ID W4QBX8_9BACI Unreviewed; 516 AA.
AC W4QBX8;
DT 19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT 19-MAR-2014, sequence version 1.
DT 24-JAN-2024, entry version 24.
DE SubName: Full=Beta-xylosidase {ECO:0000313|EMBL:GAE29561.1};
GN ORFNames=JCM9152_925 {ECO:0000313|EMBL:GAE29561.1};
OS Halalkalibacter hemicellulosilyticusJCM 9152.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Halalkalibacter.
OX NCBI_TaxID=1236971 {ECO:0000313|EMBL:GAE29561.1, ECO:0000313|Proteomes:UP000018895};
RN [1] {ECO:0000313|EMBL:GAE29561.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 9152 {ECO:0000313|EMBL:GAE29561.1};
RA Yuki M., Oshima K., Suda W., Oshida Y., Kitamura K., Iida T., Hattori M.,
RA Ohkuma M.;
RT "Draft Genome Sequences of Three Alkaliphilic Bacillus Strains, Bacillus
RT wakoensis JCM 9140T, Bacillus akibai JCM 9157T, and Bacillus
RT hemicellulosilyticus JCM 9152T.";
RL Genome Announc. 2:e01258-13(2014).
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAE29561.1}.
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DR EMBL; BAUU01000005; GAE29561.1; -; Genomic_DNA.
DR RefSeq; WP_035341327.1; NZ_BAZO01000005.1.
DR AlphaFoldDB; W4QBX8; -.
DR STRING; 1236971.JCM9152_925; -.
DR OrthoDB; 9801455at2; -.
DR Proteomes; UP000018895; Unassembled WGS sequence.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd18617; GH43_XynB-like; 1.
DR Gene3D; 2.60.120.200; -; 1.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR041542; GH43_C2.
DR InterPro; IPR006710; Glyco_hydro_43.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR42812; BETA-XYLOSIDASE; 1.
DR PANTHER; PTHR42812:SF12; BETA-XYLOSIDASE-RELATED; 1.
DR Pfam; PF17851; GH43_C2; 1.
DR Pfam; PF04616; Glyco_hydro_43; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361187};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361187};
KW Reference proteome {ECO:0000313|Proteomes:UP000018895}.
FT DOMAIN 319..512
FT /note="Beta-xylosidase C-terminal Concanavalin A-like"
FT /evidence="ECO:0000259|Pfam:PF17851"
FT ACT_SITE 15
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT ACT_SITE 183
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT SITE 121
FT /note="Important for catalytic activity, responsible for
FT pKa modulation of the active site Glu and correct
FT orientation of both the proton donor and substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ SEQUENCE 516 AA; 58309 MW; 3469BF25481EAE0F CRC64;
MKTFNNPILP GFYPDPSICR VHDDYYLVTS SFAYYPGVPI FHSKDLVNWE QIGHVLDRPT
QLPLDGQKQS QGIFAPTIRY HDGVFYMITT NVGHGGNFFV TATDPAGPWS DPYYLDAPGI
DPSLFFDDDG KVYYTGTRPA PEGEKYSGNW EVWLQELDLE TKQLVGEPTG LWRGALRDVI
WPEGPHLYKV NGCYYLLISE AGTGHHHAVS VARSEHVSGP YVGNPGNPIL THRHLGKDYP
IVNVGHCDLV DTQNGEWWAV GLASRIYGGY YRNLGRETFL LPVTWEDGWP IMSPGTGKLE
MTYPLPDLPS VKSLVPSPCD HFEDEKLDFK WNFIRTPKEE FYSLTERPGH LSLKLRAQQM
SELEQPSFVG RRQQHIHFAA ATAMEFIPTN ENEEAGLVLL QSHEYHFRFV YTKQGNQQVM
RLIKCTEGIE EVLKELPIDA AKLYLKVQAR GQDYSFYFGE TTANYKALLE NVDGRILSTD
VAGGFVGAVL GMYASSNGIS SDEKAYFDWF EYVGIE
//
