ID W7P6P1_9ENTR Unreviewed; 1148 AA.
AC W7P6P1;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 1.
DT 24-JAN-2024, entry version 43.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969,
GN ECO:0000313|EMBL:EWG77835.1};
GN ORFNames=P349_00636 {ECO:0000313|EMBL:EWG77835.1};
OS Enterobacter sp. DC4.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Enterobacter.
OX NCBI_TaxID=1395580 {ECO:0000313|EMBL:EWG77835.1, ECO:0000313|Proteomes:UP000019283};
RN [1] {ECO:0000313|EMBL:EWG77835.1, ECO:0000313|Proteomes:UP000019283}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DC4 {ECO:0000313|EMBL:EWG77835.1};
RX PubMed=24625871;
RA Gan H.M., Triassi A.J., Wheatley M.S., Savka M.A., Hudson A.O.;
RT "High-Quality Draft Whole-Genome Sequences of Three Strains of Enterobacter
RT Isolated from Jamaican Dioscorea cayenensis (Yellow Yam).";
RL Genome Announc. 2:e00170-14(2014).
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EWG77835.1}.
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DR EMBL; AZUB01000001; EWG77835.1; -; Genomic_DNA.
DR RefSeq; WP_032650865.1; NZ_AZUB01000001.1.
DR AlphaFoldDB; W7P6P1; -.
DR PATRIC; fig|1395580.4.peg.625; -.
DR Proteomes; UP000019283; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR CDD; cd18810; SF2_C_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11140; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR048635; MFD_D3.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF21132; MFD_D3; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}.
FT DOMAIN 615..776
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 798..951
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1148 AA; 130009 MW; 19BE4D9BEE86AD94 CRC64;
MPEHYRYSLP VKAGDQRQLG ELTGAACATL VAEIAERHPG PVILVAPDMQ NALRLHDEIR
QFTDNLVFSL ADWETLPYDS FSPHQEIISS RLSTLYQLPT MQRGVLIVPV NTLMQRVCPH
SYLHGHALVM KKGQRLSRDA LRAQLDGAGY RHVDQVMEHG EYATRGALLD LYPMGSDQPY
RLDFFDDEID SLRVFDADTQ RTLEEVESIN LLPAHEFPTD KTAIELFRSQ WRDKFEVKRD
AEHIYQQVSK GTLPAGIEYW QPLFFNEPLP ALFSYFPANT LIVNTGDIDA SASRFESETR
ARFENRGVDP MRPLLPPEAL WLRTDELNAE LKRWPRMQLK TDSLADKAAN TNLAFRTLPD
LAVQAQQKSP LDSLRKFLES FTGPVVFSVE SEGRREALGE LLGRIKVAPK RILRLSEATG
NGRYLMIGAA EHGFIDTLNN LALICESDLL GERVARRRQD SRRTINPDTL IRNLAELHPG
QPIVHLEHGV GRYQGMTTLE AGGIKGEYLM LTYANDAKLY VPVSSLHLIS RYAGGAEENA
PLHKLGGDAW ARARQKAAEK VRDVAAELLD IYAQRAAKEG FAFKHDKEQY QLFCDSFPFE
TTPDQAQAIN AVLSDMCQPL AMDRLVCGDV GFGKTEVAMR AAFLAVENNK QVAVLVPTTL
LAQQHFDNFR DRFANWPVRI EMLSRFRSTK EQTQILEQAS EGKIDILIGT HKLLQSDVKW
KDLGLLIVDE EHRFGVRHKE RIKAMRADVD ILTLTATPIP RTLNMAMSGM RDLSIIATPP
ARRLAVKTFV REYDNLVVRE AILREVLRGG QVYYLYNDVE NIQKAADRLA ELVPEARIAI
GHGQMREREL ERVMNDFHHQ RFNVLVCTTI IETGIDIPTA NTIIIERADH FGLAQLHQLR
GRVGRSHHQA YAWLLTPHPK AMTTDAQKRL EAIASLEDLG AGFALATHDL EIRGAGELLG
EDQSGSMETI GFSLYMELLE NAVDALKAGR EPSLEDLTSQ QTEVELRMPS LLPDDFIPDV
NTRLSFYKRI ASAKKENELE EIKVELIDRF GLLPDAARNL LDIARLRQQA QKLGIRKLEG
NEKGGVIEFA EKNHVNPMWL IGLLQKQPQH FRLDGPTRLK FTQELTERKT RMDWVRNFMR
QLEENAIA
//
