ID   V5BZ13_TRYCR            Unreviewed;      1146 AA.
AC   V5BZ13;
DT   22-JAN-2014, integrated into UniProtKB/TrEMBL.
DT   22-JAN-2014, sequence version 1.
DT   27-MAR-2024, entry version 37.
DE   RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE            EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN   ORFNames=TCDM_01510 {ECO:0000313|EMBL:ESS69788.1};
OS   Trypanosoma cruzi Dm28c.
OC   Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC   Trypanosomatida; Trypanosomatidae; Trypanosoma; Schizotrypanum.
OX   NCBI_TaxID=1416333 {ECO:0000313|EMBL:ESS69788.1, ECO:0000313|Proteomes:UP000017861};
RN   [1] {ECO:0000313|EMBL:ESS69788.1, ECO:0000313|Proteomes:UP000017861}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Dm28c {ECO:0000313|EMBL:ESS69788.1,
RC   ECO:0000313|Proteomes:UP000017861};
RX   PubMed=24482508;
RA   Grisard E.C., Teixeira S.M., de Almeida L.G., Stoco P.H., Gerber A.L.,
RA   Talavera-Lopez C., Lima O.C., Andersson B., de Vasconcelos A.T.;
RT   "Trypanosoma cruzi Clone Dm28c Draft Genome Sequence.";
RL   Genome Announc. 2:e01114-13(2014).
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:ESS69788.1}.
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DR   EMBL; AYLP01000009; ESS69788.1; -; Genomic_DNA.
DR   AlphaFoldDB; V5BZ13; -.
DR   EnsemblProtists; ESS69788; ESS69788; TCDM_01510.
DR   VEuPathDB; TriTrypDB:TCDM_01510; -.
DR   Proteomes; UP000017861; Unassembled WGS sequence.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR   GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:InterPro.
DR   GO; GO:0000209; P:protein polyubiquitination; IEA:InterPro.
DR   CDD; cd00078; HECTc; 1.
DR   Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR   Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR   Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR   InterPro; IPR044611; E3A/B/C-like.
DR   InterPro; IPR000569; HECT_dom.
DR   InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR   PANTHER; PTHR45700; UBIQUITIN-PROTEIN LIGASE E3C; 1.
DR   PANTHER; PTHR45700:SF2; UBIQUITIN-PROTEIN LIGASE E3C; 1.
DR   Pfam; PF00632; HECT; 1.
DR   SMART; SM00119; HECTc; 1.
DR   SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR   PROSITE; PS50237; HECT; 1.
PE   4: Predicted;
KW   Ligase {ECO:0000313|EMBL:ESS69788.1}; Membrane {ECO:0000256|SAM:Phobius};
KW   Reference proteome {ECO:0000313|Proteomes:UP000017861};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius};
KW   Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW   ECO:0000256|PROSITE-ProRule:PRU00104}.
FT   TRANSMEM        12..30
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          832..1146
FT                   /note="HECT"
FT                   /evidence="ECO:0000259|PROSITE:PS50237"
FT   ACT_SITE        1114
FT                   /note="Glycyl thioester intermediate"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ   SEQUENCE   1146 AA;  130264 MW;  5BAC9EC9D44D20B0 CRC64;
     MTAFRIRRLY PIYLFISFLR AFLSPFFFLF EDTFVSTPSF FFLLSVSACF PPLPLLTFRR
     LPATGAEEMN GNFVFNGRSR PTHLSLSRDH QPSKDAILAE TRKKRERREI NRVQQQAALR
     IQRKARLWLS RMALADLAFN LFQSIRDESQ KTPGRRLERS CWDFEFFCRG KRRGVDHNSR
     RMMCTQVVLQ QLRRSIRAGS VREELFGCQH GLDLLARLIF EEYARPPVNA EEKETLWKEE
     HLFLFDALLE TNKSLNEKVK LWIRALQLVI QHGTSGNASV VSRIQGLYAS FSACDIAVAM
     EAPEGREMLL EALTSSAHNG PYISPTSCAL DVLLASQHEE ECAVNQEVNR VLLEAIVDVT
     ASKLPEILRF NTTAALGKLV RLTPFFLGAE GSRDGELRRK WLKCLVELTI HASRDDELLK
     TTIVDLMHPH IEGPAHSSRY EHLPTYLFTA DCGLRLLKEN ASAVAALIES SSEGEASSPS
     RSNAFFDFNL LCSTFVWPLY NFSLKSPEQR IEVTTIFSKL VNSSELLRSL WKIYQDCHSS
     TPGDPSGMKK ESLLTRVKAG EVAHLPFTGE PPAELLGTVT GTTSARSLFW DMYPAVSVLF
     FTLLSYFVDA TDFMEGLENG EVMDCNDAVL LLLSLKGIVH RSYMHGIFPH CNGEAVAQTA
     LRLLTKLHVI DEVQSFVPHP SVWIAINDPM ILGTFESITR ETWLDVDAAL EFSKLNYLEG
     NEGDAVQVNS ASICHPHWTG STTWSHQERI VRLLQNAPFT IPFNVRASVF SALILSFEQN
     MLFYSQQPFL VHRGHVFIDA FDRFAKVPDS PDMFSVRFRD TNNLVEEGYG EGVYREFLVN
     LCTEGFAAEY GMFRQTEDGD VYPNPFSYEV TGDPQHLRRI TFLGAMVGRS LRDGVVQDIP
     FAPHFRNAIL GRSNSINNLK SFDRELYRQI MSLRSLSEEE IENLSLTFTY TVDILDEVRE
     VELLRGGRDI PVTRRNCLNY IHLLAGFKLN HESARQTRAF LEGLGMIVNL SWLKLFDGKE
     VMKLFGGDAE SAIDVSDWRK HTQYHRADDE ESAPVHVFWR VVEAMSLEER KRLLKFTTSM
     NRPPLLGFQF LNPPFKVHVE WDASEERLPS ASTCFSTLKL PPYKDFTTAF SKITAAIEGT
     EDFGLT
//