ID W0RMS2_9BACT Unreviewed; 880 AA.
AC W0RMS2;
DT 19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT 19-MAR-2014, sequence version 1.
DT 27-MAR-2024, entry version 47.
DE RecName: Full=Aconitate hydratase {ECO:0000256|RuleBase:RU361275};
DE Short=Aconitase {ECO:0000256|RuleBase:RU361275};
DE EC=4.2.1.3 {ECO:0000256|RuleBase:RU361275};
GN ORFNames=J421_4230 {ECO:0000313|EMBL:AHG91767.1};
OS Gemmatirosa kalamazoonensis.
OC Bacteria; Gemmatimonadota; Gemmatimonadetes; Gemmatimonadales;
OC Gemmatimonadaceae; Gemmatirosa.
OX NCBI_TaxID=861299 {ECO:0000313|EMBL:AHG91767.1, ECO:0000313|Proteomes:UP000019151};
RN [1] {ECO:0000313|EMBL:AHG91767.1, ECO:0000313|Proteomes:UP000019151}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KBS708 {ECO:0000313|EMBL:AHG91767.1,
RC ECO:0000313|Proteomes:UP000019151};
RX PubMed=24699952;
RA Debruyn J.M., Radosevich M., Wommack K.E., Polson S.W., Hauser L.J.,
RA Fawaz M.N., Korlach J., Tsai Y.C.;
RT "Genome Sequence and Methylome of Soil Bacterium Gemmatirosa
RT kalamazoonensis KBS708T, a Member of the Rarely Cultivated Gemmatimonadetes
RT Phylum.";
RL Genome Announc. 2:e00226-14(2014).
CC -!- FUNCTION: Catalyzes the isomerization of citrate to isocitrate via cis-
CC aconitate. {ECO:0000256|RuleBase:RU361275}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=citrate = D-threo-isocitrate; Xref=Rhea:RHEA:10336,
CC ChEBI:CHEBI:15562, ChEBI:CHEBI:16947; EC=4.2.1.3;
CC Evidence={ECO:0000256|ARBA:ARBA00023501,
CC ECO:0000256|RuleBase:RU361275};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC from oxaloacetate: step 2/2. {ECO:0000256|ARBA:ARBA00004717}.
CC -!- SIMILARITY: Belongs to the aconitase/IPM isomerase family.
CC {ECO:0000256|ARBA:ARBA00007185, ECO:0000256|RuleBase:RU361275}.
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DR EMBL; CP007128; AHG91767.1; -; Genomic_DNA.
DR AlphaFoldDB; W0RMS2; -.
DR STRING; 861299.J421_4230; -.
DR KEGG; gba:J421_4230; -.
DR PATRIC; fig|861299.3.peg.4287; -.
DR eggNOG; COG1048; Bacteria.
DR HOGENOM; CLU_013476_2_1_0; -.
DR InParanoid; W0RMS2; -.
DR UniPathway; UPA00223; UER00718.
DR Proteomes; UP000019151; Chromosome.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003994; F:aconitate hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR CDD; cd01586; AcnA_IRP; 1.
DR CDD; cd01580; AcnA_IRP_Swivel; 1.
DR Gene3D; 6.10.190.10; -; 1.
DR Gene3D; 3.30.499.10; Aconitase, domain 3; 2.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR InterPro; IPR044137; AcnA_IRP_Swivel.
DR InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR006249; Aconitase/IRP2.
DR InterPro; IPR018136; Aconitase_4Fe-4S_BS.
DR InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR NCBIfam; TIGR01341; aconitase_1; 1.
DR PANTHER; PTHR11670; ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER; 1.
DR PANTHER; PTHR11670:SF54; CYTOPLASMIC ACONITATE HYDRATASE; 1.
DR Pfam; PF00330; Aconitase; 1.
DR Pfam; PF00694; Aconitase_C; 1.
DR PRINTS; PR00415; ACONITASE.
DR SUPFAM; SSF53732; Aconitase iron-sulfur domain; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
DR PROSITE; PS00450; ACONITASE_1; 1.
DR PROSITE; PS01244; ACONITASE_2; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|RuleBase:RU361275};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|RuleBase:RU361275};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014, ECO:0000256|RuleBase:RU361275};
KW Lyase {ECO:0000256|RuleBase:RU361275};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000019151}.
FT DOMAIN 32..548
FT /note="Aconitase/3-isopropylmalate dehydratase large
FT subunit alpha/beta/alpha"
FT /evidence="ECO:0000259|Pfam:PF00330"
FT DOMAIN 678..804
FT /note="Aconitase A/isopropylmalate dehydratase small
FT subunit swivel"
FT /evidence="ECO:0000259|Pfam:PF00694"
SQ SEQUENCE 880 AA; 94750 MW; 4A0CD7ED3559C370 CRC64;
MLLENLLRGE DGAFVKRADV EALARWNVAA PSEKEVAYRP ARVLLQDFTG VPCVVDLAAM
RDAITALGGD AQRINPLQPV DLVIDHSVQA DEYGTPQAFL ENVSFEFERN QERYRFLRWG
QSAFRNFSVV PPGTGICHQV NLEYLARVVF VGDGNVAYPD SLVGTDSHTT MVNGLGVFGW
GVGGIEAEAA MLGQPLSMLV PDVVGFKLTG KLPAGATATD LVLTVTEMLR KKKVVGKFVE
YYGTGLSGLP LADRATIANM APEYGATMGF FPVDEETLKY LRLTGRSAEQ IALVEAYTKA
QGLFRTDATP DPVYSDTLEL DLSTVEPSIA GPRRPQDRIP LRQSKELFAT HLGEVLAKAG
SAAGKAEIHA AKEVSAPTEL AESAVATVTA EQEADGVEVE YNGQTFRLRN GAVVIAAITS
CTNTSNPSVM LGAGLLARKA AAKGLKAKPW VKSTLSPGSR VVTDYYEKAD LMKDLEAVGF
YLAGYGCMTC IGNSGPLPDP IAAAVEKGKL VVSAVLSGNR NFEGRVNPHT RFNYLASPML
VVAYALAGRM DIDLEREPLG VGAQGPVFLR DVWPTPQEIQ DTILQAVKGE MFARQYANVF
EGDEQWRALD VPTGDTYAWD PNSTYVANPP YFEGMTMAPK GVAPIAGARC LAMLGDSITT
DHISPAGNIS AASPAGKWLI AHGVKTREFN SYGARRGHHE VMMRGTFANI RLRNELVPGT
EGGWTTTEPG GDAVSIYDAA MQYQQQGTPL VIVAGKEYGT GSSRDWAAKG TVLLGVRAVI
AESFERIHRS NLVGMGVLPL EFTNGESRTS HGLTGFETYE IVGLDDTLTP RKRLTVKATA
PDGSVKSFEV LARIDTPEEL SYFKHGGILP YVLRSLVKRA
//
