ID   V3ZLC8_LOTGI            Unreviewed;       526 AA.
AC   V3ZLC8;
DT   22-JAN-2014, integrated into UniProtKB/TrEMBL.
DT   22-JAN-2014, sequence version 1.
DT   27-MAR-2024, entry version 45.
DE   RecName: Full=glutaminase {ECO:0000256|ARBA:ARBA00012918};
DE            EC=3.5.1.2 {ECO:0000256|ARBA:ARBA00012918};
GN   ORFNames=LOTGIDRAFT_133855 {ECO:0000313|EMBL:ESO83195.1};
OS   Lottia gigantea (Giant owl limpet).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Patellogastropoda; Lottioidea; Lottiidae; Lottia.
OX   NCBI_TaxID=225164 {ECO:0000313|EMBL:ESO83195.1, ECO:0000313|Proteomes:UP000030746};
RN   [1] {ECO:0000313|EMBL:ESO83195.1, ECO:0000313|Proteomes:UP000030746}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=23254933; DOI=10.1038/nature11696;
RA   Simakov O., Marletaz F., Cho S.J., Edsinger-Gonzales E., Havlak P.,
RA   Hellsten U., Kuo D.H., Larsson T., Lv J., Arendt D., Savage R.,
RA   Osoegawa K., de Jong P., Grimwood J., Chapman J.A., Shapiro H., Aerts A.,
RA   Otillar R.P., Terry A.Y., Boore J.L., Grigoriev I.V., Lindberg D.R.,
RA   Seaver E.C., Weisblat D.A., Putnam N.H., Rokhsar D.S.;
RT   "Insights into bilaterian evolution from three spiralian genomes.";
RL   Nature 493:526-531(2013).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + L-glutamine = L-glutamate + NH4(+);
CC         Xref=Rhea:RHEA:15889, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:29985, ChEBI:CHEBI:58359; EC=3.5.1.2;
CC         Evidence={ECO:0000256|ARBA:ARBA00001062};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000256|ARBA:ARBA00011881}.
CC   -!- SIMILARITY: Belongs to the glutaminase family.
CC       {ECO:0000256|ARBA:ARBA00011076}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; KB203771; ESO83195.1; -; Genomic_DNA.
DR   RefSeq; XP_009066145.1; XM_009067897.1.
DR   AlphaFoldDB; V3ZLC8; -.
DR   STRING; 225164.V3ZLC8; -.
DR   EnsemblMetazoa; LotgiT133855; LotgiP133855; LotgiG133855.
DR   GeneID; 20233489; -.
DR   KEGG; lgi:LOTGIDRAFT_133855; -.
DR   CTD; 20233489; -.
DR   HOGENOM; CLU_016439_1_0_1; -.
DR   OMA; RWGNRPI; -.
DR   OrthoDB; 537490at2759; -.
DR   Proteomes; UP000030746; Unassembled WGS sequence.
DR   GO; GO:0004359; F:glutaminase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006541; P:glutamine metabolic process; IEA:InterPro.
DR   Gene3D; 1.10.238.210; -; 1.
DR   Gene3D; 1.25.40.20; Ankyrin repeat-containing domain; 1.
DR   Gene3D; 3.40.710.10; DD-peptidase/beta-lactamase superfamily; 1.
DR   HAMAP; MF_00313; Glutaminase; 1.
DR   InterPro; IPR002110; Ankyrin_rpt.
DR   InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR   InterPro; IPR012338; Beta-lactam/transpept-like.
DR   InterPro; IPR015868; Glutaminase.
DR   InterPro; IPR041541; Glutaminase_EF-hand.
DR   NCBIfam; TIGR03814; Gln_ase; 1.
DR   PANTHER; PTHR12544; GLUTAMINASE; 1.
DR   PANTHER; PTHR12544:SF29; GLUTAMINASE; 1.
DR   Pfam; PF12796; Ank_2; 1.
DR   Pfam; PF17959; EF-hand_14; 1.
DR   Pfam; PF04960; Glutaminase; 1.
DR   SMART; SM00248; ANK; 1.
DR   SUPFAM; SSF48403; Ankyrin repeat; 1.
DR   SUPFAM; SSF56601; beta-lactamase/transpeptidase-like; 1.
DR   PROSITE; PS50297; ANK_REP_REGION; 1.
DR   PROSITE; PS50088; ANK_REPEAT; 1.
PE   3: Inferred from homology;
KW   ANK repeat {ECO:0000256|ARBA:ARBA00023043, ECO:0000256|PROSITE-
KW   ProRule:PRU00023}; Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Reference proteome {ECO:0000313|Proteomes:UP000030746};
KW   Repeat {ECO:0000256|ARBA:ARBA00022737}.
FT   DOMAIN          4..92
FT                   /note="Glutaminase EF-hand"
FT                   /evidence="ECO:0000259|Pfam:PF17959"
FT   REPEAT          455..478
FT                   /note="ANK"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00023"
SQ   SEQUENCE   526 AA;  59546 MW;  5E76F1D035B35BEB CRC64;
     MDDIEDRIFD SLCDSEQYLP ILKFKSQLRK AGLRETDPRL AETMKNLAQF QRSVAEMEGV
     HSIHLDRDTF RKCVGDNIIL ISKAFHNNFI IPEFQTFCQS IDDLYWKVKS NTNGKVASYI
     PQLARYSADF WGVSICTIDG QRYSIGDVNI PLCLQSISKP LTYALALNEH SPDLVHEYVG
     HEPSGRQFNE IKLNADDKPH NPMINAGAIV LCSLLKQGSN LADRFDYTSE MFKKLAGNEH
     ISFNNSVFLS ERETADRNFA LAYYLREYKC FPKGTHVMET LDFYFQLCSV EVTCQSGSVI
     AASLANGGIC PITGERILNP YSVRNTLSLM HSCGMYDYSG QFAFDVGLPS KSGVSGGVML
     VVPNVMGIFL WAPPLDIYGN SCKGIQFCEE LVDNFNFHNY DNLRYTQRKQ DPRLQKFTHR
     ANSVVQLLFA AKNGDITALR RCYLSGLDMN LTDYDGRTAL HVASAEGHTE AVIFLLEKCK
     VSPFECDRWD YTALDDAKKF HKTEVVTILE KYMSTNPKQQ DNSQQK
//