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Database: UniProt
Entry: VPP1_RAT
LinkDB: VPP1_RAT
Original site: VPP1_RAT 
ID   VPP1_RAT                Reviewed;         838 AA.
AC   P25286;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 1.
DT   22-NOV-2017, entry version 123.
DE   RecName: Full=V-type proton ATPase 116 kDa subunit a isoform 1;
DE            Short=V-ATPase 116 kDa isoform a1;
DE   AltName: Full=Clathrin-coated vesicle/synaptic vesicle proton pump 116 kDa subunit;
DE   AltName: Full=Vacuolar adenosine triphosphatase subunit Ac116;
DE   AltName: Full=Vacuolar proton pump subunit 1;
DE   AltName: Full=Vacuolar proton translocating ATPase 116 kDa subunit a isoform 1;
GN   Name=Atp6v0a1; Synonyms=Atp6n1, Vpp1;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
OC   Muroidea; Muridae; Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS I AND II).
RC   TISSUE=Brain;
RX   PubMed=1704894;
RA   Perin M.S., Fried V.A., Stone D.K., Xie X.-S., Suedhof T.C.;
RT   "Structure of the 116-kDa polypeptide of the clathrin-coated
RT   vesicle/synaptic vesicle proton pump.";
RL   J. Biol. Chem. 266:3877-3881(1991).
CC   -!- FUNCTION: Required for assembly and activity of the vacuolar
CC       ATPase. Potential role in differential targeting and regulation of
CC       the enzyme for a specific organelle (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: The V-ATPase is a heteromultimeric enzyme composed of at
CC       least thirteen different subunits. It has a membrane peripheral V1
CC       sector for ATP hydrolysis and an integral V0 for proton
CC       translocation. The V1 sector comprises subunits A-H, whereas V0
CC       includes subunits a, d, c, c', and c''. Interacts with SPAAR.
CC       {ECO:0000250|UniProtKB:Q93050}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane; Multi-pass
CC       membrane protein. Melanosome {ECO:0000250}. Note=Coated vesicle.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=I;
CC         IsoId=P25286-1; Sequence=Displayed;
CC       Name=II;
CC         IsoId=P25286-2; Sequence=VSP_000344;
CC   -!- SIMILARITY: Belongs to the V-ATPase 116 kDa subunit family.
CC       {ECO:0000305}.
DR   EMBL; M58758; AAA41962.1; -; mRNA.
DR   PIR; B38656; B38656.
DR   RefSeq; NP_113792.2; NM_031604.2.
DR   UniGene; Rn.6015; -.
DR   BioGrid; 248368; 3.
DR   IntAct; P25286; 2.
DR   STRING; 10116.ENSRNOP00000052113; -.
DR   iPTMnet; P25286; -.
DR   PhosphoSitePlus; P25286; -.
DR   PaxDb; P25286; -.
DR   PRIDE; P25286; -.
DR   GeneID; 29757; -.
DR   KEGG; rno:29757; -.
DR   UCSC; RGD:68405; rat. [P25286-1]
DR   CTD; 535; -.
DR   RGD; 68405; Atp6v0a1.
DR   eggNOG; KOG2189; Eukaryota.
DR   eggNOG; COG1269; LUCA.
DR   HOVERGEN; HBG014606; -.
DR   InParanoid; P25286; -.
DR   KO; K02154; -.
DR   PhylomeDB; P25286; -.
DR   PRO; PR:P25286; -.
DR   Proteomes; UP000002494; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR   GO; GO:0070062; C:extracellular exosome; ISO:RGD.
DR   GO; GO:0030285; C:integral component of synaptic vesicle membrane; IDA:SynGO.
DR   GO; GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; ISO:RGD.
DR   GO; GO:0008021; C:synaptic vesicle; IDA:SynGO.
DR   GO; GO:0043195; C:terminal bouton; IDA:ParkinsonsUK-UCL.
DR   GO; GO:0016471; C:vacuolar proton-transporting V-type ATPase complex; IBA:GO_Central.
DR   GO; GO:0000220; C:vacuolar proton-transporting V-type ATPase, V0 domain; IEA:InterPro.
DR   GO; GO:0051117; F:ATPase binding; ISO:RGD.
DR   GO; GO:0046961; F:proton-transporting ATPase activity, rotational mechanism; IBA:GO_Central.
DR   GO; GO:0015991; P:ATP hydrolysis coupled proton transport; IEA:InterPro.
DR   GO; GO:0015986; P:ATP synthesis coupled proton transport; IBA:GO_Central.
DR   GO; GO:0016241; P:regulation of macroautophagy; ISO:RGD.
DR   GO; GO:1901998; P:toxin transport; ISO:RGD.
DR   GO; GO:0007035; P:vacuolar acidification; IBA:GO_Central.
DR   GO; GO:0070072; P:vacuolar proton-transporting V-type ATPase complex assembly; IBA:GO_Central.
