ID W5PT16_SHEEP Unreviewed; 607 AA.
AC W5PT16;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 1.
DT 27-MAR-2024, entry version 41.
DE RecName: Full=Protein-S-isoprenylcysteine O-methyltransferase {ECO:0000256|ARBA:ARBA00023656, ECO:0000256|RuleBase:RU362022};
DE EC=2.1.1.100 {ECO:0000256|RuleBase:RU362022};
OS Ovis aries (Sheep).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Caprinae; Ovis.
OX NCBI_TaxID=9940 {ECO:0000313|Ensembl:ENSOARP00000013596.1, ECO:0000313|Proteomes:UP000002356};
RN [1] {ECO:0000313|Ensembl:ENSOARP00000013596.1, ECO:0000313|Proteomes:UP000002356}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Texel {ECO:0000313|Ensembl:ENSOARP00000013596.1,
RC ECO:0000313|Proteomes:UP000002356};
RX PubMed=20809919; DOI=10.1111/j.1365-2052.2010.02100.x;
RA Archibald A.L., Cockett N.E., Dalrymple B.P., Faraut T., Kijas J.W.,
RA Maddox J.F., McEwan J.C., Hutton Oddy V., Raadsma H.W., Wade C., Wang J.,
RA Wang W., Xun X.;
RT "The sheep genome reference sequence: a work in progress.";
RL Anim. Genet. 41:449-453(2010).
RN [2] {ECO:0000313|Ensembl:ENSOARP00000013596.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Catalyzes the post-translational methylation of isoprenylated
CC C-terminal cysteine residues. {ECO:0000256|ARBA:ARBA00023572}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine + S-
CC adenosyl-L-methionine = [protein]-C-terminal S-[(2E,6E)-farnesyl]-L-
CC cysteine methyl ester + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:21672, Rhea:RHEA-COMP:12125, Rhea:RHEA-COMP:12126,
CC ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:90510,
CC ChEBI:CHEBI:90511; EC=2.1.1.100;
CC Evidence={ECO:0000256|ARBA:ARBA00001450,
CC ECO:0000256|RuleBase:RU362022};
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000256|RuleBase:RU362022}; Multi-pass membrane protein
CC {ECO:0000256|RuleBase:RU362022}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the class VI-like SAM-binding methyltransferase
CC superfamily. Isoprenylcysteine carboxyl methyltransferase family.
CC {ECO:0000256|RuleBase:RU362022}.
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DR EMBL; AMGL01021756; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AMGL01021757; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AMGL01021758; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AMGL01021759; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; W5PT16; -.
DR SMR; W5PT16; -.
DR STRING; 9940.ENSOARP00000013596; -.
DR PaxDb; 9940-ENSOARP00000013596; -.
DR Ensembl; ENSOART00000013798.1; ENSOARP00000013596.1; ENSOARG00000012693.1.
DR eggNOG; KOG2628; Eukaryota.
DR HOGENOM; CLU_031380_0_0_1; -.
DR OMA; HTDLMYE; -.
DR Proteomes; UP000002356; Chromosome 12.
DR Bgee; ENSOARG00000012693; Expressed in cerebellum and 55 other cell types or tissues.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW.
DR GO; GO:0004671; F:protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR Gene3D; 1.20.120.1630; -; 1.
DR Gene3D; 1.20.1070.10; Rhodopsin 7-helix transmembrane proteins; 1.
DR InterPro; IPR022347; GCR_153/162.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR022335; GPR153.
DR InterPro; IPR007269; ICMT_MeTrfase.
DR InterPro; IPR025770; PPMT_MeTrfase.
DR PANTHER; PTHR16518; G-PROTEIN COUPLED RECEPTOR 153, 162; 1.
DR PANTHER; PTHR16518:SF5; G-PROTEIN COUPLED RECEPTOR 153-RELATED; 1.
DR Pfam; PF00001; 7tm_1; 1.
DR Pfam; PF04140; ICMT; 1.
DR PRINTS; PR01992; GPR153.
DR PRINTS; PR01991; GPR153GPR162.
DR SUPFAM; SSF81321; Family A G protein-coupled receptor-like; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
DR PROSITE; PS51564; SAM_ICMT; 1.
PE 3: Inferred from homology;
KW Endoplasmic reticulum {ECO:0000256|RuleBase:RU362022};
KW G-protein coupled receptor {ECO:0000256|ARBA:ARBA00023040};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU362022};
KW Methyltransferase {ECO:0000256|RuleBase:RU362022};
KW Receptor {ECO:0000256|ARBA:ARBA00023040};
KW Reference proteome {ECO:0000313|Proteomes:UP000002356};
KW S-adenosyl-L-methionine {ECO:0000256|RuleBase:RU362022};
KW Transducer {ECO:0000256|ARBA:ARBA00023040};
KW Transferase {ECO:0000256|RuleBase:RU362022};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW ECO:0000256|RuleBase:RU362022};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|RuleBase:RU362022}.
FT TRANSMEM 12..32
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362022"
FT TRANSMEM 44..65
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362022"
FT TRANSMEM 85..106
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362022"
FT TRANSMEM 127..150
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362022"
FT TRANSMEM 156..180
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362022"
FT TRANSMEM 241..264
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362022"
FT TRANSMEM 276..298
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362022"
FT TRANSMEM 389..410
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362022"
FT DOMAIN 24..142
FT /note="G-protein coupled receptors family 1 profile"
FT /evidence="ECO:0000259|PROSITE:PS50262"
SQ SEQUENCE 607 AA; 67697 MW; E9BCF18BF23BC5F4 CRC64;
RGEGRRLPGS AVGWLACGGL SLLANAWGIL SVGAKQKKWK PLEFLLCTLA ATHMLNVAVP
IATYAVVQLR RQRPDYEWNE GLCKVFVSTF YTLTLATCFS VTSLSYHRMW MVRWPVNYRL
SNAKKQAVHT VMGIWMVSFI LSALPAVGWH DTSERFYTHG CRFIVAEIGL GFGVCFLLLV
GGSVAMGAGP GGGAPPPPPR GGGGGAARRA FTVPTIVVED AQGKRRSSID GSEPARTSLQ
ITGLVATIVV IYDCLMGFPV LVVSFSSLRA DASAPWMALC VLWCSVTQAL LLPVFLWACD
RYRADLKAVW EKCVALMAND EDSDDDTSLE RGVPPDLVLE RSLDYSYGGD FVALERMAKY
ELSALEEGGL PQFYPLRPLQ EDKMQYLQIA IRACFLGFVF GCGVLLSFSQ SSWNHFGWYM
CSLSLFHYSE YLVTAVNNPK SLSLDSFLLN HSLEYTVAAL SSWIEFTLEN IFWPELKQIT
WISATGLLMV VFGECLRKAA MFTAGSNFNH VVQNEKSETH TLVTSGVYAW FRHPSYVGWF
YWSIGTQVML CNPICGVSYA LTVWRFFRDR TEEEEISLIH FFGEEYLEYK KRVPTGLPFI
KGVKVEL
//
