ID V6LGT0_9EUKA Unreviewed; 435 AA.
AC V6LGT0;
DT 19-FEB-2014, integrated into UniProtKB/TrEMBL.
DT 19-FEB-2014, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE RecName: Full=Exonuclease 1 {ECO:0000256|RuleBase:RU910737};
DE EC=3.1.-.- {ECO:0000256|RuleBase:RU910737};
GN ORFNames=SS50377_16485 {ECO:0000313|EMBL:EST43750.1}, SS50377_27122
GN {ECO:0000313|EMBL:KAH0570830.1};
OS Spironucleus salmonicida.
OC Eukaryota; Metamonada; Diplomonadida; Hexamitidae; Hexamitinae;
OC Spironucleus.
OX NCBI_TaxID=348837 {ECO:0000313|EMBL:EST43750.1};
RN [1] {ECO:0000313|EMBL:KAH0570830.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 50377 {ECO:0000313|EMBL:KAH0570830.1};
RX PubMed=24516394;
RA Xu F., Jerlstrom-Hultqvist J., Einarsson E., Astvaldsson A., Svard S.G.,
RA Andersson J.O.;
RT "The Genome of Spironucleus salmonicida Highlights a Fish Pathogen Adapted
RT to Fluctuating Environments.";
RL PLoS Genet. 10:E1004053-E1004053(2014).
RN [2] {ECO:0000313|EMBL:EST43750.1}
RP NUCLEOTIDE SEQUENCE.
RA Xu F., Jerlstrom-Hultqvist J., Einarsson E., Astvaldsson A., Svard S.G.,
RA Andersson J.O.;
RT "The Genome of Spironucleus salmonicida Highlights a Fish Pathogen Adapted
RT to Fluctuating Environments.";
RL PLoS Genet. 0:0-0(2014).
RN [3] {ECO:0000313|EMBL:KAH0570830.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 50377 {ECO:0000313|EMBL:KAH0570830.1};
RA Xu F., Kurt Z., Jimenez-Gonzalez A., Astvaldsson A., Andersson J.O.,
RA Svard S.G.;
RT "New Spironucleus salmonicida genome in near-complete chromosomes.";
RL Submitted (DEC-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: 5'->3' double-stranded DNA exonuclease which may also possess
CC a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in
CC DNA mismatch repair. {ECO:0000256|RuleBase:RU910737}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|RuleBase:RU910737};
CC Note=Binds 2 magnesium ions per subunit. They probably participate in
CC the reaction catalyzed by the enzyme. May bind an additional third
CC magnesium ion after substrate binding. {ECO:0000256|RuleBase:RU910737};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|RuleBase:RU910737}.
CC -!- SIMILARITY: Belongs to the XPG/RAD2 endonuclease family. EXO1
CC subfamily. {ECO:0000256|RuleBase:RU910737}.
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DR EMBL; KI546134; EST43750.1; -; Genomic_DNA.
DR EMBL; AUWU02000007; KAH0570830.1; -; Genomic_DNA.
DR AlphaFoldDB; V6LGT0; -.
DR EnsemblProtists; EST43750; EST43750; SS50377_16485.
DR VEuPathDB; GiardiaDB:SS50377_27122; -.
DR OrthoDB; 126305at2759; -.
DR Proteomes; UP000018208; Unassembled WGS sequence.
DR GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR006086; XPG-I_dom.
DR InterPro; IPR006084; XPG/Rad2.
DR PANTHER; PTHR11081:SF8; EXONUCLEASE 1; 1.
DR PANTHER; PTHR11081; FLAP ENDONUCLEASE FAMILY MEMBER; 1.
DR Pfam; PF00867; XPG_I; 1.
DR PRINTS; PR00853; XPGRADSUPER.
DR SUPFAM; SSF88723; PIN domain-like; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|RuleBase:RU910737};
KW DNA excision {ECO:0000256|RuleBase:RU910737};
KW DNA repair {ECO:0000256|RuleBase:RU910737};
KW DNA-binding {ECO:0000256|RuleBase:RU910737};
KW Excision nuclease {ECO:0000256|RuleBase:RU910737};
KW Exonuclease {ECO:0000256|RuleBase:RU910737, ECO:0000313|EMBL:EST43750.1};
KW Hydrolase {ECO:0000256|RuleBase:RU910737};
KW Magnesium {ECO:0000256|RuleBase:RU910737};
KW Metal-binding {ECO:0000256|RuleBase:RU910737};
KW Nuclease {ECO:0000256|RuleBase:RU910737};
KW Nucleus {ECO:0000256|RuleBase:RU910737};
KW Reference proteome {ECO:0000313|Proteomes:UP000018208}.
FT DOMAIN 142..221
FT /note="XPG-I"
FT /evidence="ECO:0000259|Pfam:PF00867"
SQ SEQUENCE 435 AA; 50652 MW; FE429A22D0F1518E CRC64;
MGIKKLTGLL PQTPIPYGYF INKTIAIDFY NFAFISAPYT GYDLIVRDNV QSFYIFFNSL
IKKIMKYRNA KVIICQDGQL LAGKAPVSLP RMQKREKIRK SITKNTKRKR AQKLTSKGFN
ITKLQCQKVF ELLKKDFPED RFSVICAPYE ADFQIDYFVK SGMIDCVLSN DSDMPLLGVD
VITHFGSKSQ MLISKTDMEM FISKYGGMDS MFKCCLLMGN DYYPGHNKPM DDIYQQLLDN
NFDLLKTAFA LNQNNSRYSD GQLVKTLFLA YAIQYYPCIF DFSKKQITHL RQISKEDQEV
FRTCFGKDIV GKESSDEKII MQISGYALNS SKKIKSLQFF KNIFENSLKK MYRQPKQFNL
TDFSILNILK CVKVEGTIYR YEDKQFDLSE PALYAHYLFQ QSIQEQLQFN FNEKIPENYI
HIDLSTEIDK ESSME
//
