ID VP33A_RAT Reviewed; 597 AA.
AC Q63615;
DT 19-SEP-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 27-MAR-2024, entry version 119.
DE RecName: Full=Vacuolar protein sorting-associated protein 33A;
DE Short=r-vps33a;
GN Name=Vps33a;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain;
RX PubMed=8996080; DOI=10.1016/s0378-1119(96)00367-8;
RA Pevsner J., Hsu S.-C., Hyde P.S., Scheller R.H.;
RT "Mammalian homologues of yeast vacuolar protein sorting (vps) genes
RT implicated in Golgi-to-lysosome trafficking.";
RL Gene 183:7-14(1996).
RN [2]
RP CHARACTERIZATION, INTERACTION WITH VPS11 AND VPS18, SUBUNIT, AND
RP SUBCELLULAR LOCATION.
RX PubMed=11382755; DOI=10.1074/jbc.m101778200;
RA Kim B.Y., Kraemer H., Yamamoto A., Kominami E., Kohsaka S., Akazawa C.;
RT "Molecular characterization of mammalian homologues of class C Vps proteins
RT that interact with syntaxin-7.";
RL J. Biol. Chem. 276:29393-29402(2001).
CC -!- FUNCTION: Plays a role in vesicle-mediated protein trafficking to
CC lysosomal compartments including the endocytic membrane transport and
CC autophagic pathways. Believed to act as a core component of the
CC putative HOPS and CORVET endosomal tethering complexes which are
CC proposed to be involved in the Rab5-to-Rab7 endosome conversion
CC probably implicating MON1A/B, and via binding SNAREs and SNARE
CC complexes to mediate tethering and docking events during SNARE-mediated
CC membrane fusion. The HOPS complex is proposed to be recruited to Rab7
CC on the late endosomal membrane and to regulate late endocytic,
CC phagocytic and autophagic traffic towards lysosomes. The CORVET complex
CC is proposed to function as a Rab5 effector to mediate early endosome
CC fusion probably in specific endosome subpopulations. Required for
CC fusion of endosomes and autophagosomes with lysosomes; the function is
CC dependent on its association with VPS16 but not VIPAS39. The function
CC in autophagosome-lysosome fusion implicates STX17 but not UVRAG.
CC {ECO:0000250|UniProtKB:Q96AX1}.
CC -!- SUBUNIT: Core component of at least two putative endosomal tethering
CC complexes, the homotypic fusion and vacuole protein sorting (HOPS)
CC complex and the class C core vacuole/endosome tethering (CORVET)
CC complex. Their common core is composed of the class C Vps proteins
CC VPS11, VPS16, VPS18 and VPS33A, which in HOPS further associates with
CC VPS39 and VPS41 and in CORVET with VPS8 and TGFBRAP1 (PubMed:11382755).
CC Interacts with RAB5C, UVRAG, STX17, MON1A and MON1B. Associates with
CC adaptor protein complex 3 (AP-3) and clathrin (By similarity).
CC Interacts with PLEKHM1 (By similarity). {ECO:0000250|UniProtKB:Q96AX1,
CC ECO:0000250|UniProtKB:Q9D2N9, ECO:0000305|PubMed:11382755}.
CC -!- INTERACTION:
CC Q63615; Q9H270: VPS11; Xeno; NbExp=4; IntAct=EBI-918669, EBI-373380;
CC Q63615; Q9P253: VPS18; Xeno; NbExp=2; IntAct=EBI-918669, EBI-1053363;
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle
CC {ECO:0000269|PubMed:11382755}. Late endosome membrane {ECO:0000305};
CC Peripheral membrane protein {ECO:0000305}; Cytoplasmic side
CC {ECO:0000305}. Lysosome membrane {ECO:0000305}; Peripheral membrane
CC protein {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Early endosome
CC {ECO:0000305}. Cytoplasmic vesicle, autophagosome
CC {ECO:0000250|UniProtKB:Q96AX1}. Cytoplasmic vesicle, clathrin-coated
CC vesicle {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Ubiquitous.
CC -!- SIMILARITY: Belongs to the STXBP/unc-18/SEC1 family. {ECO:0000305}.
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DR EMBL; U35244; AAC52985.1; -; mRNA.
DR PIR; JC5720; JC5720.
DR AlphaFoldDB; Q63615; -.
DR SMR; Q63615; -.
DR CORUM; Q63615; -.
DR IntAct; Q63615; 5.
DR STRING; 10116.ENSRNOP00000073489; -.
DR PhosphoSitePlus; Q63615; -.
DR jPOST; Q63615; -.
