ID W5KL94_ASTMX Unreviewed; 1276 AA.
AC W5KL94;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 2.
DT 27-MAR-2024, entry version 68.
DE SubName: Full=Ras protein specific guanine nucleotide releasing factor 2 {ECO:0000313|Ensembl:ENSAMXP00000008356.2};
OS Astyanax mexicanus (Blind cave fish) (Astyanax fasciatus mexicanus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Characiformes;
OC Characoidei; Characidae; Astyanax.
OX NCBI_TaxID=7994 {ECO:0000313|Ensembl:ENSAMXP00000008356.2, ECO:0000313|Proteomes:UP000018467};
RN [1] {ECO:0000313|Proteomes:UP000018467}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=female {ECO:0000313|Proteomes:UP000018467};
RA Jeffery W., Warren W., Wilson R.K.;
RL Submitted (MAR-2013) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000018467}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=female {ECO:0000313|Proteomes:UP000018467};
RX PubMed=25329095; DOI=10.1038/ncomms6307;
RA McGaugh S.E., Gross J.B., Aken B., Blin M., Borowsky R., Chalopin D.,
RA Hinaux H., Jeffery W.R., Keene A., Ma L., Minx P., Murphy D., O'Quin K.E.,
RA Retaux S., Rohner N., Searle S.M., Stahl B.A., Tabin C., Volff J.N.,
RA Yoshizawa M., Warren W.C.;
RT "The cavefish genome reveals candidate genes for eye loss.";
RL Nat. Commun. 5:5307-5307(2014).
RN [3] {ECO:0000313|Ensembl:ENSAMXP00000008356.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
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DR AlphaFoldDB; W5KL94; -.
DR STRING; 7994.ENSAMXP00000008356; -.
DR Ensembl; ENSAMXT00000008356.2; ENSAMXP00000008356.2; ENSAMXG00000008134.2.
DR eggNOG; KOG3417; Eukaryota.
DR GeneTree; ENSGT00940000155679; -.
DR HOGENOM; CLU_003405_0_1_1; -.
DR InParanoid; W5KL94; -.
DR OrthoDB; 68574at2759; -.
DR Proteomes; UP000018467; Unassembled WGS sequence.
DR Bgee; ENSAMXG00000008134; Expressed in camera-type eye and 5 other cell types or tissues.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR CDD; cd13261; PH_RasGRF1_2; 1.
DR CDD; cd00155; RasGEF; 1.
DR CDD; cd06224; REM; 1.
DR CDD; cd00160; RhoGEF; 1.
DR Gene3D; 1.20.900.10; Dbl homology (DH) domain; 1.
DR Gene3D; 2.30.29.30; Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB); 2.
DR Gene3D; 1.10.840.10; Ras guanine-nucleotide exchange factors catalytic domain; 1.
DR Gene3D; 1.20.870.10; Son of sevenless (SoS) protein Chain: S domain 1; 2.
DR InterPro; IPR035899; DBL_dom_sf.
DR InterPro; IPR000219; DH-domain.
DR InterPro; IPR000048; IQ_motif_EF-hand-BS.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR008937; Ras-like_GEF.
DR InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR InterPro; IPR019804; Ras_G-nucl-exch_fac_CS.
DR InterPro; IPR023578; Ras_GEF_dom_sf.
DR InterPro; IPR001895; RASGEF_cat_dom.
DR InterPro; IPR036964; RASGEF_cat_dom_sf.
DR PANTHER; PTHR23113; GUANINE NUCLEOTIDE EXCHANGE FACTOR; 1.
DR PANTHER; PTHR23113:SF187; RAS-SPECIFIC GUANINE NUCLEOTIDE-RELEASING FACTOR 2; 1.
DR Pfam; PF00169; PH; 1.
DR Pfam; PF00617; RasGEF; 1.
DR Pfam; PF00618; RasGEF_N; 1.
DR Pfam; PF00621; RhoGEF; 1.
DR SMART; SM00233; PH; 2.
DR SMART; SM00147; RasGEF; 1.
DR SMART; SM00229; RasGEFN; 2.
DR SMART; SM00325; RhoGEF; 1.
DR SUPFAM; SSF48065; DBL homology domain (DH-domain); 1.
DR SUPFAM; SSF50729; PH domain-like; 2.
DR SUPFAM; SSF48366; Ras GEF; 1.
DR PROSITE; PS50010; DH_2; 1.
DR PROSITE; PS50096; IQ; 1.
