHRV
     AHLLLGGADP RRILLLTFSR RAAVEMGRRV ERIVDQAFAA RNGATSSANP GRAALQWAGT
     FHAIGARLLR EYAETLGLSP SFTISDRGDS ADLINVVRHD LGLSAQNARF PRKETCLAIY
     SRVVNTQLPL EEVLQRQFPR YAMWVDALRT LFAGYVEAKQ KQQVLDYDDL LLYWAQALAE
     PALARDMGER FDHVLVDEYQ DTNALQAAIL LSLKPDGRGL TVVGDDAQSI YAFRGATVRN
     ILDFPHRFSP PAGQVTLSRN YRSTQPILAA ANAVIGLAAE RFTKDLWSDR ASAEKPGVVV
     VNDETEQARF VVERILARRE EGLALMAQAV LFRAADHSAQ LELELVRRNI PFVKFGGLKF
     LESTHVKDML AALRWLENPR DRMAGFRTLQ LMPGVGPKTA ARVLDGLELA SDPLFALESF
     EAPPAAAEAW TELVALAREA MAPAAAWPVD FERVLRWYEP HLERLHDDAA ARAADLQQLA
     RIAATYGSRE RFLTELTLDP PDASSDESGV PLRDEDYLIL STIHSAKGQE WKAVYVLNAV
     DGCLPSDLAT GTTEEIEEER RLLYVAMTRA RDHLDIVVPQ RFYVHQQPGM GDRHVYASRT
     RFLPNRVMPH FHSRAWPPPP PVDAAPQRAS LPAVDLAARM RNMWR
//