ID W5NYL7_SHEEP Unreviewed; 978 AA.
AC W5NYL7;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 1.
DT 27-MAR-2024, entry version 51.
DE RecName: Full=formate--tetrahydrofolate ligase {ECO:0000256|ARBA:ARBA00012295};
DE EC=6.3.4.3 {ECO:0000256|ARBA:ARBA00012295};
GN Name=MTHFD1L {ECO:0000313|Ensembl:ENSOARP00000003265.1};
OS Ovis aries (Sheep).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Caprinae; Ovis.
OX NCBI_TaxID=9940 {ECO:0000313|Ensembl:ENSOARP00000003265.1, ECO:0000313|Proteomes:UP000002356};
RN [1] {ECO:0000313|Ensembl:ENSOARP00000003265.1, ECO:0000313|Proteomes:UP000002356}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Texel {ECO:0000313|Ensembl:ENSOARP00000003265.1,
RC ECO:0000313|Proteomes:UP000002356};
RX PubMed=20809919; DOI=10.1111/j.1365-2052.2010.02100.x;
RA Archibald A.L., Cockett N.E., Dalrymple B.P., Faraut T., Kijas J.W.,
RA Maddox J.F., McEwan J.C., Hutton Oddy V., Raadsma H.W., Wade C., Wang J.,
RA Wang W., Xun X.;
RT "The sheep genome reference sequence: a work in progress.";
RL Anim. Genet. 41:449-453(2010).
RN [2] {ECO:0000313|Ensembl:ENSOARP00000003265.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000256|ARBA:ARBA00004777}.
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the
CC formate--tetrahydrofolate ligase family.
CC {ECO:0000256|ARBA:ARBA00006985}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the tetrahydrofolate
CC dehydrogenase/cyclohydrolase family. {ECO:0000256|ARBA:ARBA00005559}.
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DR EMBL; AMGL01108368; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; W5NYL7; -.
DR SMR; W5NYL7; -.
DR STRING; 9940.ENSOARP00000003265; -.
DR PaxDb; 9940-ENSOARP00000003265; -.
DR Ensembl; ENSOART00000003326.1; ENSOARP00000003265.1; ENSOARG00000003065.1.
DR eggNOG; KOG4230; Eukaryota.
DR HOGENOM; CLU_003601_2_0_1; -.
DR OMA; KFWNLKC; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000002356; Chromosome 8.
DR Bgee; ENSOARG00000003065; Expressed in epididymis and 53 other cell types or tissues.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0004488; F:methylenetetrahydrofolate dehydrogenase (NADP+) activity; IEA:InterPro.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR CDD; cd05212; NAD_bind_m-THF_DH_Cyclohyd_like; 1.
DR Gene3D; 1.10.8.770; -; 1.
DR Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR Gene3D; 3.40.50.10860; Leucine Dehydrogenase, chain A, domain 1; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR046346; Aminoacid_DH-like_N_sf.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000672; THF_DH/CycHdrlase.
DR InterPro; IPR020630; THF_DH/CycHdrlase_cat_dom.
DR InterPro; IPR020631; THF_DH/CycHdrlase_NAD-bd_dom.
DR PANTHER; PTHR48099; C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED; 1.
DR PANTHER; PTHR48099:SF12; MONOFUNCTIONAL C1-TETRAHYDROFOLATE SYNTHASE, MITOCHONDRIAL; 1.
DR Pfam; PF01268; FTHFS; 1.
DR Pfam; PF00763; THF_DHG_CYH; 1.
DR Pfam; PF02882; THF_DHG_CYH_C; 1.
DR PRINTS; PR00085; THFDHDRGNASE.
DR SUPFAM; SSF53223; Aminoacid dehydrogenase-like, N-terminal domain; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 3: Inferred from homology;
KW One-carbon metabolism {ECO:0000256|ARBA:ARBA00022563};
KW Reference proteome {ECO:0000313|Proteomes:UP000002356}.
FT DOMAIN 83..181
FT /note="Tetrahydrofolate dehydrogenase/cyclohydrolase
FT catalytic"
FT /evidence="ECO:0000259|Pfam:PF00763"
FT DOMAIN 185..336
FT /note="Tetrahydrofolate dehydrogenase/cyclohydrolase
FT NAD(P)-binding"
FT /evidence="ECO:0000259|Pfam:PF02882"
FT REGION 13..69
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 978 AA; 105431 MW; 7397FE3F68C522BF CRC64;
MSALLPLVLR RLARPQHPGP PRRLPSLCRP SSSGGSGCGS GEGLLGQQRL PDTQAGSSRG
PSSPAPPARD SIVRQCREII QNSKEVLSLL QEKTPTFKPV LAVIQAGDDN LMQEVNQNLA
EEAGLNITHI CLPAESGEDE IIDEILKINE DSRVHGLALQ IAETSLSSKI LNALKPEKDV
DGLTDVNLGK LVRGDAHECF ISPVARAVIE LLEKSGVSLD GKKILVIGAH GSLEATLQCL
FQRKGSMTMS SQWKTPQLQG KLQEADIVVL GSPKPEEIPL SWIQPGTTIF NCSHDFLSGK
AACVSPGIHG ISPIAEDVSL LAAALRIQNM VSSGRRWLRE QQHRRWRLHC LKLQPLSPVP
SDIEISRAQT PKAVEILAKE IGLFADEIEI YGKSKAKVRL SLLERLKDQA DGKYVLVAGI
TPTPLGEGKS TVTIGLVQAL TAHLNVNSFA CLRQPSQGPT FGVKGGAAGG GYAQVIPMEE
FNLHLTGDIH AITAANNLLA AAIDARILHE NTQTDKALYN RLVPSVNGVR EFSKIQLARL
KRLGINKTDP SALTEEEMRK FARLDIDPST ITWQRVVDTN DRFLRKITIG QASTEKGCSR
QAQFDIAVAS EIMAVLALTD SLADMKERLG RMVVASDKNG QPVTADDLGV TGALTVLMKD
AIKPNLMQTL EGTPVFVHAG PFANIAHGNS SVLADKIALK LVGEGGFVVT EAGFGADIGM
EKFFNIKCRA SGLVPNVVVL VATVRALKMH GGGPSVTAGV PLKKEYTEEN LQLVADGCCN
LEKQIQIAQL FGVPVVVALN VFKTDTRAEI DLVCELAKRA GAFNAVPCYH WSIGGKGSVD
LAWAVREAAS KESRFQFLYD VQLPIVEKIR TIAQSVYGAK DIELSPEAQS KIDRYTEQGF
GNLPICMAKT HLSLSHQPDK KGVPKGFILP ISDVRASIGA GFIYPLVGTM STMPGLPTRP
CFYDIDLDTE TEQVKGLF
//
