ID W5P0X1_SHEEP Unreviewed; 418 AA.
AC W5P0X1;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 1.
DT 27-MAR-2024, entry version 56.
DE RecName: Full=Arylacetamide deacetylase {ECO:0000256|ARBA:ARBA00044154};
DE EC=3.1.1.3 {ECO:0000256|ARBA:ARBA00013279};
GN Name=LOC101115927 {ECO:0000313|Ensembl:ENSOARP00000004070.1};
OS Ovis aries (Sheep).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Caprinae; Ovis.
OX NCBI_TaxID=9940 {ECO:0000313|Ensembl:ENSOARP00000004070.1, ECO:0000313|Proteomes:UP000002356};
RN [1] {ECO:0000313|Ensembl:ENSOARP00000004070.1, ECO:0000313|Proteomes:UP000002356}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Texel {ECO:0000313|Ensembl:ENSOARP00000004070.1,
RC ECO:0000313|Proteomes:UP000002356};
RX PubMed=20809919; DOI=10.1111/j.1365-2052.2010.02100.x;
RA Archibald A.L., Cockett N.E., Dalrymple B.P., Faraut T., Kijas J.W.,
RA Maddox J.F., McEwan J.C., Hutton Oddy V., Raadsma H.W., Wade C., Wang J.,
RA Wang W., Xun X.;
RT "The sheep genome reference sequence: a work in progress.";
RL Anim. Genet. 41:449-453(2010).
RN [2] {ECO:0000313|Ensembl:ENSOARP00000004070.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid +
CC H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.3;
CC Evidence={ECO:0000256|ARBA:ARBA00001024};
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000256|ARBA:ARBA00004648}; Single-pass type II membrane protein
CC {ECO:0000256|ARBA:ARBA00004648}. Membrane
CC {ECO:0000256|ARBA:ARBA00004606}; Single-pass type II membrane protein
CC {ECO:0000256|ARBA:ARBA00004606}. Microsome membrane
CC {ECO:0000256|ARBA:ARBA00004464}; Single-pass type II membrane protein
CC {ECO:0000256|ARBA:ARBA00004464}.
CC -!- SIMILARITY: Belongs to the 'GDXG' lipolytic enzyme family.
CC {ECO:0000256|ARBA:ARBA00010515}.
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DR EMBL; AMGL01010817; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_004003293.1; XM_004003244.3.
DR AlphaFoldDB; W5P0X1; -.
DR STRING; 9940.ENSOARP00000004070; -.
DR ESTHER; sheep-w5p0x1; Arylacetamide_deacetylase.
DR MEROPS; S09.991; -.
DR PaxDb; 9940-ENSOARP00000004070; -.
DR Ensembl; ENSOART00000004142.1; ENSOARP00000004070.1; ENSOARG00000003815.1.
DR GeneID; 101115927; -.
DR KEGG; oas:101115927; -.
DR eggNOG; KOG1515; Eukaryota.
DR HOGENOM; CLU_012494_12_0_1; -.
DR OMA; PKHMARW; -.
DR OrthoDB; 1144477at2759; -.
DR Proteomes; UP000002356; Chromosome 1.
DR Bgee; ENSOARG00000003815; Expressed in uterus and 51 other cell types or tissues.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0052689; F:carboxylic ester hydrolase activity; IEA:InterPro.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR013094; AB_hydrolase_3.
DR InterPro; IPR017157; Arylacetamide_deacetylase.
DR InterPro; IPR002168; Lipase_GDXG_HIS_AS.
DR PANTHER; PTHR23024; ARYLACETAMIDE DEACETYLASE; 1.
DR PANTHER; PTHR23024:SF222; ARYLACETAMIDE DEACETYLASE; 1.
DR Pfam; PF07859; Abhydrolase_3; 2.
DR PIRSF; PIRSF037251; Arylacetamide_deacetylase; 1.
DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
DR PROSITE; PS01173; LIPASE_GDXG_HIS; 1.
PE 3: Inferred from homology;
KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157};
KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Microsome {ECO:0000256|ARBA:ARBA00022848};
KW Reference proteome {ECO:0000313|Proteomes:UP000002356};
KW Signal-anchor {ECO:0000256|ARBA:ARBA00022968};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 21..40
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 127..280
FT /note="Alpha/beta hydrolase fold-3"
FT /evidence="ECO:0000259|Pfam:PF07859"
FT DOMAIN 333..394
FT /note="Alpha/beta hydrolase fold-3"
FT /evidence="ECO:0000259|Pfam:PF07859"
FT ACT_SITE 209
FT /evidence="ECO:0000256|PIRSR:PIRSR037251-1"
FT ACT_SITE 362
FT /evidence="ECO:0000256|PIRSR:PIRSR037251-1"
FT ACT_SITE 392
FT /evidence="ECO:0000256|PIRSR:PIRSR037251-1"
SQ SEQUENCE 418 AA; 48008 MW; 4F39C6CE698BE4C0 CRC64;
MKFQHSNISA AKKNVQLENI MGRKTILLLI VGVLGAYYVY TPLPDNIEEP WKLMWVTTCL
KTMTHLCHFA ELLGISHFMD IARFFMSIQE VPPTSDENVT VMETTFNNVP VRIYLPKRKS
ETLRRGLFYI HGGGWHMGSA AFFDIDFLSR RTAVILDAVV ISTNYRLAPE HHFPSQFEDV
YNALKWFLNQ DVLDKYSVDP ERIGISGESA GGNLAAAVTQ QLIDDPDVKI KLKTQSLIYP
ALQCLDLDLP SYRENSNLGL TKSFVARLWS GYFTTDRSLE KAMFFNQHVP VESSHLFKFV
NWSSFLPEKF RKGHFYKSPT YGNSKLAKKY PGFLDVRAAP LLADDNRLRN LPLTYVITCQ
YDILRDDGIM YVTRLQNAGV RVTHNHIEDG FHGALVYRGF KIGYRIEKQY MSWLSENL
//