DR   InterPro; IPR002490; V-ATPase_116kDa_su.
DR   InterPro; IPR026028; V-type_ATPase_116kDa_su_euka.
DR   PANTHER; PTHR11629; PTHR11629; 1.
DR   Pfam; PF01496; V_ATPase_I; 1.
DR   PIRSF; PIRSF001293; ATP6V0A1; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Complete proteome; Cytoplasmic vesicle;
KW   Hydrogen ion transport; Ion transport; Membrane; Phosphoprotein;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN         1    838       V-type proton ATPase 116 kDa subunit a
FT                                isoform 1.
FT                                /FTId=PRO_0000119214.
FT   TOPO_DOM      1    388       Cytoplasmic. {ECO:0000255}.
FT   TRANSMEM    389    407       Helical. {ECO:0000255}.
FT   TOPO_DOM    408    409       Vacuolar. {ECO:0000255}.
FT   TRANSMEM    410    426       Helical. {ECO:0000255}.
FT   TOPO_DOM    427    441       Cytoplasmic. {ECO:0000255}.
FT   TRANSMEM    442    471       Helical. {ECO:0000255}.
FT   TOPO_DOM    472    535       Vacuolar. {ECO:0000255}.
FT   TRANSMEM    536    555       Helical. {ECO:0000255}.
FT   TOPO_DOM    556    573       Cytoplasmic. {ECO:0000255}.
FT   TRANSMEM    574    594       Helical. {ECO:0000255}.
FT   TOPO_DOM    595    639       Vacuolar. {ECO:0000255}.
FT   TRANSMEM    640    659       Helical. {ECO:0000255}.
FT   TOPO_DOM    660    725       Cytoplasmic. {ECO:0000255}.
FT   TRANSMEM    726    750       Helical. {ECO:0000255}.
FT   TOPO_DOM    751    771       Vacuolar. {ECO:0000255}.
FT   TRANSMEM    772    810       Helical. {ECO:0000255}.
FT   TOPO_DOM    811    838       Cytoplasmic. {ECO:0000255}.
FT   MOD_RES     250    250       Phosphothreonine.
FT                                {ECO:0000250|UniProtKB:Q9Z1G4}.
FT   MOD_RES     360    360       Phosphothreonine.
FT                                {ECO:0000250|UniProtKB:Q93050}.
FT   MOD_RES     364    364       Phosphotyrosine.
FT                                {ECO:0000250|UniProtKB:Q9Z1G4}.
FT   VAR_SEQ     706    711       Missing (in isoform II).
FT                                {ECO:0000303|PubMed:1704894}.
FT                                /FTId=VSP_000344.
SQ   SEQUENCE   838 AA;  96328 MW;  D25FAD918638D7EE CRC64;
     MGELFRSEEM TLAQLFLQSE AAYCCVSELE ELGKVQFRDL NPDVNVFQRK FVNEVRRCEE
     MDRKLRFVEK EIRKANIPIM DTGENPEVPF PRDMIDLEAN FEKIENELKE INTNQEALKR
     NFLELTELKF ILRKTQQFFD EMADPDLLEE SSSLLEPNEM GRGAPLRLGF VAGVINRERI
     PTFERMLWRV CRGNVFLRQA EIENPLEDPV TGDYVHKSVF IIFFQGDQLK NRVKKICEGF
     RASLYPCPET PQERKEMASG VNTRIDDLQM VLNQTEDHRQ RVLQAAAKNI RVWFIKVRKM
     KAIYHTLNLC NIDVTQKCLI AEVWCPVTDL DSIQFALRRG TEHSGSTVPS ILNRMQTNQT
     PPTYNKTNKF THGFQNIVDA YGIGTYREIN PAPYTVITFP FLFAVMFGDF GHGILMTLFA
     VWMVLRESRI LSQKNENEMF SMVFSGRYII LLMGLFSIYT GLIYNDCFSK SLNIFGSSWS
     VRPMFTIGNW TEETLLGSSV LQLNPAIPGV FGGPYPFGID PIWNIATNKL TFLNSFKMKM
     SVILGIIHML FGVSLSLFNH IYFKKPLNIY FGFIPEIIFM SSLFGYLVIL IFYKWTAYDA
     HSSRNAPSLL IHFINMFLFS YPESGNAMLY SGQKGIQCFL IVVAMLCVPW MLLFKPLILR
     HQYLRKKHLG TLNFGGIRVG NGPTEEDAEI IQHDQLSTHS EDAEEPTEDE VFDFGDTMVH
     QAIHTIEYCL GCISNTASYL RLWALSLAHA QLSEVLWTMV IHIGLHVRSL AGGLGLFFIF
     AAFATLTVAI LLIMEGLSAF LHALRLHWVE FQNKFYTGTG FKFLPFSFEH IREGKFDE
//
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