ID V5RHA5_SPIAP Unreviewed; 478 AA.
AC V5RHA5;
DT 19-FEB-2014, integrated into UniProtKB/TrEMBL.
DT 19-FEB-2014, sequence version 1.
DT 27-MAR-2024, entry version 43.
DE RecName: Full=Pyruvate kinase {ECO:0000256|ARBA:ARBA00012142, ECO:0000256|RuleBase:RU000504};
DE EC=2.7.1.40 {ECO:0000256|ARBA:ARBA00012142, ECO:0000256|RuleBase:RU000504};
GN Name=pyk2 {ECO:0000313|EMBL:AHB36037.1};
GN ORFNames=SAPIS_v1c01910 {ECO:0000313|EMBL:AHB36037.1};
OS Spiroplasma apis B31.
OC Bacteria; Mycoplasmatota; Mollicutes; Entomoplasmatales; Spiroplasmataceae;
OC Spiroplasma.
OX NCBI_TaxID=1276258 {ECO:0000313|EMBL:AHB36037.1, ECO:0000313|Proteomes:UP000018550};
RN [1] {ECO:0000313|EMBL:AHB36037.1, ECO:0000313|Proteomes:UP000018550}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=B31 {ECO:0000313|EMBL:AHB36037.1};
RX PubMed=24407648;
RA Ku C., Lo W.S., Chen L.L., Kuo C.H.;
RT "Complete Genome Sequence of Spiroplasma apis B31T (ATCC 33834), a
RT Bacterium Associated with May Disease of Honeybees (Apis mellifera).";
RL Genome Announc. 2:e01151-13(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + pyruvate = ADP + H(+) + phosphoenolpyruvate;
CC Xref=Rhea:RHEA:18157, ChEBI:CHEBI:15361, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:58702, ChEBI:CHEBI:456216;
CC EC=2.7.1.40; Evidence={ECO:0000256|RuleBase:RU000504};
CC -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC glyceraldehyde 3-phosphate: step 5/5. {ECO:0000256|ARBA:ARBA00004997,
CC ECO:0000256|RuleBase:RU000504}.
CC -!- SUBUNIT: Homotetramer. {ECO:0000256|ARBA:ARBA00011881}.
CC -!- SIMILARITY: Belongs to the pyruvate kinase family.
CC {ECO:0000256|ARBA:ARBA00008663, ECO:0000256|RuleBase:RU000504}.
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DR EMBL; CP006682; AHB36037.1; -; Genomic_DNA.
DR RefSeq; WP_023788971.1; NC_022998.1.
DR AlphaFoldDB; V5RHA5; -.
DR STRING; 1276258.SAPIS_v1c01910; -.
DR KEGG; sapi:SAPIS_v1c01910; -.
DR PATRIC; fig|1276258.3.peg.184; -.
DR eggNOG; COG0469; Bacteria.
DR HOGENOM; CLU_015439_0_1_14; -.
DR OrthoDB; 9812123at2; -.
DR UniPathway; UPA00109; UER00188.
DR Proteomes; UP000018550; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0030955; F:potassium ion binding; IEA:InterPro.
DR GO; GO:0004743; F:pyruvate kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR Gene3D; 3.20.20.60; Phosphoenolpyruvate-binding domains; 1.
DR Gene3D; 2.40.33.10; PK beta-barrel domain-like; 1.
DR Gene3D; 3.40.1380.20; Pyruvate kinase, C-terminal domain; 1.
DR InterPro; IPR001697; Pyr_Knase.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR InterPro; IPR040442; Pyrv_Kinase-like_dom_sf.
DR InterPro; IPR011037; Pyrv_Knase-like_insert_dom_sf.
DR InterPro; IPR015793; Pyrv_Knase_brl.
DR InterPro; IPR036918; Pyrv_Knase_C_sf.
DR InterPro; IPR015806; Pyrv_Knase_insert_dom_sf.
DR NCBIfam; TIGR01064; pyruv_kin; 1.
DR PANTHER; PTHR11817; PYRUVATE KINASE; 1.
DR PANTHER; PTHR11817:SF132; PYRUVATE KINASE 1; 1.
DR Pfam; PF00224; PK; 1.
DR PRINTS; PR01050; PYRUVTKNASE.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR SUPFAM; SSF50800; PK beta-barrel domain-like; 1.
DR SUPFAM; SSF52935; PK C-terminal domain-like; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Glycolysis {ECO:0000256|ARBA:ARBA00023152, ECO:0000256|RuleBase:RU000504};
KW Kinase {ECO:0000256|ARBA:ARBA00022777, ECO:0000256|RuleBase:RU000504};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|RuleBase:RU000504};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Pyruvate {ECO:0000256|ARBA:ARBA00023317, ECO:0000313|EMBL:AHB36037.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000018550};
KW Transferase {ECO:0000256|RuleBase:RU000504}.
FT DOMAIN 10..333
FT /note="Pyruvate kinase barrel"
FT /evidence="ECO:0000259|Pfam:PF00224"
SQ SEQUENCE 478 AA; 54143 MW; 4708DB30C5C79C25 CRC64;
MKIYNLKDKM KRTKVITTVG PSVHTKEAIK ELYDKGMTTI RLNFSHADFS EHGERFEWVK
ELRKEHKLPI SILLDTKGPE IRVGKMKDGK QEIKAGTEVT VYTNPEDFKT RECGPTELQM
SYDMSQDVKV GDVVLVDDGK LTMHVTDVKP GLVMCKAFNN HVVKTNKRVN LPGVDFTLPF
LAEKDYNDIK FGIENNIDYI AASFVNTADN VKEIRNILKE NNAEHIQIIS KIESQIGIDN
IDSIIDASDG IMIARGDLGL EIPYYEVPFQ EKRIIRKCRE KGKLVIVATQ MLESMTDNPQ
PTRAEVTDVY YATELGSDAT MLSGESANGD YPFITTETMS TINKRAEIEF YTKLYYEKQL
ENARNTTSGK RADIANQLAN TTMDGKYEYA VVASRTGELL KTISKFRPNV TILGVTDDEK
LWTSFGVWHS IFMSKVENFD DFLNDEKAVS EIAKSWGAKS GEKILFVRNE NIKEISVL
//
