ID V6TIQ8_GIAIN Unreviewed; 621 AA.
AC V6TIQ8;
DT 19-FEB-2014, integrated into UniProtKB/TrEMBL.
DT 19-FEB-2014, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE RecName: Full=FAD synthase {ECO:0000256|ARBA:ARBA00012393};
DE EC=2.7.7.2 {ECO:0000256|ARBA:ARBA00012393};
DE AltName: Full=FAD pyrophosphorylase {ECO:0000256|ARBA:ARBA00031145};
DE AltName: Full=FMN adenylyltransferase {ECO:0000256|ARBA:ARBA00031871};
GN ORFNames=DHA2_150166 {ECO:0000313|EMBL:ESU38863.1};
OS Giardia intestinalis (Giardia lamblia).
OC Eukaryota; Metamonada; Diplomonadida; Hexamitidae; Giardiinae; Giardia.
OX NCBI_TaxID=5741 {ECO:0000313|EMBL:ESU38863.1, ECO:0000313|Proteomes:UP000018320};
RN [1] {ECO:0000313|Proteomes:UP000018320}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=DH {ECO:0000313|Proteomes:UP000018320};
RA Adam R., Dahlstrom E., Martens C., Bruno D., Barbian K., Porcella S.F.,
RA Nash T.;
RT "Genome sequencing of Giardia lamblia Genotypes A2 and B isolates (DH and
RT GS) and comparative analysis with the genomes of Genotypes A1 and E (WB and
RT Pig).";
RL Submitted (FEB-2012) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ESU38863.1, ECO:0000313|Proteomes:UP000018320}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DH {ECO:0000313|EMBL:ESU38863.1,
RC ECO:0000313|Proteomes:UP000018320};
RX PubMed=24307482; DOI=10.1093/gbe/evt197;
RA Adam R.D., Dahlstrom E.W., Martens C.A., Bruno D.P., Barbian K.D.,
RA Ricklefs S.M., Hernandez M.M., Narla N.P., Patel R.B., Porcella S.F.,
RA Nash T.E.;
RT "Genome sequencing of Giardia lamblia genotypes A2 and B isolates (DH and
RT GS) and comparative analysis with the genomes of genotypes A1 and E (WB and
RT Pig).";
RL Genome Biol. Evol. 5:2498-2511(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + FMN + H(+) = diphosphate + FAD; Xref=Rhea:RHEA:17237,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:57692, ChEBI:CHEBI:58210; EC=2.7.7.2;
CC Evidence={ECO:0000256|ARBA:ARBA00001332};
CC -!- PATHWAY: Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step
CC 1/1. {ECO:0000256|ARBA:ARBA00004726}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ESU38863.1}.
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DR EMBL; AHGT01000008; ESU38863.1; -; Genomic_DNA.
DR AlphaFoldDB; V6TIQ8; -.
DR EnsemblProtists; ESU38863; ESU38863; DHA2_150166.
DR VEuPathDB; GiardiaDB:DHA2_150166; -.
DR VEuPathDB; GiardiaDB:GL50581_818; -.
DR VEuPathDB; GiardiaDB:GL50803_0016269; -.
DR VEuPathDB; GiardiaDB:QR46_0418; -.
DR Proteomes; UP000018320; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR CDD; cd01713; PAPS_reductase; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR InterPro; IPR002500; PAPS_reduct.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR PANTHER; PTHR23293:SF9; FAD SYNTHASE; 1.
DR PANTHER; PTHR23293; FAD SYNTHETASE-RELATED FMN ADENYLYLTRANSFERASE; 1.
DR Pfam; PF01507; PAPS_reduct; 1.
DR SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW FAD {ECO:0000256|ARBA:ARBA00022827};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 231..308
FT /note="Phosphoadenosine phosphosulphate reductase"
FT /evidence="ECO:0000259|Pfam:PF01507"
FT REGION 52..109
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 364..397
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 52..83
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 621 AA; 69526 MW; 341B1B6A754D88AE CRC64;
MSTHHTYPEF LFSPPRKEEA ILLNPDSVIP KGSEWFPECK SILEFAQKAA NFTNGPTGDQ
ESPQSATISA SNNPRQTSDL NPVRPPNEIR HMADNNDVRL NPSSTESTET PLVNDSLELQ
FAQKIYQALV VIRVAMRQYR RLAFSFNGGK DNTVVLYLIR AACLQAVIEE NSDFESITPH
ELLRSRFIFF YVHNEVQIKQ VMKFMCLIDH EQNLGTVVYL GTSFKNCIVN FYNDYHSDVV
FMGVRSTDPN GKTTIFSHCS PSWPQFMRVC PILYWSYVDV WDFLKRFRIH YCTLYDQGYT
SLGSATHTIK HPTLRDGAIM HRRIASMVHE PQFSAIGGST QTGKQTLQQI THIFTSRVSL
STYGSNQESH TARDKNNQHN KNDIPVSNGR RTPIMPQVEW PTDQAPSAVK ERENEHPCSK
TLLLTDYLSR GTMSVPSVKG TDGSTETPAS TKLRAMTSTS VASLVSTTDE EIEQGYPSMV
TPSLVVDSSN SSMISQANIL HDRNMHQILA GRETHRHHRL LSNTDAALSA SEEYSEAGSI
TSYMGDAGNE YYGSVTPQDV CAYSGPRLVI DELVARGVPT ELLPPQFDPS EEDGEIEDDL
CSASAIFLPV SEGEDERINR V
//