DR PROSITE; PS50003; PH_DOMAIN; 2.
DR PROSITE; PS00720; RASGEF; 1.
DR PROSITE; PS50009; RASGEF_CAT; 1.
DR PROSITE; PS50212; RASGEF_NTER; 1.
PE 4: Predicted;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Guanine-nucleotide releasing factor {ECO:0000256|ARBA:ARBA00022658,
KW ECO:0000256|PROSITE-ProRule:PRU00168};
KW Reference proteome {ECO:0000313|Proteomes:UP000018467}.
FT DOMAIN 22..129
FT /note="PH"
FT /evidence="ECO:0000259|PROSITE:PS50003"
FT DOMAIN 239..425
FT /note="DH"
FT /evidence="ECO:0000259|PROSITE:PS50010"
FT DOMAIN 466..584
FT /note="PH"
FT /evidence="ECO:0000259|PROSITE:PS50003"
FT DOMAIN 631..756
FT /note="N-terminal Ras-GEF"
FT /evidence="ECO:0000259|PROSITE:PS50212"
FT DOMAIN 1041..1273
FT /note="Ras-GEF"
FT /evidence="ECO:0000259|PROSITE:PS50009"
FT REGION 733..849
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 874..911
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 760..807
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 822..839
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1276 AA; 145332 MW; BE5813C00CD34F46 CRC64;
MQKSVRYNEG HALYLSAVAR KEGTKRGFLS KKTTENSRWH DKYFALYQNV LFYFDTEQSA
RPSGIYLLEG CTCERVPAPK VSTGGKETLD KLHYFLIVFG HDAQKPLELR TEEEAECDEW
VEVIQQASYS DIIIEREVLM QKYIHLVQIV ETEKVAANQL RTQLEDQDTE IERLKAEIIA
LNKTKERMRP YQVYQEEEDP DIKKIKKVQS FMRGWLCRRK WKIIVQDYIC SPHAESMRKR
NQIVFNMVEA ETEYVHQLSI LVNCFLRPLR MAASSKKPPI SHDDVSSIFL NSETIMFLHE
IFHQGLKARI ANWPTLVLAD LFDILLPMLN IYQEFVRNHQ YSLQVLANCK QNRDFDKLLK
QYESNAACEG RMLETFLTYP MFQIPRYIIT LHELLAHTPH EHVERKSLEF AKSKLEELSR
VMHDEVSDTE NIRKNLAIER MIVEGCDILL DTSQTFVRQG SLIQLPSVER GKLSKVRLGS
LSLKKEGERQ CFLFTKHFLI CTRSSGGKLH LLKQGGVLSL IECTLIEEPD ASDEDSKTSG
QVFGHLDFKI VVEPADTPAF TVVLLAPSRQ EKAAWTSDIS QCIDNIRCNG LMTSVFEENS
KVTVPHMIKS DARLHKDDVD ICFSKTLNSC KVPQIRYASV ERLLERLTDL RFLSIDFLNT
FLHTYRIFTT ATVVMDKLAD IYKKPFTSIP VRVQYHSFDR LSITSICPDY SLKIRKIAMD
QSKSLELFFA TNQNNRTNSS GDHPNDKSPR LCRKFSSPPP LSIPSRTSSP VRTRKLSLNS
PLNSRVGALD LSSNPTSFSA ASSPTSSNPT TSPPPSNNPR SPPGQSQGQS PSSPEQSPGA
PEDNPDAPRI DALCKHRRSF RRAVLESVSL DKFIPESPQG SEAGDLSPCR SPSTPRHLRY
RGPGVPAAEN SRCSVSPASA FAIATAAAGH SSPPVFNNSE RTCDKEFIIR RAATNRVLNV
LRHWVSKHSQ DFELNTELKM GVMGLLEEVL RDPDLLPQER KATANILNAL SQDDQEDAQL
KIEDILQMAE CPKAECFESL SAMEIAEQIT LLDHIIFRSI PYEEFLGQGW MKMDKTERTP
YIMKTSQHFN DMSNLVASQI MSHTDVGSRA SSIEKWVAVA DICRCLNNYN GVLEITSALN
RSAIYRLKKT WAKISKQTKA LMDKLQKTVS SEGRFKNLRE TLKNCNPPSV PYLGMYLTDL
AFIEEGTPNF TEEGLVNFSK MRMISHIIRE IRQFQQTPYR IEHQPKVTQY LLDKALIMDE
DTLYDLSLKI EPRLPA
//
