ID   W7QJY0_9ALTE            Unreviewed;       540 AA.
AC   W7QJY0;
DT   16-APR-2014, integrated into UniProtKB/TrEMBL.
DT   16-APR-2014, sequence version 1.
DT   27-MAR-2024, entry version 30.
DE   SubName: Full=Radical SAM domain/B12 binding domain-containing protein {ECO:0000313|EMBL:EWH08438.1};
GN   ORFNames=DS2_17262 {ECO:0000313|EMBL:EWH08438.1};
OS   Catenovulum agarivorans DS-2.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Alteromonadales;
OC   Alteromonadaceae; Catenovulum.
OX   NCBI_TaxID=1328313 {ECO:0000313|EMBL:EWH08438.1, ECO:0000313|Proteomes:UP000019276};
RN   [1] {ECO:0000313|EMBL:EWH08438.1, ECO:0000313|Proteomes:UP000019276}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DS-2 {ECO:0000313|EMBL:EWH08438.1,
RC   ECO:0000313|Proteomes:UP000019276};
RX   PubMed=24604650;
RA   Shan D., Li X., Gu Z., Wei G., Gao Z., Shao Z.;
RT   "Draft Genome Sequence of the Agar-Degrading Bacterium Catenovulum sp.
RT   Strain DS-2, Isolated from Intestines of Haliotis diversicolor.";
RL   Genome Announc. 2:e00144-14(2014).
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000256|ARBA:ARBA00001966};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:EWH08438.1}.
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DR   EMBL; ARZY01000046; EWH08438.1; -; Genomic_DNA.
DR   RefSeq; WP_035016209.1; NZ_ARZY01000046.1.
DR   AlphaFoldDB; W7QJY0; -.
DR   STRING; 1328313.DS2_17262; -.
DR   PATRIC; fig|1328313.3.peg.3528; -.
DR   eggNOG; COG1032; Bacteria.
DR   OrthoDB; 9801424at2; -.
DR   Proteomes; UP000019276; Unassembled WGS sequence.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR   GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   CDD; cd01335; Radical_SAM; 1.
DR   Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR   Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR   InterPro; IPR006158; Cobalamin-bd.
DR   InterPro; IPR036724; Cobalamin-bd_sf.
DR   InterPro; IPR025288; DUF4080.
DR   InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR   InterPro; IPR007197; rSAM.
DR   InterPro; IPR023404; rSAM_horseshoe.
DR   PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR   PANTHER; PTHR43409:SF18; RADICAL SAM SUPERFAMILY PROTEIN; 1.
DR   Pfam; PF02310; B12-binding; 1.
DR   Pfam; PF13311; DUF4080; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SMART; SM00729; Elp3; 1.
DR   SUPFAM; SSF52242; Cobalamin (vitamin B12)-binding domain; 1.
DR   SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR   PROSITE; PS51332; B12_BINDING; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   4: Predicted;
KW   Reference proteome {ECO:0000313|Proteomes:UP000019276}.
FT   DOMAIN          35..167
FT                   /note="B12-binding"
FT                   /evidence="ECO:0000259|PROSITE:PS51332"
FT   DOMAIN          192..416
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000259|PROSITE:PS51918"
FT   REGION          518..540
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        518..534
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   540 AA;  61864 MW;  E8F0C876DD958736 CRC64;
     MTEQAADSAM AISQINLADS KQASSLAKAN EPNQTKIILA TINAKYIHAS LGLRYLYANM
     QELQASCEIK EFVIQNRPID IVEQILQSKP RIVGFGVYIW NIVETTNVVS LLKVIAPEVK
     VVLGGPEVSY ETEQQAITQL ADYVLTGAAD LSFYRLCSDL LNDTPPEQKI LKSKPVELSE
     LQLPYQYYSD EDLKNRLLYV EASRGCPFKC EFCLSSLDTA SNPFELDTFL EQMDILYQRG
     ARNFKFIDRT FNLNINTSIK IMQFFLDRMT EDLYLHFEVV PDHLPRKLKE FLAQFPEDSL
     QFEVGIQTFE PQVQANISRK QNNNKSKDNL KWLRENTSAH IHADLIFGLP GETLESFRDS
     FNELYACNPQ EIQLGILKRL KGSPIIRHTD SFDLRFSPLP PFNILSTDRV DFATMQLVNR
     FARYWDMLGN SGRFKFTLPQ LLSGDPFDDF MMLTNWLYEK TGQTHQINLK RLFELLAQAT
     EALFPARYAT VLEALEQDYN ASKLKSVFAT LKLSAKEQPQ TNNQPAQAKL LQRQQRHKAS
//