ID V7AV58_PHAVU Unreviewed; 586 AA.
AC V7AV58;
DT 19-FEB-2014, integrated into UniProtKB/TrEMBL.
DT 19-FEB-2014, sequence version 1.
DT 24-JAN-2024, entry version 33.
DE RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
GN ORFNames=PHAVU_009G135900g {ECO:0000313|EMBL:ESW09542.1};
OS Phaseolus vulgaris (Kidney bean) (French bean).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Phaseolus.
OX NCBI_TaxID=3885 {ECO:0000313|EMBL:ESW09542.1, ECO:0000313|Proteomes:UP000000226};
RN [1] {ECO:0000313|Proteomes:UP000000226}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. G19833 {ECO:0000313|Proteomes:UP000000226};
RX PubMed=24908249; DOI=10.1038/ng.3008;
RA Schmutz J., McClean P.E., Mamidi S., Wu G.A., Cannon S.B., Grimwood J.,
RA Jenkins J., Shu S., Song Q., Chavarro C., Torres-Torres M., Geffroy V.,
RA Moghaddam S.M., Gao D., Abernathy B., Barry K., Blair M., Brick M.A.,
RA Chovatia M., Gepts P., Goodstein D.M., Gonzales M., Hellsten U.,
RA Hyten D.L., Jia G., Kelly J.D., Kudrna D., Lee R., Richard M.M.,
RA Miklas P.N., Osorno J.M., Rodrigues J., Thareau V., Urrea C.A., Wang M.,
RA Yu Y., Zhang M., Wing R.A., Cregan P.B., Rokhsar D.S., Jackson S.A.;
RT "A reference genome for common bean and genome-wide analysis of dual
RT domestications.";
RL Nat. Genet. 46:707-713(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000966,
CC ECO:0000256|RuleBase:RU361166};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000256|ARBA:ARBA00007072, ECO:0000256|PROSITE-ProRule:PRU10059,
CC ECO:0000256|RuleBase:RU361166}.
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DR EMBL; CM002296; ESW09542.1; -; Genomic_DNA.
DR RefSeq; XP_007137548.1; XM_007137486.1.
DR AlphaFoldDB; V7AV58; -.
DR STRING; 3885.V7AV58; -.
DR EnsemblPlants; ESW09542; ESW09542; PHAVU_009G135900g.
DR GeneID; 18620069; -.
DR Gramene; ESW09542; ESW09542; PHAVU_009G135900g.
DR KEGG; pvu:PHAVU_009G135900g; -.
DR eggNOG; ENOG502QPI6; Eukaryota.
DR OMA; VWGGTWL; -.
DR OrthoDB; 1347382at2759; -.
DR Proteomes; UP000000226; Chromosome 9.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR PANTHER; PTHR22298:SF109; ENDOGLUCANASE; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR PROSITE; PS00592; GH9_2; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Cellulose degradation {ECO:0000256|ARBA:ARBA00023001,
KW ECO:0000256|RuleBase:RU361166};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU10059}; Membrane {ECO:0000256|SAM:Phobius};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Reference proteome {ECO:0000313|Proteomes:UP000000226};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 77..99
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 115..565
FT /note="Glycoside hydrolase family 9"
FT /evidence="ECO:0000259|Pfam:PF00759"
FT ACT_SITE 495
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10059"
SQ SEQUENCE 586 AA; 65416 MW; 43A8A29C3DB237DC CRC64;
MPPAVSSAFE SERAPVIYVH TVSEAGRLLP SASQWNSIAL DFKLAPHSST AYESIPSQYP
KSVDYDLVIT DRKHFHCFIF VLVTIILAIL AAVLLVYFLP QKHKHQGSSI NLKVAINQAL
TFYDAQKSGH YPSNSPVKFR GDSGLQDGDS ANTDLTGGFY DSGNNIKFTF TTAYTMTLLS
WTVMEYQSKY ADIGELDHVR DIIRWGSDYL LKVGSTIRIN NEHSDLSCWQ RPEDMKYERS
VSICDGSATD LAGEIVAALS AASMVFKDDR DYSKRLVEAA ERLFGAIPAK QGTYTMVDGC
GKQARMLYNS TSYQDELAWG ATWLFLATED TNYLATATET FFSAKGNESS VDKGVVYWNN
KLNAVEVLLT GIRYFRDPGF PYEDALKLSS NLTEAHMCSY LFSKYFSRTP GGLIILKPDN
EPLLQFAATA SFLSKLYSDY LDHLKMSGAS CETDSFSVKM LRDFATSQVN YILGQNPMKM
SYLVGYGDRY PVQVHHRSAS IPWNNQPYNC DDGKRWLNSK DPNPQVLLGA MVGGPDTNDN
FMDQRTNQRF TEPNIASNAG LVAALIALQD PPYNSRDVKS TLWGWT
//
