ID V7CNQ5_PHAVU Unreviewed; 3750 AA.
AC V7CNQ5;
DT 19-FEB-2014, integrated into UniProtKB/TrEMBL.
DT 19-FEB-2014, sequence version 1.
DT 27-MAR-2024, entry version 49.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN ORFNames=PHAVU_002G189700g {ECO:0000313|EMBL:ESW30875.1};
OS Phaseolus vulgaris (Kidney bean) (French bean).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Phaseolus.
OX NCBI_TaxID=3885 {ECO:0000313|EMBL:ESW30875.1, ECO:0000313|Proteomes:UP000000226};
RN [1] {ECO:0000313|Proteomes:UP000000226}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. G19833 {ECO:0000313|Proteomes:UP000000226};
RX PubMed=24908249; DOI=10.1038/ng.3008;
RA Schmutz J., McClean P.E., Mamidi S., Wu G.A., Cannon S.B., Grimwood J.,
RA Jenkins J., Shu S., Song Q., Chavarro C., Torres-Torres M., Geffroy V.,
RA Moghaddam S.M., Gao D., Abernathy B., Barry K., Blair M., Brick M.A.,
RA Chovatia M., Gepts P., Goodstein D.M., Gonzales M., Hellsten U.,
RA Hyten D.L., Jia G., Kelly J.D., Kudrna D., Lee R., Richard M.M.,
RA Miklas P.N., Osorno J.M., Rodrigues J., Thareau V., Urrea C.A., Wang M.,
RA Yu Y., Zhang M., Wing R.A., Cregan P.B., Rokhsar D.S., Jackson S.A.;
RT "A reference genome for common bean and genome-wide analysis of dual
RT domestications.";
RL Nat. Genet. 46:707-713(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SIMILARITY: Belongs to the UPL family. TOM1/PTR1 subfamily.
CC {ECO:0000256|ARBA:ARBA00034494}.
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DR EMBL; CM002289; ESW30875.1; -; Genomic_DNA.
DR RefSeq; XP_007158881.1; XM_007158819.1.
DR STRING; 3885.V7CNQ5; -.
DR EnsemblPlants; ESW30875; ESW30875; PHAVU_002G189700g.
DR GeneID; 18638423; -.
DR Gramene; ESW30875; ESW30875; PHAVU_002G189700g.
DR KEGG; pvu:PHAVU_002G189700g; -.
DR eggNOG; KOG0939; Eukaryota.
DR OMA; ADEMKYG; -.
DR OrthoDB; 1214833at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000000226; Chromosome 2.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd00078; HECTc; 1.
DR CDD; cd14327; UBA_atUPL1_2_like; 1.
DR Gene3D; 6.10.250.1630; -; 1.
DR Gene3D; 1.10.8.10; DNA helicase RuvA subunit, C-terminal domain; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR Gene3D; 1.25.10.10; Leucine-rich Repeat Variant; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR010309; E3_Ub_ligase_DUF908.
DR InterPro; IPR010314; E3_Ub_ligase_DUF913.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR025527; HUWE1/Rev1_UBM.
DR InterPro; IPR015940; UBA.
DR InterPro; IPR009060; UBA-like_sf.
DR InterPro; IPR003903; UIM_dom.
DR PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR PANTHER; PTHR11254:SF446; HECT-TYPE E3 UBIQUITIN TRANSFERASE; 1.
DR Pfam; PF06012; DUF908; 2.
DR Pfam; PF06025; DUF913; 1.
DR Pfam; PF00632; HECT; 1.
DR Pfam; PF00627; UBA; 1.
DR Pfam; PF14377; UBM; 3.
DR SMART; SM00119; HECTc; 1.
DR SMART; SM00165; UBA; 1.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR SUPFAM; SSF46934; UBA-like; 1.
DR PROSITE; PS50237; HECT; 1.
DR PROSITE; PS50030; UBA; 1.
DR PROSITE; PS50330; UIM; 1.