DR PaxDb; 10116-ENSRNOP00000059816; -.
DR UCSC; RGD:620643; rat.
DR AGR; RGD:620643; -.
DR RGD; 620643; Vps33a.
DR eggNOG; KOG1302; Eukaryota.
DR InParanoid; Q63615; -.
DR PhylomeDB; Q63615; -.
DR PRO; PR:Q63615; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0030123; C:AP-3 adaptor complex; ISO:RGD.
DR GO; GO:0005776; C:autophagosome; ISS:UniProtKB.
DR GO; GO:0071439; C:clathrin complex; ISO:RGD.
DR GO; GO:0030136; C:clathrin-coated vesicle; IEA:UniProtKB-SubCell.
DR GO; GO:0033263; C:CORVET complex; ISO:RGD.
DR GO; GO:0005769; C:early endosome; ISO:RGD.
DR GO; GO:0030897; C:HOPS complex; ISO:RGD.
DR GO; GO:0005770; C:late endosome; ISO:RGD.
DR GO; GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005764; C:lysosome; ISS:UniProtKB.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; ISO:RGD.
DR GO; GO:0044877; F:protein-containing complex binding; IPI:RGD.
DR GO; GO:0097352; P:autophagosome maturation; ISO:RGD.
DR GO; GO:0008333; P:endosome to lysosome transport; ISO:RGD.
DR GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR GO; GO:0032418; P:lysosome localization; ISO:RGD.
DR GO; GO:0032400; P:melanosome localization; ISO:RGD.
DR GO; GO:0043473; P:pigmentation; ISO:RGD.
DR GO; GO:0030220; P:platelet formation; ISO:RGD.
DR GO; GO:0048070; P:regulation of developmental pigmentation; ISO:RGD.
DR GO; GO:0035751; P:regulation of lysosomal lumen pH; ISS:UniProtKB.
DR GO; GO:0016192; P:vesicle-mediated transport; ISO:RGD.
DR Gene3D; 1.25.40.850; -; 1.
DR Gene3D; 3.40.50.1910; -; 3.
DR Gene3D; 3.40.50.2060; -; 1.
DR InterPro; IPR043154; Sec-1-like_dom1.
DR InterPro; IPR001619; Sec1-like.
DR InterPro; IPR027482; Sec1-like_dom2.
DR InterPro; IPR036045; Sec1-like_sf.
DR InterPro; IPR043155; VPS33_dom3b.
DR PANTHER; PTHR11679:SF85; VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 33A; 1.
DR PANTHER; PTHR11679; VESICLE PROTEIN SORTING-ASSOCIATED; 1.
DR Pfam; PF00995; Sec1; 1.
DR SUPFAM; SSF56815; Sec1/munc18-like (SM) proteins; 1.
PE 1: Evidence at protein level;
KW Cytoplasmic vesicle; Endosome; Lysosome; Membrane; Protein transport;
KW Reference proteome; Transport.
FT CHAIN 1..597
FT /note="Vacuolar protein sorting-associated protein 33A"
FT /id="PRO_0000206304"
SQ SEQUENCE 597 AA; 67514 MW; 455A3362D56E5188 CRC64;
MAAHLSYGRV NLNVLREAVR RELREFLDKC AGSKAIVWDE YLTGPFGLIA QYSLLKEHEV
EKMFTLKGSR LPAADVKNII FLVRPRLELM DMIAENVLSE DRRGPTRDFH ILFVPRRSLL
CEQRLKDLGV LGSFIYREEY SLDLIPFDGD LLSMESESAF KECYLEGDQT SLYHAAKGLM
TLQALYGTIP QIFGKGECAR QVANMMVRMK REFTGSQNSV FPVFDNLLLL DRNVDLLTPL
ASQLTYEGLI DEIYGIQNSY VKLPPEKFAP KKQGGGGGKD LPTEAKKLQL NSAEELYAEI
RDKNFNAVGS VLSKKAKIIS AAFEERHNAK TVGEIKQFVS QLPHMQAARG SLANHTSIAE
LIKDVTTSED FFDKLTVEQE FMSGIDTDKV NNYIEDCIAQ KHPLIKVLRL VCLQSMCNSG
LKQKVLDYYK REILQTYGYE HILTLNNLEK AGLLKAQTGG RNNYPTIRKT LRLWMDDVNE
QNPTDISYVY SGYAPLSVRL AQLLSRPGWR SIEEVLRILP GPHFEERQPL PTGVQKKRQP
GENRVTLVFF LGGVTFAEIA ALRFLSQLED GGTEYVIATT KLINGSSWLE ALMEKPF
//
