ID W5KD30_ASTMX Unreviewed; 1501 AA.
AC W5KD30;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 2.
DT 27-MAR-2024, entry version 46.
DE RecName: Full=Glycogen debranching enzyme {ECO:0000256|ARBA:ARBA00020723};
DE EC=2.4.1.25 {ECO:0000256|ARBA:ARBA00012560};
DE EC=3.2.1.33 {ECO:0000256|ARBA:ARBA00012778};
DE AltName: Full=Glycogen debrancher {ECO:0000256|ARBA:ARBA00031477};
OS Astyanax mexicanus (Blind cave fish) (Astyanax fasciatus mexicanus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Characiformes;
OC Characoidei; Characidae; Astyanax.
OX NCBI_TaxID=7994 {ECO:0000313|Ensembl:ENSAMXP00000005491.2, ECO:0000313|Proteomes:UP000018467};
RN [1] {ECO:0000313|Proteomes:UP000018467}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=female {ECO:0000313|Proteomes:UP000018467};
RA Jeffery W., Warren W., Wilson R.K.;
RL Submitted (MAR-2013) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000018467}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=female {ECO:0000313|Proteomes:UP000018467};
RX PubMed=25329095; DOI=10.1038/ncomms6307;
RA McGaugh S.E., Gross J.B., Aken B., Blin M., Borowsky R., Chalopin D.,
RA Hinaux H., Jeffery W.R., Keene A., Ma L., Minx P., Murphy D., O'Quin K.E.,
RA Retaux S., Rohner N., Searle S.M., Stahl B.A., Tabin C., Volff J.N.,
RA Yoshizawa M., Warren W.C.;
RT "The cavefish genome reveals candidate genes for eye loss.";
RL Nat. Commun. 5:5307-5307(2014).
RN [3] {ECO:0000313|Ensembl:ENSAMXP00000005491.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-
CC alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase
CC in glycogen degradation. {ECO:0000256|ARBA:ARBA00003530}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in
CC glycogen phosphorylase limit dextrin.; EC=3.2.1.33;
CC Evidence={ECO:0000256|ARBA:ARBA00000927};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Transfers a segment of a (1->4)-alpha-D-glucan to a new
CC position in an acceptor, which may be glucose or a (1->4)-alpha-D-
CC glucan.; EC=2.4.1.25; Evidence={ECO:0000256|ARBA:ARBA00000439};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the glycogen debranching enzyme family.
CC {ECO:0000256|ARBA:ARBA00025780}.
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DR STRING; 7994.ENSAMXP00000005491; -.
DR Ensembl; ENSAMXT00000005491.2; ENSAMXP00000005491.2; ENSAMXG00000005334.2.
DR eggNOG; KOG3625; Eukaryota.
DR GeneTree; ENSGT00390000012596; -.
DR HOGENOM; CLU_001517_2_0_1; -.
DR InParanoid; W5KD30; -.
DR Proteomes; UP000018467; Unassembled WGS sequence.
DR Bgee; ENSAMXG00000005334; Expressed in muscle tissue and 14 other cell types or tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004134; F:4-alpha-glucanotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0004135; F:amylo-alpha-1,6-glucosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0102500; F:beta-maltose 4-alpha-glucanotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0005978; P:glycogen biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0005980; P:glycogen catabolic process; IEA:InterPro.
DR CDD; cd11327; AmyAc_Glg_debranch_2; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 2.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR010401; AGL/Gdb1.
DR InterPro; IPR032788; AGL_central.
DR InterPro; IPR029436; AGL_euk_N.
DR InterPro; IPR032792; AGL_glucanoTrfase.
DR InterPro; IPR032790; GDE_C.
DR InterPro; IPR006421; Glycogen_debranch_met.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR NCBIfam; TIGR01531; glyc_debranch; 1.
DR PANTHER; PTHR10569; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR10569:SF2; GLYCOGEN DEBRANCHING ENZYME; 1.
DR Pfam; PF06202; GDE_C; 1.
DR Pfam; PF14701; hDGE_amylase; 1.
DR Pfam; PF14702; hGDE_central; 1.
