ID W5PIX3_SHEEP Unreviewed; 184 AA.
AC W5PIX3;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 1.
DT 27-MAR-2024, entry version 41.
DE RecName: Full=Cytochrome-b5 reductase {ECO:0008006|Google:ProtNLM};
OS Ovis aries (Sheep).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Caprinae; Ovis.
OX NCBI_TaxID=9940 {ECO:0000313|Ensembl:ENSOARP00000010393.1, ECO:0000313|Proteomes:UP000002356};
RN [1] {ECO:0000313|Ensembl:ENSOARP00000010393.1, ECO:0000313|Proteomes:UP000002356}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Texel {ECO:0000313|Ensembl:ENSOARP00000010393.1,
RC ECO:0000313|Proteomes:UP000002356};
RX PubMed=20809919; DOI=10.1111/j.1365-2052.2010.02100.x;
RA Archibald A.L., Cockett N.E., Dalrymple B.P., Faraut T., Kijas J.W.,
RA Maddox J.F., McEwan J.C., Hutton Oddy V., Raadsma H.W., Wade C., Wang J.,
RA Wang W., Xun X.;
RT "The sheep genome reference sequence: a work in progress.";
RL Anim. Genet. 41:449-453(2010).
RN [2] {ECO:0000313|Ensembl:ENSOARP00000010393.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 Fe(III)-[cytochrome b5] + NADH = 2 Fe(II)-[cytochrome b5] +
CC H(+) + NAD(+); Xref=Rhea:RHEA:46680, Rhea:RHEA-COMP:10438, Rhea:RHEA-
CC COMP:10439, ChEBI:CHEBI:15378, ChEBI:CHEBI:29033, ChEBI:CHEBI:29034,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.6.2.2;
CC Evidence={ECO:0000256|ARBA:ARBA00029341};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974};
CC -!- SIMILARITY: Belongs to the flavoprotein pyridine nucleotide cytochrome
CC reductase family. {ECO:0000256|ARBA:ARBA00006105}.
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DR EMBL; AMGL01072944; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; W5PIX3; -.
DR SMR; W5PIX3; -.
DR STRING; 9940.ENSOARP00000010393; -.
DR PaxDb; 9940-ENSOARP00000010393; -.
DR Ensembl; ENSOART00000010548.1; ENSOARP00000010393.1; ENSOARG00000009692.1.
DR eggNOG; KOG0534; Eukaryota.
DR HOGENOM; CLU_003827_9_4_1; -.
DR OMA; SDATENW; -.
DR Proteomes; UP000002356; Chromosome 26.
DR Bgee; ENSOARG00000009692; Expressed in gastric lymph node and 53 other cell types or tissues.
DR GO; GO:0004128; F:cytochrome-b5 reductase activity, acting on NAD(P)H; IEA:UniProt.
DR CDD; cd06183; cyt_b5_reduct_like; 1.
DR Gene3D; 3.40.50.80; Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module; 1.
DR InterPro; IPR001834; CBR-like.
DR InterPro; IPR008333; Cbr1-like_FAD-bd_dom.
DR InterPro; IPR039261; FNR_nucleotide-bd.
DR InterPro; IPR001433; OxRdtase_FAD/NAD-bd.
DR InterPro; IPR017938; Riboflavin_synthase-like_b-brl.
DR PANTHER; PTHR19370; NADH-CYTOCHROME B5 REDUCTASE; 1.
DR PANTHER; PTHR19370:SF185; NADH-CYTOCHROME B5 REDUCTASE 2; 1.
DR Pfam; PF00970; FAD_binding_6; 1.
DR Pfam; PF00175; NAD_binding_1; 1.
DR SUPFAM; SSF52343; Ferredoxin reductase-like, C-terminal NADP-linked domain; 1.
DR SUPFAM; SSF63380; Riboflavin synthase domain-like; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000002356}.
FT DOMAIN 4..74
FT /note="Flavoprotein pyridine nucleotide cytochrome
FT reductase-like FAD-binding"
FT /evidence="ECO:0000259|Pfam:PF00970"
FT DOMAIN 65..163
FT /note="Oxidoreductase FAD/NAD(P)-binding"
FT /evidence="ECO:0000259|Pfam:PF00175"
SQ SEQUENCE 184 AA; 21029 MW; 249F01AF71D9C7CC CRC64;
MLPPSTHLQV PVRQHVYLKL PITGTESVKP YTPVSDSLFS EFKEPVLPNN IYIYFLIKIY
PAGFFTPELD HLQIALTNIP SLRKVKLMFF NKTEDDIIWR SQLEKLACKD KRFEVEFVLS
APTSEWSGKQ GYISLALLSE FLKRSSDTSK VLICLCGPTP FTEQGMKMLH DLNFSKDEIH
SSTA
//
