ID   V5EVG1_KALBG            Unreviewed;       598 AA.
AC   V5EVG1;
DT   22-JAN-2014, integrated into UniProtKB/TrEMBL.
DT   22-JAN-2014, sequence version 1.
DT   24-JAN-2024, entry version 40.
DE   RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE            EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN   ORFNames=PSEUBRA_SCAF10g05417 {ECO:0000313|EMBL:EST09465.1};
OS   Kalmanozyma brasiliensis (strain GHG001) (Yeast) (Pseudozyma brasiliensis).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina;
OC   Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Kalmanozyma.
OX   NCBI_TaxID=1365824 {ECO:0000313|EMBL:EST09465.1, ECO:0000313|Proteomes:UP000019377};
RN   [1] {ECO:0000313|Proteomes:UP000019377}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GHG001 {ECO:0000313|Proteomes:UP000019377};
RX   PubMed=24356824; DOI=10.1128/genomea.00920-13;
RA   Oliveira J.V.D.C., dos Santos R.A.C., Borges T.A., Riano-Pachon D.M.,
RA   Goldman G.H.;
RT   "Draft genome sequence of Pseudozyma brasiliensis sp. nov. strain GHG001, a
RT   high producer of endo-1,4-xylanase isolated from an insect pest of
RT   sugarcane.";
RL   Genome Announc. 1:E0092013-E0092013(2013).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC         [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC         cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC         EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
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DR   EMBL; KI545852; EST09465.1; -; Genomic_DNA.
DR   RefSeq; XP_016294454.1; XM_016433987.1.
DR   AlphaFoldDB; V5EVG1; -.
DR   STRING; 1365824.V5EVG1; -.
DR   GeneID; 27416583; -.
DR   eggNOG; KOG0939; Eukaryota.
DR   HOGENOM; CLU_002173_9_4_1; -.
DR   OMA; RTTYQPN; -.
DR   OrthoDB; 164548at2759; -.
DR   Proteomes; UP000019377; Unassembled WGS sequence.
DR   GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR   CDD; cd00078; HECTc; 1.
DR   Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR   Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR   Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR   InterPro; IPR000569; HECT_dom.
DR   InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR   PANTHER; PTHR11254:SF67; E3 UBIQUITIN-PROTEIN LIGASE HUWE1; 1.
DR   PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR   Pfam; PF00632; HECT; 1.
DR   SMART; SM00119; HECTc; 1.
DR   SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR   PROSITE; PS50237; HECT; 1.
PE   4: Predicted;
KW   Reference proteome {ECO:0000313|Proteomes:UP000019377};
KW   Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW   ECO:0000256|PROSITE-ProRule:PRU00104}.
FT   DOMAIN          263..598
FT                   /note="HECT"
FT                   /evidence="ECO:0000259|PROSITE:PS50237"
FT   REGION          47..83
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          137..173
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        59..83
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        157..173
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        565
FT                   /note="Glycyl thioester intermediate"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ   SEQUENCE   598 AA;  68243 MW;  9B2922FD42285597 CRC64;
     MTDPFDPYSL TRVIALDSTG APLRAEARAM QRELGVDPTP EMIRTFMSMP YPGDPRADFR
     HSSSSRSRPS DQTHLEKQKK RREEDEAKLA KIYESFEFDP LWSKLSDVLS RMKNDPGAAQ
     ILLPLIESMM VVSQHVTSPD GETPIVPGDV RPPFSRSSTL GAHSRSSTQG PKTAKEIMED
     NFFAFTEKHR KILNIMVRQN PTLMSGSFAL LVRNPKVLDF DNKKNYFTQQ LHKGRRDHYT
     PLSLSVRRNS VFEDSFRYFS RKSGPEVKHG KLNVRFNNEE GIDAGGVTRE WFQVLARAMF
     NPDYALFQPC AADRTTYQPN RMSYVNPDHL SFFKFVGRII GKAIYDGRLL DAYFTRSFYK
     HILGKPVDYR DLESIDPEYF KSLEWMLNND ITDILDLTFS VDDEEFGETK IVELQPNGAS
     IAVTEANKQE YVRLVTEQRL TKSIKSQIEA FLGGFNEIIP SDLIRIFSEQ ELELLISGLP
     DIDVDAWKNN TELHGYSSGD AVIQWWWRAV RSFDQTEKAK LLQFITGTSK VPLEGFAHLQ
     GVQGTQRFNI HKAYGADRLP AAHTCFNQLD LPQYESYEKL RSSLLLAMNE GGEGFGFA
//