DR Pfam; PF14699; hGDE_N; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Glycogen biosynthesis {ECO:0000256|ARBA:ARBA00023056};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Reference proteome {ECO:0000313|Proteomes:UP000018467};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 31..116
FT /note="Eukaryotic glycogen debranching enzyme N-terminal"
FT /evidence="ECO:0000259|Pfam:PF14699"
FT DOMAIN 122..548
FT /note="Glycogen debranching enzyme glucanotransferase"
FT /evidence="ECO:0000259|Pfam:PF14701"
FT DOMAIN 694..944
FT /note="Glycogen debranching enzyme central"
FT /evidence="ECO:0000259|Pfam:PF14702"
FT DOMAIN 1043..1496
FT /note="Glycogen debranching enzyme C-terminal"
FT /evidence="ECO:0000259|Pfam:PF06202"
SQ SEQUENCE 1501 AA; 169820 MW; 623AE41BA412F0AE CRC64;
MSPSKQIRVL VLNDMEKLDR TLFRLEQGFE LQFRLGPTLQ GKNIHVHTNY PAQGEQFDRH
RFRTLDWINP TGREDDSDKF CSLELQIAGS YQYYFGQGDK DKSGGGYIVV DPMLRVGADN
HVLPLDCISI QTYLAKCLGP LDEWLDRLRV AKETGYNMIH FTPLQTLGES RSCYSLADQL
ELNPDFSPPG QNYTWTDVGN LVEKMKKEWD MLCITDVVYN HTAANSVWIQ KHPECGYNLL
NSPHLRPAWV LDRALWHVTC DIADGKYADR GVPALLQNEH HMNSIRGIFW QDVYPKTKLW
EFFQVKVEPL VEKFRTLLQN GDKIQTDGKK QLKIIQDPQY NRFGCTVDLN TALEIFVSNG
PGAIEDCCNW FRKRLEELNA EQFKEVNYHQ EQATNCCMGT VSYERLADHG PKLGPVTRKH
PLVTRYFTFP FKESPLEQDL LLMDQPDKAC HFLAHNGWVM GDDPLRNFAE PGSNVYIRRE
LICWGDSVKL RYGKGPEDCP YLWAHMQKYT EITAKYFTGV RLDNCHSTPL HVAEFMLAAA
RAINPDLYVV AELFTGSEEL DNVFVTRLGI SSLIREALSA GDSHEEGRLV YRYGGEPVGA
FLQPSLRPLV PSIAHAMFLD ITHDNECPIQ IRSAFDSLPS SAIVSMACCA TGSTRGYDEF
VPHQISVVSE ERFYQKWNPQ AKPSPPGEVS LQSGIIAGKL ALNRLHQELA AKGFSQVYVD
QVDEDIVAVT RHCPSSHQSV VAVCRTAFKN PKHHKYSDEV PPMFIPGQWL VQVQKVKNPL
HGFLKDSTVV KQAGVTSRGR SEFVQEIVFE RLTPGSVIAF RVSLDPTAQE LVGILRTHLI
QFNPHYAAGS IPDKNAPEIL KTPLKESKLT LADMNVLMFR CDEEEKEDGG GCYSIPGWTT
LKYGGLQGLL SVMADIRPKN DLGHPFCDNL RQGDWMIDYV SSRLVSHGGT LGEVGQWFQA
MFGYLKHIPR YLIPCYFDAV LVGAYTTALD ASFKLMSSFV QQGSSFVRQL ALGSIQMCGI
GRFPALPPLS PALTDVPYRT NSITQQEQQF CVSMAAGLPH FSAGIFRCWG RDTFIALRGL
LLVTGRHLEA RNIILSFAGT LRHGLIPNLL GEGVSARFNC RDAVWWWLQC IQDYSTIVPN
GTSILQCPVS RMYPTDDSQP QAPGTVDQPL YDVIHEALQR HMQGIEFRER NAGPQIDRNM
RDEGFNIVAK VNPDTGFVYG GNRFNCGTWM DKMGESERAR NKGIPATPRD GSAVEIVALC
KSAVRWLVEL HKQGLFPYDC VTINKDGAKI TVPYADWNRN LQNNFEKMFY VSQDPKDPNE
KHPELVHKRG IYKDSVGASS PWCDYQLRPN FTIAMVVAPE LFTVERAWEA LNMAEKLLGP
LGMKTLDPDD MVYCGVYDNS LDNDNYNLAK GFNYHQGPEW LWPVGYFLRA KLYFAKKLGK
ETYDKTVYLV KNVLSRHYVH LERSPWKGLP ELTNENGQYC SFSCETQAWS IATVLEVLYD
L
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