ID W9GDD5_9MICO Unreviewed; 1011 AA.
AC W9GDD5;
DT 14-MAY-2014, integrated into UniProtKB/TrEMBL.
DT 14-MAY-2014, sequence version 1.
DT 27-MAR-2024, entry version 45.
DE RecName: Full=Multifunctional fusion protein {ECO:0000256|HAMAP-Rule:MF_00254, ECO:0000256|HAMAP-Rule:MF_00255};
DE Includes:
DE RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000256|HAMAP-Rule:MF_00255};
DE EC=6.1.1.14 {ECO:0000256|HAMAP-Rule:MF_00255};
DE AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000256|HAMAP-Rule:MF_00255};
DE Short=GlyRS {ECO:0000256|HAMAP-Rule:MF_00255};
DE Includes:
DE RecName: Full=Glycine--tRNA ligase alpha subunit {ECO:0000256|HAMAP-Rule:MF_00254};
DE AltName: Full=Glycyl-tRNA synthetase alpha subunit {ECO:0000256|HAMAP-Rule:MF_00254};
GN Name=glyQ {ECO:0000256|HAMAP-Rule:MF_00254};
GN Synonyms=glyS {ECO:0000256|HAMAP-Rule:MF_00255};
GN ORFNames=N864_15255 {ECO:0000313|EMBL:EWT04221.1};
OS Intrasporangium chromatireducens Q5-1.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Intrasporangiaceae;
OC Intrasporangium.
OX NCBI_TaxID=584657 {ECO:0000313|EMBL:EWT04221.1, ECO:0000313|Proteomes:UP000019494};
RN [1] {ECO:0000313|Proteomes:UP000019494}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Q5-1 {ECO:0000313|Proteomes:UP000019494};
RA Liu H., Wang G.;
RT "Intrasporangium oryzae NRRL B-24470.";
RL Submitted (AUG-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC EC=6.1.1.14; Evidence={ECO:0000256|ARBA:ARBA00000201,
CC ECO:0000256|HAMAP-Rule:MF_00255};
CC -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC {ECO:0000256|HAMAP-Rule:MF_00255}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00255}.
CC -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00008226, ECO:0000256|HAMAP-Rule:MF_00255}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EWT04221.1}.
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DR EMBL; AWQS01000298; EWT04221.1; -; Genomic_DNA.
DR AlphaFoldDB; W9GDD5; -.
DR PATRIC; fig|584657.3.peg.3904; -.
DR Proteomes; UP000019494; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR CDD; cd00733; GlyRS_alpha_core; 1.
DR HAMAP; MF_00254; Gly_tRNA_synth_alpha; 1.
DR HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR InterPro; IPR002310; Gly-tRNA_ligase_asu.
DR NCBIfam; TIGR00388; glyQ; 1.
DR NCBIfam; TIGR00211; glyS; 1.
DR PANTHER; PTHR30075:SF2; GLYCINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL 2; 1.
DR PANTHER; PTHR30075; GLYCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF02091; tRNA-synt_2e; 1.
DR Pfam; PF02092; tRNA_synt_2f; 1.
DR PRINTS; PR01044; TRNASYNTHGA.
DR SUPFAM; SSF55681; Class II aaRS and biotin synthetases; 1.
DR SUPFAM; SSF109604; HD-domain/PDEase-like; 1.
DR PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 2.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|HAMAP-Rule:MF_00255};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00255}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00255};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|HAMAP-Rule:MF_00255};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00255};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917, ECO:0000256|HAMAP-
KW Rule:MF_00255}; Reference proteome {ECO:0000313|Proteomes:UP000019494}.
SQ SEQUENCE 1011 AA; 110308 MW; 84880F55F77FED4C CRC64;
MTFQDALLTL QKFWTDRGCM VVQPYNTEVG AGTMNPATIL RVLGPEPWRV AYVEPSVRPD
DSRYGENPNR LQTHTQFQVI LKPDPGNPQE LYLESLEALG IDLTAHDVRF VEDNWAQPAI
GAWGLGWEVW LDGMEITQFT YFQQVGGQNL DPVAVELTYG MERIMMALQG KTHFKDMAYA
PGVSYGEVFA QSEYEMSRYY LDDADVTTNR ELFERYTAEA QRLVDARLPV PAHTYVLKSS
HAFNVLDARG AISTTERAKA FGTMRRLARE VSRLWVERRE ELGFPLLSSD LPPIAGRAVM
PPSRGAVEEY AAELGGLEDA EPRLLAVEIG VEELPPHVVE QAVEQVRSGL VEGLGASRLS
HGAVEVVGTP RRILALVPEV AAHEPDAEVM RKGPKVTAAF DADGRPTKAA EGFARGQKVD
VSQLVRAEFD GAEHVAVAVH QPGREVREVV GSVVADVITS LRADKNMRWG DPQLAYSRPI
RWVVALWGET VVPVRVSELG SGRTTYVDRR AAGPLVEIEN AAVLVPKLEE RGIVLDAAAR
RESVVAQAQS LAGAVDGIVD VEAEADLVDE ITNLVEAPRG ILGSFEERYL ELPEQILTTV
MRKHQRYLPV RSAEGRLMPH FITMANGDCD DALVRAGNEN VLRARYEDAA FFYDADLKVP
LEDLRAGIAK LTFEDRIGSM ADRADRIRDV AAALAELVAL DTTEGATLSR AGQLAKFDLA
SQMVVELSSL AGFMAREYAL KAGEAPEVAS ALYEMEQPRA AGDAVPQSRP GALLALADRF
DLLMAMFALG AKPTGSSDPF ALRRAALGVV AILRSQPTLA ALTVTQGLRA AADQLRQQGV
DVSDESIRTA TDFVTGRFAQ QLREEGVPTA LAAAVQPLAD APGQAEAALH DIESVRGREG
FPRLVEATQR ITRLVPQGSS AAYDPAVLRE EAERALHQKV TTLPDLSAAA LPEWANAAGP
VVPELERFFD EVLVMAEEPH LRAARLGLLQ TIVERAPRGI DWRALHSALS D
//