PE 3: Inferred from homology;
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000000226};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 1298..1339
FT /note="UBA"
FT /evidence="ECO:0000259|PROSITE:PS50030"
FT DOMAIN 3409..3750
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 910..940
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1521..1559
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2036..2057
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2153..2221
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2385..2405
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2425..2457
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2629..2653
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2956..2978
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3028..3069
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1521..1551
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2037..2051
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2157..2189
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2204..2221
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2425..2441
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2629..2652
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2959..2973
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3028..3057
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 3717
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 3750 AA; 411775 MW; 19F7F8B1176FD624 CRC64;
MKLKRKRALE VPPKIRCFID RVTSVPLEKI EEPLKGFVWE FDKGDFHHWV DLFNHFDSYF
EKYIKPRKDL LIDDDFLDLD PPFPRIAILQ ILRVIRTILD NCTNKHFYSS YEQHLSALLA
STDPDVVEAS LDTLATFLKK TVGKYSIRDT SLNSKLYALA QGWGGKEEGL GLIASCVPDG
CDRIACELGC TLHFEFYALN ESERDIKVAE PLVQGLQIIH LCDIDKRVET DLELLHKLVT
EYKVPASLRF SLLSRLRYAR AFGSLASRQQ YTCIRLYAFI VLIQACADAD DLVSFFNAEP
GFINELVSLL SYEDAVLERI RILCLHALAA LCQDRSRQQS VQTAVTSGGH RGILSSLMQK
AIDSVISDTS KWSVYFAEAL LSLVSVLVST SSGCSAMREA GFIPTLLPLL KDTNPQHLHL
VEKSVRILEA FMDYSNPAAA LFRDLGGLDD TISRLKIEVS HVENGGKQPD EKSEFSSRSV
NMVRSSSRLD DVQQPLYSEP LISYHRRLLM KALLRAISLG TYAPGNTARI YGSEENVLPH
CLCIIFRRAK DFGGGVFSLA ATVMSDLIQK DPTCFPVLDA AGLPSAFLDA IMDDVLNSSE
AITCIPQCLD ALCLNSNGLQ AVKDRNSLRC FVKVFTSKTY LRALAGDTPA SLSSGLDELM
RHAASLRGPG VEMLVEILES ISKIGSAVES SSLSSDPSSS TSVPMEMDGE EKNLILPNNE
SSKADDAGHI SEPSPDMSIM NVESFLPDCV NNIARLLETI LQNADTCRIF VEKKGIEAIL
QLVTLPLMPA SVSVGHSISV AFKNFSPQHY VSLARAVCSF LREHLRSTNE LLDLVGGTQL
ALVESAKQTK VLKYLSSLEA VLTLSVFLLK GTSTVVSELS TSDADVLKDL GKTYKEIIWQ
ISLCNDSKAE EKKNADQEPE VSQVPPSTAV ERESDDDSNI QTVRYTNPVF GRNGSHSLWS
GEREFLSVVR AGESLHRRSR HGISRIRGGR TGRHLEALNI DSEAPPSGLE APSSQDMKKK
SPDVLVSEIL NKLASTLRSF FTALVKGFTS PNRRRADSGS LSSASKTLGA VLATNFLEAL
SFSGHSTYAS GLELSLSVKC RYLGKVVDDM AALTFDSRRR SCYTAMVNNF YVHGTFKELL
TTFEATSQLL WTLPCSLPSP DNDVGKKGEG GKLSHNTWLL DTLQSYCRLL EYFVNSSHLL
