ID W7FK52_PLAFA Unreviewed; 1480 AA.
AC W7FK52;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE RecName: Full=leucine--tRNA ligase {ECO:0000256|ARBA:ARBA00013164};
DE EC=6.1.1.4 {ECO:0000256|ARBA:ARBA00013164};
DE AltName: Full=Leucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00030520};
GN ORFNames=PFAG_02179 {ECO:0000313|EMBL:EUT87357.1};
OS Plasmodium falciparum Santa Lucia.
OC Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC Plasmodiidae; Plasmodium; Plasmodium (Laverania).
OX NCBI_TaxID=478859 {ECO:0000313|EMBL:EUT87357.1};
RN [1] {ECO:0000313|EMBL:EUT87357.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Santa Lucia {ECO:0000313|EMBL:EUT87357.1};
RG The Broad Institute Genome Sequencing Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Neafsey D., Cheeseman I., Volkman S., Adams J., Walker B., Young S.K.,
RA Zeng Q., Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Alvarado L.,
RA Arachchi H.M., Berlin A.M., Chapman S.B., Dewar J., Goldberg J., Griggs A.,
RA Gujja S., Hansen M., Howarth C., Imamovic A., Larimer J., McCowan C.,
RA Murphy C., Neiman D., Pearson M., Priest M., Roberts A., Saif S., Shea T.,
RA Sisk P., Sykes S., Wortman J., Nusbaum C., Birren B.;
RT "The Genome Sequence of Plasmodium falciparum Santa Lucia.";
RL Submitted (FEB-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00001372};
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594, ECO:0000256|RuleBase:RU363035}.
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DR EMBL; KE123491; EUT87357.1; -; Genomic_DNA.
DR EnsemblProtists; EUT87357; EUT87357; PFAG_02179.
DR Proteomes; UP000030666; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:InterPro.
DR Gene3D; 3.40.50.620; HUPs; 3.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR002302; Leu-tRNA-ligase.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR PANTHER; PTHR43740:SF2; LEUCINE--TRNA LIGASE, MITOCHONDRIAL; 1.
DR PANTHER; PTHR43740; LEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 3.
DR PRINTS; PR00985; TRNASYNTHLEU.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 2.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|RuleBase:RU363035};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363035};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363035};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363035};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW ECO:0000256|RuleBase:RU363035}; Signal {ECO:0000256|SAM:SignalP};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT SIGNAL 1..27
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 28..1480
FT /note="leucine--tRNA ligase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5004895032"
FT TRANSMEM 370..392
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 625..650
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 121..325
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 669..750
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 1118..1155
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 1316..1407
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
SQ SEQUENCE 1480 AA; 178288 MW; F8EFCAAA8AADB029 CRC64;
MFFKFVRFLI YIFLYSLCYQ HVNNVFALHI NKYKNVFLFE SPSVLIKNRR NYKRKIYSHD
EEKGKYDFHR IEEKWQTIWN SKRLLDKDFE QYNKINLGRK EKKEKRHKDK QNGFIDKPYN
DVYEKGDNTY KYNKKKFYIL DMFPYPSSEG LHVGHILCFT ITDIISKFKK MNNYCVFHPI
GWDSFGLPCD RLSMKKKIDP REIIHKNISN FKNQLIKLGF LFNWESEINT CDENYYKWTQ
WIIIQLFLNN LSYKKRSYVN WSNELRCVIS NDELRNEINL QYLNIQKIKL LQWYLKITKY
ANRLIKDLNM IHWPMKIKNM QINWIGKKTG IFLKARIISL DKFLNNTHFN FVNIKKSYNN
NMINVFYNNI FNHLFFIFIN YILSIFTFHM YINNPNEQKQ QQKKNTHIKE LNNKIQKYNY
INKYDFFFSY HQNDLFLFFS FFFNYYHNRN IDQMKKQKLN LPTEPFKNEQ IRVSHNKENN
NNSSNLETNW VSFNIQNCND MIINILFNNM ETYNENYFLY PLKDATKGKN YINGHNSNDN
MFNDNMFNDN MFDDNMFDDN MLKDQNSYVQ IFLNKNEVIL QNDKLIISVN HPNIRNIVNH
NPFLLKFIDK MVLENDTVRL KNEELYFTGS VIYFPIMNKM IPVFLCAYIL DNNKNFLFLK
KEMTQTQDKN SNTNISLLYK MLQDSNYSKK KNIYNLKDWL FSRQRYWGEP FPFLYKMKDK
NGYMDNTKIK NDDHMNENNH NNNMLSDCKD INNFSNYNDQ NNNKKIRIRK VINSPNNNIY
IDDIPLKLPN FNKKIYEIDP HNEQDVNNIS SVLSRFKKWM ITKKYNMLYK RESDIMPQWA
GSSWYFLRYI DSKNKKRIFN KKKINSWLPV DLYVGGSEHA VLHLLYSRFF HKFLYDLKLT
KHKEPFQKLF NQGLLLNTTS FYLYTTLDNK PVSFDQINEK KINTNAYDYT NRGTNHHIQL
NKKENEKKKK TYSQNVCETT NLLTSQVLPD HASFCSGDQN GNINKELKNE RNENMKEKDI
DILRKSKIND DMKEKAIDML GKKINDDMKE KAIDMLGKNK INDDMKENVI DMLGKNNYTI
VDNKYKKFLI EEDYVKEGKD QKYYLKSFPS IEVQPNYEKM SKSKGNTINP LDIVKTYGSD
CLRLHILFLG PVDQNKKWTT KGIKGTFKFL NNLYNLFIQR CDIKNNKESN KKNVCTRVVD
NSMNDMMFHK DNIEKSANHI LSNNGYEKKK KINLENGEKK IEYMICKNCK RKSSNKKLIL
NFLKNKYGFI IANEKYKKVN DYVNIILKND SHVFNKLSDK IKKIKIEDIE NEKKKKVNYY
IEKITNCIND IKLNTAVSFF MKFYNEIKTW DIVPLKIFII FVKLLYPFCP HICEEFWFYY
LKRYKIKKRK KLCYFCNSNL LYYGKWPSLF EIKQNKLVNI SIKLNNKHIT FLQRDVSSTS
DITEEATNLI KHKIENEMKK GKKIVNIVNI PNKVINFIIK
//
