ID V6U239_GIAIN Unreviewed; 791 AA.
AC V6U239;
DT 19-FEB-2014, integrated into UniProtKB/TrEMBL.
DT 19-FEB-2014, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE RecName: Full=ATP-dependent RNA helicase {ECO:0000256|RuleBase:RU365068};
DE EC=3.6.4.13 {ECO:0000256|RuleBase:RU365068};
GN ORFNames=GSB_13220 {ECO:0000313|EMBL:ESU44662.1};
OS Giardia intestinalis (Giardia lamblia).
OC Eukaryota; Metamonada; Diplomonadida; Hexamitidae; Giardiinae; Giardia.
OX NCBI_TaxID=5741 {ECO:0000313|EMBL:ESU44662.1, ECO:0000313|Proteomes:UP000018040};
RN [1] {ECO:0000313|Proteomes:UP000018040}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=GS {ECO:0000313|Proteomes:UP000018040};
RA Adam R., Dahlstrom E., Martens C., Bruno D., Barbian K., Porcella S.F.,
RA Nash T.;
RT "Genome sequencing of Giardia lamblia Genotypes A2 and B isolates (DH and
RT GS) and comparative analysis with the genomes of Genotypes A1 and E (WB and
RT Pig).";
RL Submitted (FEB-2012) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ESU44662.1, ECO:0000313|Proteomes:UP000018040}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GS {ECO:0000313|EMBL:ESU44662.1,
RC ECO:0000313|Proteomes:UP000018040};
RX PubMed=24307482; DOI=10.1093/gbe/evt197;
RA Adam R.D., Dahlstrom E.W., Martens C.A., Bruno D.P., Barbian K.D.,
RA Ricklefs S.M., Hernandez M.M., Narla N.P., Patel R.B., Porcella S.F.,
RA Nash T.E.;
RT "Genome sequencing of Giardia lamblia genotypes A2 and B isolates (DH and
RT GS) and comparative analysis with the genomes of genotypes A1 and E (WB and
RT Pig).";
RL Genome Biol. Evol. 5:2498-2511(2013).
CC -!- FUNCTION: RNA helicase. {ECO:0000256|RuleBase:RU365068}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000256|ARBA:ARBA00001556,
CC ECO:0000256|RuleBase:RU365068};
CC -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC family of RNA helicases and controls ATP binding and hydrolysis.
CC {ECO:0000256|RuleBase:RU365068}.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family.
CC {ECO:0000256|RuleBase:RU365068}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ESU44662.1}.
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DR EMBL; AHHH01000018; ESU44662.1; -; Genomic_DNA.
DR AlphaFoldDB; V6U239; -.
DR EnsemblProtists; ESU44662; ESU44662; GSB_13220.
DR VEuPathDB; GiardiaDB:DHA2_13220; -.
DR VEuPathDB; GiardiaDB:GL50581_4481; -.
DR VEuPathDB; GiardiaDB:GL50803_0013220; -.
DR VEuPathDB; GiardiaDB:QR46_0919; -.
DR Proteomes; UP000018040; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR CDD; cd00268; DEADc; 1.
DR CDD; cd18787; SF2_C_DEAD; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR24031:SF91; ATP-DEPENDENT RNA HELICASE DDX24; 1.
DR PANTHER; PTHR24031; RNA HELICASE; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU365068};
KW Helicase {ECO:0000256|RuleBase:RU365068, ECO:0000313|EMBL:ESU44662.1};
KW Hydrolase {ECO:0000256|RuleBase:RU365068};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU365068};
KW RNA-binding {ECO:0000256|RuleBase:RU365068}.
FT DOMAIN 159..352
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 452..603
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT REGION 349..375
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 635..655
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 688..707
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 723..791
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 791 AA; 87991 MW; 4B0DC71CF988CAE1 CRC64;
MAWLFCTHFT FSYNFAQISS AINSMKPAET SILHRARWRP DPSLKLKNGD IYSGLVSLET
ISHKELFQLS LADEAKGTSR DTSSAEGSEG SDAFQSYPEN ELNVMADHNY KQDFVDEICS
LAGVEGIDEW VRLGLHPVLC KYLVRAGYTK PRAIQVQTIR AALHDKSLVI AAETGSGKTL
AYLLPALHKA FTLVTNALKR PEQAQRRALQ TLIIAPTREL ATQINTVMTT IFGDIQKCFR
IMYTIVGGIS PQKQERFIRE RPLILVGTPG RLYDISKSNA FFADTSLIDR LVIDESDKML
QKGRFHELID LCSALTSAVT DGCTAKVTIS SATMSLPSML RERNIKFSEP DEETAQPSNQ
GRTWKEKKRG RRKDNKFDKA IRSAKKDLVL EIADNAAVTE SQRQIYMSWL KVLSNLGFSG
SKLVIVDISP DCIVNKAVFE LWNHVFDDSE KNVLMAYLVI RYRYPTIIFV KTIDQAKNFT
NIFQLLGIPA WCVHASMPQR QRYKNVDRLR DRSDTVLITT DVCARGLDIP HVSLVIQHSA
PDSTSSHIHR VGRAARVESG ADQKHVGMAI SLISPADNNR FVQIASVCGY TQKTMPPAID
IQGDVLKSIK HIWAAASEAA DALAENQGFM RRQSYRRRDE KNFNSSSDES DDDFRTFINN
PEAQAISHEL SEHIQISKAR LEALLSRPIR SSSRPGKPPL QLVGAASKAS HDITDLLTEH
PRELKLGGHG RGGSDVPKAS PDASLNKSRK DTTPLKISHL DAPKKIGNVS GNIASTPKSS
RGSKSKRKIK L
//
