ID W9T380_9PSED Unreviewed; 264 AA.
AC W9T380;
DT 14-MAY-2014, integrated into UniProtKB/TrEMBL.
DT 14-MAY-2014, sequence version 1.
DT 24-JAN-2024, entry version 38.
DE RecName: Full=2-keto-4-pentenoate hydratase {ECO:0000256|HAMAP-Rule:MF_01655};
DE EC=4.2.1.80 {ECO:0000256|HAMAP-Rule:MF_01655};
DE AltName: Full=2-hydroxypentadienoic acid hydratase {ECO:0000256|HAMAP-Rule:MF_01655};
GN Name=mhpD_1 {ECO:0000313|EMBL:EXF45017.1};
GN Synonyms=mhpD {ECO:0000256|HAMAP-Rule:MF_01655};
GN ORFNames=BAY1663_02525 {ECO:0000313|EMBL:EXF45017.1};
OS Pseudomonas sp. BAY1663.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=1439940 {ECO:0000313|EMBL:EXF45017.1, ECO:0000313|Proteomes:UP000019475};
RN [1] {ECO:0000313|EMBL:EXF45017.1, ECO:0000313|Proteomes:UP000019475}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BAY1663 {ECO:0000313|EMBL:EXF45017.1,
RC ECO:0000313|Proteomes:UP000019475};
RA Gyula P., Szabo Z., Robotka H., Bihari Z.;
RT "Genome sequencing of Pseudomonas sp. BAY1663.";
RL Submitted (DEC-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the conversion of 2-hydroxypentadienoic acid
CC (enolic form of 2-oxopent-4-enoate) to 4-hydroxy-2-ketopentanoic acid.
CC {ECO:0000256|HAMAP-Rule:MF_01655}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(S)-4-hydroxy-2-oxopentanoate = (2Z)-2-hydroxypenta-2,4-
CC dienoate + H2O; Xref=Rhea:RHEA:22580, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:67152, ChEBI:CHEBI:73143; EC=4.2.1.80;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01655};
CC -!- COFACTOR:
CC Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01655};
CC -!- PATHWAY: Aromatic compound metabolism; 3-phenylpropanoate degradation.
CC {ECO:0000256|HAMAP-Rule:MF_01655}.
CC -!- SIMILARITY: Belongs to the hydratase/decarboxylase family. MhpD
CC subfamily. {ECO:0000256|HAMAP-Rule:MF_01655}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EXF45017.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AZSV01000032; EXF45017.1; -; Genomic_DNA.
DR RefSeq; WP_037040649.1; NZ_AZSV01000032.1.
DR AlphaFoldDB; W9T380; -.
DR STRING; 1439940.BAY1663_02525; -.
DR PATRIC; fig|1439940.3.peg.2489; -.
DR eggNOG; COG3971; Bacteria.
DR OrthoDB; 9792137at2; -.
DR UniPathway; UPA00714; -.
DR Proteomes; UP000019475; Unassembled WGS sequence.
DR GO; GO:0008684; F:2-oxopent-4-enoate hydratase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR GO; GO:0019380; P:3-phenylpropionate catabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.90.850.10; Fumarylacetoacetase-like, C-terminal domain; 1.
DR HAMAP; MF_01655; MhpD; 1.
DR InterPro; IPR011234; Fumarylacetoacetase-like_C.
DR InterPro; IPR036663; Fumarylacetoacetase_C_sf.
DR InterPro; IPR023793; Keto_pentenoate-hydratase.
DR PANTHER; PTHR30143:SF0; 2-KETO-4-PENTENOATE HYDRATASE; 1.
DR PANTHER; PTHR30143; ACID HYDRATASE; 1.
DR Pfam; PF01557; FAA_hydrolase; 1.
DR SUPFAM; SSF56529; FAH; 1.
PE 3: Inferred from homology;
KW Aromatic hydrocarbons catabolism {ECO:0000256|ARBA:ARBA00022797,
KW ECO:0000256|HAMAP-Rule:MF_01655};
KW Lyase {ECO:0000256|HAMAP-Rule:MF_01655, ECO:0000313|EMBL:EXF45017.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000019475}.
FT DOMAIN 71..261
FT /note="Fumarylacetoacetase-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF01557"
SQ SEQUENCE 264 AA; 27777 MW; 27B0821D971E6C1E CRC64;
MSVTQDILEQ LAAALRAAEC SGEAITPLRE RIGEDAGEAA YAIQRLNVAH AEAAGRRVVG
RKIGLTNPKV QAQLGVDQPD FGTLFADMCY GDNETVPFGR VMQPKIEAEI ALILHRDLPR
ADTTFAELLE ATGWVLPSLE IVGSRVQGWN IRFVDTVADN ASSGCFVLGG PALPVAGVDL
RKASMSMTRN GELVCTGSGA ECLGHPLNAA VWLARTMARL GQPLRAGDIV LTGALGPMVG
VNPGDRFEAV IEGVGQVAAT FSAE
//
