ID V3ZCQ5_LOTGI Unreviewed; 499 AA.
AC V3ZCQ5;
DT 22-JAN-2014, integrated into UniProtKB/TrEMBL.
DT 22-JAN-2014, sequence version 1.
DT 27-MAR-2024, entry version 50.
DE RecName: Full=Cytochrome P450 {ECO:0008006|Google:ProtNLM};
GN ORFNames=LOTGIDRAFT_234414 {ECO:0000313|EMBL:ESO88838.1};
OS Lottia gigantea (Giant owl limpet).
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC Patellogastropoda; Lottioidea; Lottiidae; Lottia.
OX NCBI_TaxID=225164 {ECO:0000313|EMBL:ESO88838.1, ECO:0000313|Proteomes:UP000030746};
RN [1] {ECO:0000313|EMBL:ESO88838.1, ECO:0000313|Proteomes:UP000030746}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=23254933; DOI=10.1038/nature11696;
RA Simakov O., Marletaz F., Cho S.J., Edsinger-Gonzales E., Havlak P.,
RA Hellsten U., Kuo D.H., Larsson T., Lv J., Arendt D., Savage R.,
RA Osoegawa K., de Jong P., Grimwood J., Chapman J.A., Shapiro H., Aerts A.,
RA Otillar R.P., Terry A.Y., Boore J.L., Grigoriev I.V., Lindberg D.R.,
RA Seaver E.C., Weisblat D.A., Putnam N.H., Rokhsar D.S.;
RT "Insights into bilaterian evolution from three spiralian genomes.";
RL Nature 493:526-531(2013).
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413;
CC Evidence={ECO:0000256|ARBA:ARBA00001971,
CC ECO:0000256|PIRNR:PIRNR000047, ECO:0000256|PIRSR:PIRSR000047-1};
CC -!- PATHWAY: Lipid metabolism; bile acid biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004860}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000256|PIRNR:PIRNR000047}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family.
CC {ECO:0000256|ARBA:ARBA00010617, ECO:0000256|PIRNR:PIRNR000047}.
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DR EMBL; KB202620; ESO88838.1; -; Genomic_DNA.
DR RefSeq; XP_009060508.1; XM_009062260.1.
DR AlphaFoldDB; V3ZCQ5; -.
DR STRING; 225164.V3ZCQ5; -.
DR EnsemblMetazoa; LotgiT234414; LotgiP234414; LotgiG234414.
DR GeneID; 20249533; -.
DR KEGG; lgi:LOTGIDRAFT_234414; -.
DR CTD; 20249533; -.
DR HOGENOM; CLU_018012_1_3_1; -.
DR OMA; TENHNIW; -.
DR OrthoDB; 1537669at2759; -.
DR Proteomes; UP000030746; Unassembled WGS sequence.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:InterPro.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR CDD; cd11040; CYP7_CYP8-like; 1.
DR Gene3D; 1.10.630.10; Cytochrome P450; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR024204; Cyt_P450_CYP7A1-type.
DR InterPro; IPR002403; Cyt_P450_E_grp-IV.
DR InterPro; IPR036396; Cyt_P450_sf.
DR PANTHER; PTHR24304:SF4; 25-HYDROXYCHOLESTEROL 7-ALPHA-HYDROXYLASE-LIKE; 1.
DR PANTHER; PTHR24304; CYTOCHROME P450 FAMILY 7; 1.
DR Pfam; PF00067; p450; 1.
DR PIRSF; PIRSF000047; Cytochrome_CYPVIIA1; 1.
DR PRINTS; PR00465; EP450IV.
DR SUPFAM; SSF48264; Cytochrome P450; 1.
PE 3: Inferred from homology;
KW Endoplasmic reticulum {ECO:0000256|PIRNR:PIRNR000047};
KW Heme {ECO:0000256|ARBA:ARBA00022617, ECO:0000256|PIRNR:PIRNR000047};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|PIRNR:PIRNR000047};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023221};
KW Membrane {ECO:0000256|PIRNR:PIRNR000047, ECO:0000256|SAM:Phobius};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRNR:PIRNR000047};
KW Reference proteome {ECO:0000313|Proteomes:UP000030746};
KW Steroid metabolism {ECO:0000256|ARBA:ARBA00023221};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 6..24
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT BINDING 111
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000047-2"
FT BINDING 283
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000047-2"
FT BINDING 380
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000047-2"
FT BINDING 429
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000256|PIRSR:PIRSR000047-1"
SQ SEQUENCE 499 AA; 57815 MW; A8496B6AE811EA7A CRC64;
MVAVTVYLAA IAIGLTVAFF KFIYRRRRPN EPEIVPGNLL WGNGAEFAEH AVNFLHRCQK
KFGDIFTIRL LNQYITLVLD PHSYENLARE KVFDFDPIQR QVNHNVFNFE LIDARKMLSE
AGKKVNGRFL TTGMRNFADN LKNAFAKVST VDENGNIYQT IGDNWGQDGL RDLTSKTLFS
ALFYTIFGQG DAIETFEPQT FYKNFDSFHK YFNYLWLGLP VKLFPKATAA LNVLSQQPTS
DEMLKRPGVS DYIKFSTEFM KANNQSEQDI VGHNLVFLHV NYNTFRLAFW VLYHILDDRA
TFMALHKEVQ DLVERKTETA TENGPLGISI EEFDKLPILD SIIRETLRVT SGVFMVRYIT
QDVEFEMDNG DKALMREGDR VAMYPPAIHK DPEIFEDPEI FKHDRFVDAK FYKNGKELKN
PLVAFGSLCP GKKYSLLQTK WFLVNLMNSF DVRLVEGQKT ECDVNYYGHE ILPPTNDVQV
QYRLKEKFQE LDFLARRQC
//
