ID V6LGB1_9EUKA Unreviewed; 425 AA.
AC V6LGB1;
DT 19-FEB-2014, integrated into UniProtKB/TrEMBL.
DT 19-FEB-2014, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE RecName: Full=Exonuclease 1 {ECO:0000256|RuleBase:RU910737};
DE EC=3.1.-.- {ECO:0000256|RuleBase:RU910737};
GN ORFNames=SS50377_17729 {ECO:0000313|EMBL:EST42706.1}, SS50377_25692
GN {ECO:0000313|EMBL:KAH0571506.1};
OS Spironucleus salmonicida.
OC Eukaryota; Metamonada; Diplomonadida; Hexamitidae; Hexamitinae;
OC Spironucleus.
OX NCBI_TaxID=348837 {ECO:0000313|EMBL:EST42706.1};
RN [1] {ECO:0000313|EMBL:KAH0571506.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 50377 {ECO:0000313|EMBL:KAH0571506.1};
RX PubMed=24516394;
RA Xu F., Jerlstrom-Hultqvist J., Einarsson E., Astvaldsson A., Svard S.G.,
RA Andersson J.O.;
RT "The Genome of Spironucleus salmonicida Highlights a Fish Pathogen Adapted
RT to Fluctuating Environments.";
RL PLoS Genet. 10:E1004053-E1004053(2014).
RN [2] {ECO:0000313|EMBL:EST42706.1}
RP NUCLEOTIDE SEQUENCE.
RA Xu F., Jerlstrom-Hultqvist J., Einarsson E., Astvaldsson A., Svard S.G.,
RA Andersson J.O.;
RT "The Genome of Spironucleus salmonicida Highlights a Fish Pathogen Adapted
RT to Fluctuating Environments.";
RL PLoS Genet. 0:0-0(2014).
RN [3] {ECO:0000313|EMBL:KAH0571506.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 50377 {ECO:0000313|EMBL:KAH0571506.1};
RA Xu F., Kurt Z., Jimenez-Gonzalez A., Astvaldsson A., Andersson J.O.,
RA Svard S.G.;
RT "New Spironucleus salmonicida genome in near-complete chromosomes.";
RL Submitted (DEC-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: 5'->3' double-stranded DNA exonuclease which may also possess
CC a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in
CC DNA mismatch repair. {ECO:0000256|RuleBase:RU910737}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|RuleBase:RU910737};
CC Note=Binds 2 magnesium ions per subunit. They probably participate in
CC the reaction catalyzed by the enzyme. May bind an additional third
CC magnesium ion after substrate binding. {ECO:0000256|RuleBase:RU910737};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|RuleBase:RU910737}.
CC -!- SIMILARITY: Belongs to the XPG/RAD2 endonuclease family. EXO1
CC subfamily. {ECO:0000256|RuleBase:RU910737}.
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DR EMBL; KI546157; EST42706.1; -; Genomic_DNA.
DR EMBL; AUWU02000006; KAH0571506.1; -; Genomic_DNA.
DR AlphaFoldDB; V6LGB1; -.
DR EnsemblProtists; EST42706; EST42706; SS50377_17729.
DR VEuPathDB; GiardiaDB:SS50377_25692; -.
DR OrthoDB; 126305at2759; -.
DR Proteomes; UP000018208; Unassembled WGS sequence.
DR GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR006086; XPG-I_dom.
DR InterPro; IPR006084; XPG/Rad2.
DR InterPro; IPR006085; XPG_DNA_repair_N.
DR PANTHER; PTHR11081:SF8; EXONUCLEASE 1; 1.
DR PANTHER; PTHR11081; FLAP ENDONUCLEASE FAMILY MEMBER; 1.
DR Pfam; PF00867; XPG_I; 1.
DR Pfam; PF00752; XPG_N; 1.
DR PRINTS; PR00853; XPGRADSUPER.
DR SMART; SM00484; XPGI; 1.
DR SMART; SM00485; XPGN; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|RuleBase:RU910737};
KW DNA excision {ECO:0000256|RuleBase:RU910737};
KW DNA repair {ECO:0000256|RuleBase:RU910737};
KW DNA-binding {ECO:0000256|RuleBase:RU910737};
KW Excision nuclease {ECO:0000256|RuleBase:RU910737};
KW Exonuclease {ECO:0000256|RuleBase:RU910737, ECO:0000313|EMBL:EST42706.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU910737};
KW Magnesium {ECO:0000256|RuleBase:RU910737};
KW Metal-binding {ECO:0000256|RuleBase:RU910737};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722, ECO:0000256|RuleBase:RU910737};
KW Nucleus {ECO:0000256|RuleBase:RU910737};
KW Reference proteome {ECO:0000313|Proteomes:UP000018208}.
FT DOMAIN 1..97
FT /note="XPG N-terminal"
FT /evidence="ECO:0000259|SMART:SM00485"
FT DOMAIN 142..219
FT /note="XPG-I"
FT /evidence="ECO:0000259|SMART:SM00484"
SQ SEQUENCE 425 AA; 48416 MW; 5F0D88FDF56A6400 CRC64;
MGITGLNENM TGQQTSLKSL QNKSIGIDGH CLLHKAAQNF SQQLLIHKSV IEIANQTYKY
LLQFVKYSKY TILVFDGGYL PAKRPTNEAR KINRDRSLKE AISSSGSHQT KLLQKSISFT
FEQVKDVAEF LISKFQNEQF YCFLSPFEAD AQLAFLNRTN LADVIITLDS DIYLYGVDQV
LFNYNFGLNS GKLVLNCENT LFTEVQQLKN CSILSGCDYL GSLKGVGLKK AQQAILQGQT
ALAGSEILKK GKNRDFPEGI VNMSQYQQRI LEALVVFQYQ RVYNPIDNNI QMLSDCTQDQ
ILLDKFGDII GEQDSLFNVF RNSKIIQDSC QQLRIDLKHN FIHIGNNLQI IEAEKLNVDA
LKLHIIQSDR LETQYQLSVK IQQVQKPEIV SKYHTNLSNN NQINYDSIQF ETQIYTSILD
AKFLQ
//
