ID W6K1M4_9MICO Unreviewed; 275 AA.
AC W6K1M4;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE SubName: Full=2,5-diketo-D-gluconic acid reductase B {ECO:0000313|EMBL:CCH75382.1};
DE EC=1.1.1.274 {ECO:0000313|EMBL:CCH75382.1};
GN Name=dkgB {ECO:0000313|EMBL:CCH75382.1};
GN ORFNames=BN11_660020 {ECO:0000313|EMBL:CCH75382.1};
OS Tetrasphaera australiensis Ben110.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Intrasporangiaceae;
OC Tetrasphaera.
OX NCBI_TaxID=1193182 {ECO:0000313|EMBL:CCH75382.1, ECO:0000313|Proteomes:UP000035763};
RN [1] {ECO:0000313|EMBL:CCH75382.1, ECO:0000313|Proteomes:UP000035763}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ben110 {ECO:0000313|EMBL:CCH75382.1,
RC ECO:0000313|Proteomes:UP000035763};
RX PubMed=23178666; DOI=10.1038/ismej.2012.136;
RA Kristiansen R., Nguyen H.T.T., Saunders A.M., Nielsen J.L., Wimmer R.,
RA Le V.Q., McIlroy S.J., Petrovski S., Seviour R.J., Calteau A.,
RA Nielsen K.L., Nielsen P.H.;
RT "A metabolic model for members of the genus Tetrasphaera involved in
RT enhanced biological phosphorus removal.";
RL ISME J. 7:543-554(2013).
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCH75382.1}.
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DR EMBL; CAJA01000492; CCH75382.1; -; Genomic_DNA.
DR RefSeq; WP_048695823.1; NZ_HG764815.1.
DR AlphaFoldDB; W6K1M4; -.
DR STRING; 1193182.BN11_660020; -.
DR OrthoDB; 9804790at2; -.
DR Proteomes; UP000035763; Unassembled WGS sequence.
DR GO; GO:0050580; F:2,5-didehydrogluconate reductase activity; IEA:UniProtKB-EC.
DR GO; GO:0071704; P:organic substance metabolic process; IEA:UniProt.
DR GO; GO:0044281; P:small molecule metabolic process; IEA:UniProt.
DR CDD; cd19132; AKR_AKR5D1_E1; 1.
DR Gene3D; 3.20.20.100; NADP-dependent oxidoreductase domain; 1.
DR InterPro; IPR020471; AKR.
DR InterPro; IPR018170; Aldo/ket_reductase_CS.
DR InterPro; IPR023210; NADP_OxRdtase_dom.
DR InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR PANTHER; PTHR43827; 2,5-DIKETO-D-GLUCONIC ACID REDUCTASE; 1.
DR PANTHER; PTHR43827:SF3; ALDO-KETO REDUCTASE; 1.
DR Pfam; PF00248; Aldo_ket_red; 1.
DR PIRSF; PIRSF000097; AKR; 2.
DR PRINTS; PR00069; ALDKETRDTASE.
DR SUPFAM; SSF51430; NAD(P)-linked oxidoreductase; 1.
DR PROSITE; PS00798; ALDOKETO_REDUCTASE_1; 1.
DR PROSITE; PS00062; ALDOKETO_REDUCTASE_2; 1.
PE 4: Predicted;
KW Oxidoreductase {ECO:0000313|EMBL:CCH75382.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000035763}.
FT DOMAIN 14..257
FT /note="NADP-dependent oxidoreductase"
FT /evidence="ECO:0000259|Pfam:PF00248"
FT ACT_SITE 47
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000097-1"
FT BINDING 105
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000097-2"
FT SITE 72
FT /note="Lowers pKa of active site Tyr"
FT /evidence="ECO:0000256|PIRSR:PIRSR000097-3"
SQ SEQUENCE 275 AA; 30020 MW; E6049F9FB1F27350 CRC64;
MNTALRDGAA IPEIGFGTSP LKGEDAIRAV GSAIEAGYRL IDTAVNYENE SEVGEAIRRS
EVERAEFVVQ SKIPGRDHAY DRAVASVRGS LSRLGLDQLD VGLIHWPNPS VDLYADAWRA
LVDLQRDGLV RVIGVSNFHP HHLARIVDVT GVVPAINQVE MHPYFPQFAL RAEHERLGII
TEAWSPLGKG MNAYGEAPVR DAAAAHGVSP AQAILRWHVQ LGSVPLPKSE NPQRQRENLD
VYGFTLTDDE LAAITGLARA DGRRFGGDPD THEEM
//
