ID V6KF10_STRNV Unreviewed; 615 AA.
AC V6KF10;
DT 19-FEB-2014, integrated into UniProtKB/TrEMBL.
DT 19-FEB-2014, sequence version 1.
DT 24-JAN-2024, entry version 31.
DE SubName: Full=Glucosylceramidase {ECO:0000313|EMBL:EST30046.1};
GN ORFNames=M877_10830 {ECO:0000313|EMBL:EST30046.1};
OS Streptomyces niveus NCIMB 11891.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1352941 {ECO:0000313|EMBL:EST30046.1, ECO:0000313|Proteomes:UP000017971};
RN [1] {ECO:0000313|EMBL:EST30046.1, ECO:0000313|Proteomes:UP000017971}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCIMB 11891 {ECO:0000313|EMBL:EST30046.1,
RC ECO:0000313|Proteomes:UP000017971};
RX PubMed=24407644;
RA Flinspach K., Ruckert C., Kalinowski J., Heide L., Apel A.K.;
RT "Draft Genome Sequence of Streptomyces niveus NCIMB 11891, Producer of the
RT Aminocoumarin Antibiotic Novobiocin.";
RL Genome Announc. 2:e01146-e01113(2014).
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 30 family.
CC {ECO:0000256|ARBA:ARBA00005382, ECO:0000256|RuleBase:RU361188}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EST30046.1}.
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DR EMBL; AWQW01000076; EST30046.1; -; Genomic_DNA.
DR AlphaFoldDB; V6KF10; -.
DR STRING; 193462.BBN63_09080; -.
DR PATRIC; fig|1352941.4.peg.2217; -.
DR eggNOG; COG5520; Bacteria.
DR HOGENOM; CLU_014379_3_0_11; -.
DR Proteomes; UP000017971; Chromosome.
DR GO; GO:0004348; F:glucosylceramidase activity; IEA:InterPro.
DR GO; GO:0006665; P:sphingolipid metabolic process; IEA:InterPro.
DR CDD; cd00161; RICIN; 1.
DR Gene3D; 2.80.10.50; -; 2.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR033452; GH30_C.
DR InterPro; IPR001139; Glyco_hydro_30.
DR InterPro; IPR033453; Glyco_hydro_30_TIM-barrel.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR035992; Ricin_B-like_lectins.
DR InterPro; IPR000772; Ricin_B_lectin.
DR PANTHER; PTHR11069; GLUCOSYLCERAMIDASE; 1.
DR PANTHER; PTHR11069:SF23; LYSOSOMAL ACID GLUCOSYLCERAMIDASE; 1.
DR Pfam; PF02055; Glyco_hydro_30; 1.
DR Pfam; PF17189; Glyco_hydro_30C; 1.
DR Pfam; PF00652; Ricin_B_lectin; 1.
DR SMART; SM00458; RICIN; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF50370; Ricin B-like lectins; 1.
DR PROSITE; PS50231; RICIN_B_LECTIN; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|RuleBase:RU361188};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361188};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..22
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 23..615
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5004748186"
FT DOMAIN 486..614
FT /note="Ricin B lectin"
FT /evidence="ECO:0000259|SMART:SM00458"
SQ SEQUENCE 615 AA; 65165 MW; DCC0C6296ED978AD CRC64;
MLLVGALTTA GLTGVSAGTA QAAGEQVNIW LTTTSDGGGR TVTRGLQQQA PVNFQPGTGG
SGTNVTVNEN TKYQTFTGGG ASFTDTAAWL MKGSGALSQA TRDDTMRKLF SPTDGIGLSF
IRNPLGGSDL ARFGYTFDDM PAGQTDPDLS EFSIAHDLQD VLPLTKQAKQ LNPALTTMAS
PWTAPAWMKD NGQLNGGWLK AENYGVYADY FVKYLQAYRD QGLPIDYVSA QNEPTCCSSY
PSMSWNASGL AYFTKAELLP GLQAAGLSTK VMAHDWNWDT YDAYAAETVN DPAIRSHPNF
GGIAWHGYGG DVGKQTQVHN QYPQLDAFQT EHSGGTWIGN QQTEDMSNII NYTRNWAKSV
TKWSLAVDQN RGPHNGGCGT CDGLITVNNG GAQHGQVKYN IEYYTMGHLT KFVTPGAHRI
ESSASTTVPN VAWQNPNGSK ALIAYNEASQ AREIRVNWGG QNFSYSLPGK TSATFTWAGG
QSGGGGGTTG SLNGLGGKCL EVAGGNSANE TAVQLWDCNG SAAQRWTIGG DRAVRALGKC
LDVAGGSKAN GAKVQLYDCN GSGAQQWRYE SASGDLVNAP ADKCLDITGN SSANGAKAQL
WSCTGAANQK WRLQT
//