SPTSASQAEL LVQPVAVGLS IGLFPVPRDP EVFVRMLQSQ VLDVILPVWN HPMFSSCSPG
FIASIISLVT HVYSGVGDVK RSRSNIVGST NQRFMPPPPD ETTIATIVEM GFSRARAEEA
LRRVETNSVE MAMEWLFSHT DDPVQEDDEL ARALALSLGS SSESTKAETA EKTIDVLTEE
GHVKKPPVDD ILAASVKLFQ TSDSVSFQLT DLLVTLCSQS KGDDRPKVIS YLLQQLKLCP
LDFSQDNCAL SVLAHILALL LFEDVSTREI AAQNGIISSI IDILTNFKGR QELGKELPVP
KCISALLLTL DQMVQSRPKV ENVEGTQTGS LPDSSGEHGS LQISDTVVPK EKNSNGNEKE
PAVAFESILG KSTGFATVEE SHKLLDVACD LIKQHVPAVV MQAVLQLCAR LTKTHALALQ
FLENGGLAAL FNLPRICFFP GYDSVVSAIV RHLLEDPQTL QTAMELEIRQ TLSGNRHSGR
VSPRSFLTSL APVISRDPNV FMKAAAAVCQ LETSGGRTVV VLSKEKEKEK SKSSSIEAGL
SSNECVRIPE SKSHDGQGKC LKSHKKVPVN LTQVIDQLLE IVLKYPPMKG MEESERDSTF
MEIDEPTMKV KGKSKVDEAA SIEPESEKST GLVKVTFVLK LLSDILLMYG HAVGVILRRD
SEMCQFRGSN QPSGHSGIIH HVLHRLLPLS VDKSAGPDDW RGKLSEKASW FLVVLCGRSG
EGRKRVTNEL VKELMSFSNF ESNSMRNSLL PDKRLFTFVD LVYSILSKNS SSGSLPGSGY
SPDIAKSMID GGIIQCLTSI LQVVDLDHPD APKIVNLILK GLEGLTRAAN ASEQIFKSDG
TEKKRSTGLN DRSDDQITAP SATEAVAHDQ NVGSQEAIID TMDNAHDQGT SQGDNCVDNP
NQSVEQDMRV DEGGTLAQDP PMELGMDFMR EEMGEGGVLH NPDQIEMTFH VENRADDDMG
DEDDDMGDDG DEDEDDDDGE DEDEDIAEDG GGMMSLADTD VEDHDDVGFG DEYNDEMIDE
DDDDFHENRV IEVRWREALD GLDHLQILGQ PGFIDVAAEP FEGVNVDDLF RLQSFERRRQ
TGRSSFERSA TEVNGFQHPL LVRPPPSGDF VSMWSSSGNS TSRDSDTLSS GNLDVAHFYM
FDAPILPYDH VPSSLFGDRL GGAAPPPLTD YSVGMGSLHL PGRRVLGNGR WTDDGQPQGS
AQAASIAQAV EEQFLAQLNS VAPASSPVER QLQNSGEQEN KSDALASHDG PILTAGTDST
CQQIESPEQE NGNGEEINVD SVARDTGEDL PANEPMSVQP VSLNIMPNGI DCTVIEGNVT
PDENVEIFVN SSNAAAIQCE RAADVLTSIH DVPVESMECN GSSTADGQHT NLELGGSGFE
TPNSGDCHIP SIYASADVDM AGTGAEGNQS EQPTVSEDRR DELLSAQNTE VAPDASQADQ
VSANNEASGA NTIDPTFLEA LPDDLRAEVL ASQQAQSVQP PAYAPPSAED IDPEFLAALP
PDIQAEVLAQ QRAQRVAQQA EGQPVDMDNA SIIATFPADL REEVLLTSSE AVLSALPSPL
LAEAQILRDR AMSHYQARSL FGSSHRLNNR RNGLGFDRRP VMDRGVGVTI GRRSALTDSL
KVKEIEGEPL LDATALKALI RLLRLSQPLG KGLLQRLLLN LCAHTVTMAT LIYLLLDMIE
PEAEGSVSRS ATLNSQRLFG CHSNTVYGQS QLLDGLPPLV FRRILEILTY LATNHSAVAK
LLFHFDQSII SDSSRPVNVH TNEKGKEKVT EEGPTLNPSK AETGVVPLVL FLKLLSRPLF
LRSNAHLEQV MGLIQVIVDT AASKLESQSQ SEKEMADTQN LSASEAPSNT EKDAPLVESD
SNQQDKRADM RVCHSEGKKN VDMYIIFLQL PQSDLRNLCS LLGREGLSDK MYMLAGEVLK
KLAFIVPSHR KFFTVELSES AHALTGSAIS ELVTLQKTNM LGLSAGSMAG AAILRVLQAL
SSLTSLNTVG EMDMDNGVDQ HDDQATIWNL NTALEPLWQE LSNCISAAEM QLGQSSFSPN
MSNINVAENL QGSSTSPPLP PGTQRLLPFI EAFFVLCEKL QANESFMQQD HGNATAREVK
ESAGCSASTS VKGGGDSLRK LDGAITFTRF AEKHRRLSNA FIRQNPGLLE KSLSMMLKAP
RLIDFDNKRA YFRSRIRQQH DQHLSGPLRI SVRRAYILED SYNQLRMRPT QDLKGRLNVQ
FQGEEGIDAG GLTREWYQLL SRVIFDKGAL LFTTVGNNAT FQPNPNSVYQ TEHLSYFKFV
GRVVGKALFD GQLLDVYFTR SFYKHILGVK VTYHDIEAVD PDYYKNLKWM LENDVSDVPD
LTFSMDADEE KHILYEKNEV TDYELKPGGR NIRVTEETKH EYVDLVAEHL LTNAIRPQIN
SFLEGFNELV PRELISIFND KELELLISGL PEIDLDDLKA NTEYTGYTVA SNVVQWFWEV
VKTFNKEDMA RLLQFVTGTS KVPLEGFKAL QGISGPQRFQ IHKAYGAPDR LPSAHTCFNQ
LDLPEYTSKE QLQERLLLAI HEASEGFGFG
//
