ID V7IPB3_SALET Unreviewed; 523 AA.
AC V7IPB3;
DT 19-FEB-2014, integrated into UniProtKB/TrEMBL.
DT 19-FEB-2014, sequence version 1.
DT 24-JAN-2024, entry version 28.
DE RecName: Full=Galactarate dehydratase (L-threo-forming) {ECO:0000256|HAMAP-Rule:MF_02031};
DE Short=GalcD {ECO:0000256|HAMAP-Rule:MF_02031};
DE EC=4.2.1.42 {ECO:0000256|HAMAP-Rule:MF_02031};
GN Name=garD {ECO:0000256|HAMAP-Rule:MF_02031};
GN ORFNames=A628_02818 {ECO:0000313|EMBL:ETA87139.1};
OS Salmonella enterica subsp. enterica serovar Cubana str. 76814.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=1192560 {ECO:0000313|EMBL:ETA87139.1, ECO:0000313|Proteomes:UP000018534};
RN [1] {ECO:0000313|EMBL:ETA87139.1, ECO:0000313|Proteomes:UP000018534}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=76814 {ECO:0000313|EMBL:ETA87139.1};
RX PubMed=24459266;
RA Benahmed F.H., Gopinath G.R., Wang H., Jean-Gilles Beaubrun J., Grim C.,
RA Cheng C.M., McClelland M., Ayers S., Abbott J., Desai P., Frye J.G.,
RA Weinstock G., Hammack T.S., Hanes D.E., Rasmussen M.A., Davidson M.K.;
RT "Whole-Genome Sequencing of Salmonella enterica subsp. enterica Serovar
RT Cubana Strains Isolated from Agricultural Sources.";
RL Genome Announc. 2:e01184-13(2014).
CC -!- FUNCTION: Catalyzes the dehydration of galactarate to form 5-dehydro-4-
CC deoxy-D-glucarate (5-KDG). {ECO:0000256|HAMAP-Rule:MF_02031}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=galactarate = 5-dehydro-4-deoxy-D-glucarate + H2O;
CC Xref=Rhea:RHEA:16005, ChEBI:CHEBI:15377, ChEBI:CHEBI:16537,
CC ChEBI:CHEBI:42819; EC=4.2.1.42; Evidence={ECO:0000256|HAMAP-
CC Rule:MF_02031};
CC -!- COFACTOR:
CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_02031};
CC -!- PATHWAY: Carbohydrate acid metabolism; galactarate degradation; D-
CC glycerate from galactarate: step 1/3. {ECO:0000256|HAMAP-
CC Rule:MF_02031}.
CC -!- SIMILARITY: Belongs to the UxaA family. {ECO:0000256|ARBA:ARBA00010986,
CC ECO:0000256|HAMAP-Rule:MF_02031}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ETA87139.1}.
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DR EMBL; AZGR01000059; ETA87139.1; -; Genomic_DNA.
DR RefSeq; WP_001273722.1; NZ_KI635685.1.
DR AlphaFoldDB; V7IPB3; -.
DR PATRIC; fig|1192560.4.peg.2636; -.
DR HOGENOM; CLU_029189_0_0_6; -.
DR UniPathway; UPA00565; UER00629.
DR Proteomes; UP000018534; Unassembled WGS sequence.
DR GO; GO:0008867; F:galactarate dehydratase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0046392; P:galactarate catabolic process; IEA:UniProtKB-UniRule.
DR CDD; cd11613; SAF_AH_GD; 1.
DR Gene3D; 2.30.130.110; -; 1.
DR HAMAP; MF_02031; Galactar_dehydrat; 1.
DR InterPro; IPR017654; GarD-like.
DR InterPro; IPR032893; GarD_Enterobacteriaceae.
DR InterPro; IPR048332; GD_AH_C.
DR InterPro; IPR007392; GD_AH_second.
DR InterPro; IPR013974; SAF.
DR InterPro; IPR044144; UxaA/GarD_SAF.
DR NCBIfam; TIGR03248; galactar-dH20; 1.
DR PANTHER; PTHR30536; ALTRONATE/GALACTARATE DEHYDRATASE; 1.
DR PANTHER; PTHR30536:SF1; GALACTARATE DEHYDRATASE (L-THREO-FORMING); 1.
DR Pfam; PF20629; GD_AH_C; 1.
DR Pfam; PF04295; GD_AH_second; 1.
DR Pfam; PF08666; SAF; 1.
DR SMART; SM00858; SAF; 1.
PE 3: Inferred from homology;
KW Iron {ECO:0000256|HAMAP-Rule:MF_02031};
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_02031};
KW Metal-binding {ECO:0000256|HAMAP-Rule:MF_02031}.
FT DOMAIN 22..92
FT /note="SAF"
FT /evidence="ECO:0000259|SMART:SM00858"
SQ SEQUENCE 523 AA; 56367 MW; F38EF5EAFCDF5AE4 CRC64;
MANIEIRQES PSAFYIKVHE TDNVAIIVND HGLKAGTRFP DGLELTEHIP QGHKVALTDI
PAHGEIIRYG EVIGYAVRDI PRGSWIDESL VELPKAPPLN TLPLATKVPE PLPPLEGYTF
EGYRNADGSV GTKNLLGITT SVHCVAGVVD YVVKVIERDL LPKYPNVDGV VGLNHLYGCG
VAINAPAAVV PIRTIHNIAL NPNFGGEVMV IGLGCEKLQP ERLLEGTEDV PAIAVESASI
VRLQDEQHVG FKSMVDDILR VAERHLTKLN QRQRETCPAS ELVVGMQCGG SDAFSGVTAN
PAVGYASDLL VRCGATVMFS EVTEVRDAIH LLTPRAINEA VGKRLLDEMA WYDNYLDMGK
TDRSANPSPG NKKGGLANVV EKALGSIAKS GKSAIVEVLS PGQRPTKRGL IYAATPASDF
VCGTQQVASG ITVQVFTTGR GTPYGLMAVP VIKMATRTEL ANRWYDLMDI NAGTIATGEE
TIEDVGWKLF HFILDVASGR KKTFSDQWGL HNQLAIFNPA PVT
//
