ID W4Q6Q7_9BACI Unreviewed; 941 AA.
AC W4Q6Q7;
DT 19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT 19-MAR-2014, sequence version 1.
DT 24-JAN-2024, entry version 34.
DE RecName: Full=cellulase {ECO:0000256|ARBA:ARBA00012601};
DE EC=3.2.1.4 {ECO:0000256|ARBA:ARBA00012601};
GN ORFNames=JCM9140_3161 {ECO:0000313|EMBL:GAE27049.1};
OS Halalkalibacter wakoensis JCM 9140.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Halalkalibacter.
OX NCBI_TaxID=1236970 {ECO:0000313|EMBL:GAE27049.1, ECO:0000313|Proteomes:UP000018890};
RN [1] {ECO:0000313|EMBL:GAE27049.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 9140 {ECO:0000313|EMBL:GAE27049.1};
RA Yuki M., Oshima K., Suda W., Oshida Y., Kitamura K., Iida T., Hattori M.,
RA Ohkuma M.;
RT "Draft Genome Sequences of Three Alkaliphilic Bacillus Strains, Bacillus
RT wakoensis JCM 9140T, Bacillus akibai JCM 9157T, and Bacillus
RT hemicellulosilyticus JCM 9152T.";
RL Genome Announc. 2:e01258-13(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000966};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC {ECO:0000256|ARBA:ARBA00005641}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAE27049.1}.
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DR EMBL; BAUT01000038; GAE27049.1; -; Genomic_DNA.
DR RefSeq; WP_052002277.1; NZ_BAZN01000038.1.
DR AlphaFoldDB; W4Q6Q7; -.
DR SMR; W4Q6Q7; -.
DR STRING; 1236970.JCM9140_3161; -.
DR OrthoDB; 154460at2; -.
DR Proteomes; UP000018890; Unassembled WGS sequence.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 2.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR005086; CBM_fam_17/28.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR001547; Glyco_hydro_5.
DR InterPro; IPR018087; Glyco_hydro_5_CS.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR001119; SLH_dom.
DR PANTHER; PTHR34142:SF1; CELLULASE DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR34142; ENDO-BETA-1,4-GLUCANASE A; 1.
DR Pfam; PF03424; CBM_17_28; 1.
DR Pfam; PF00150; Cellulase; 1.
DR Pfam; PF00395; SLH; 3.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 2.
DR PROSITE; PS00659; GLYCOSYL_HYDROL_F5; 1.
DR PROSITE; PS51272; SLH; 3.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023326};
KW Cellulose degradation {ECO:0000256|ARBA:ARBA00023001};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326};
KW Reference proteome {ECO:0000313|Proteomes:UP000018890};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..29
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 30..941
FT /note="cellulase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5004848311"
FT DOMAIN 37..94
FT /note="SLH"
FT /evidence="ECO:0000259|PROSITE:PS51272"
FT DOMAIN 95..158
FT /note="SLH"
FT /evidence="ECO:0000259|PROSITE:PS51272"
FT DOMAIN 161..224
FT /note="SLH"
FT /evidence="ECO:0000259|PROSITE:PS51272"
SQ SEQUENCE 941 AA; 104628 MW; BEA2AC3B169BFADA CRC64;
MKIKQIKQSL SLLLIITLIM SLFVPMASAN TNESKSNAFP FSDVKKTSWS FPYIKDLYEQ
EVITGTSATT FSPTDSVTRA QFTVMLTRGL GLEASSKDYP FKDRKNWAYK EIQAAYEAGI
VTGKTNGEFA PNENITREQM AAMAVRAYEY LENELSLPEE QREYNDSSSI STFAQDAVQK
AYVLELMEGN TDGYFQPKRN STREQSAKVI STLLWKVASH DYLYHTEAVK SPSEAGALQL
VELNGQLTLA GEDGTPVQLR GMSTHGLQWF GEIVNENAFV ALSNDWGSNM IRLAMYIGEN
GYATNPEVKD LVYEGIELAF EHDMYVIVDW HVHAPGDPRA DVYSGAYDFF EEIADHYKDH
PKNHYIIWEL ANEPSPNNNG GPGLTNDEKG WEAVKEYAEP IVEMLREKGD NMILVGNPNW
SQRPDLSADN PIDAENIMYS VHFYTGSHGA SHIGYPEGTP SSERSNVMAN VRYALDNGVA
VFATEWGTSQ ANGDGGPYFD EADVWLNFLN KHNISWANWS LTNKNEISGA FTPFELGRTD
ATDLDPGANQ VWAPEELSLS GEYVRARIKG IEYTPIDRTK FTKLVWDFND GTTQGFQVNG
DSPNKESITL SNNNDALQIE GLNVSNDISE GNYWDNVRLS ADGWSENVDI LGATELTIDV
IVEEPTTVSI AAIPQGPAAG WANPTRAIKV TEDDFESFGD GYKALVTITS EDSPSLETIA
TSPEDNTMSN IILFVGTEDA DVISLDNITV SGTEIEIEVI HDEKGTATLP STFEDGTRQG
WDWHTESGVK TALTIEEANG SNALSWEYAY PEVKPSDGWA TAPRLDFWKD ELVRGTSDYI
SFDFYIDAVR ASEGAISINA VFQPPANGYW QEVPTTFEID LTELDSATVT SDELYHYEVK
INIRDIEAIT DDTELRNLLL IFADEDSDFA GRVFVDNVRF E
//
