ID WECG_ECOLI Reviewed; 246 AA.
AC P27836; Q2M8A3;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 15-JUL-1998, sequence version 2.
DT 24-JAN-2024, entry version 160.
DE RecName: Full=UDP-N-acetyl-D-mannosaminuronic acid transferase {ECO:0000255|HAMAP-Rule:MF_01001};
DE Short=UDP-ManNAcA transferase {ECO:0000255|HAMAP-Rule:MF_01001};
DE EC=2.4.1.180 {ECO:0000255|HAMAP-Rule:MF_01001};
GN Name=wecG {ECO:0000255|HAMAP-Rule:MF_01001};
GN Synonyms=rffM {ECO:0000255|HAMAP-Rule:MF_01001};
GN OrderedLocusNames=b3794, JW3770;
OS Escherichia coli (strain K12).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=1379743; DOI=10.1126/science.1379743;
RA Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.;
RT "Analysis of the Escherichia coli genome: DNA sequence of the region from
RT 84.5 to 86.5 minutes.";
RL Science 257:771-778(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION TO
RP 120-121.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP CATALYTIC ACTIVITY.
RX PubMed=3275612; DOI=10.1128/jb.170.1.228-233.1988;
RA Barr K., Ward S., Meier-Dieter U., Mayer H., Rick P.D.;
RT "Characterization of an Escherichia coli rff mutant defective in transfer
RT of N-acetylmannosaminuronic acid (ManNAcA) from UDP-ManNAcA to a lipid-
RT linked intermediate involved in enterobacterial common antigen synthesis.";
RL J. Bacteriol. 170:228-233(1988).
RN [5]
RP FUNCTION.
RX PubMed=1730666; DOI=10.1016/s0021-9258(18)48347-0;
RA Meier-Dieter U., Barr K., Starman R., Hatch L., Rick P.D.;
RT "Nucleotide sequence of the Escherichia coli rfe gene involved in the
RT synthesis of enterobacterial common antigen. Molecular cloning of the rfe-
RT rff gene cluster.";
RL J. Biol. Chem. 267:746-753(1992).
CC -!- FUNCTION: Catalyzes the synthesis of Und-PP-GlcNAc-ManNAcA (Lipid II),
CC the second lipid-linked intermediate involved in enterobacterial common
CC antigen (ECA) synthesis. {ECO:0000255|HAMAP-Rule:MF_01001,
CC ECO:0000269|PubMed:1730666}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=N-acetyl-alpha-D-glucosaminyl-di-trans,octa-cis-undecaprenyl
CC diphosphate + UDP-N-acetyl-alpha-D-mannosaminouronate = beta-D-
CC ManNAcA-(1->4)-alpha-D-GlcNAc-di-trans,octa-cis-undecaprenyl
CC diphosphate + H(+) + UDP; Xref=Rhea:RHEA:28366, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:61495, ChEBI:CHEBI:62959,
CC ChEBI:CHEBI:70731; EC=2.4.1.180; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01001, ECO:0000269|PubMed:3275612};
CC -!- PATHWAY: Bacterial outer membrane biogenesis; enterobacterial common
CC antigen biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01001}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 26 family.
CC {ECO:0000255|HAMAP-Rule:MF_01001}.
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DR EMBL; M87049; AAA67594.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76801.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77503.1; -; Genomic_DNA.
DR PIR; G65183; G65183.
DR RefSeq; NP_418242.1; NC_000913.3.
DR RefSeq; WP_001064040.1; NZ_SSZK01000025.1.
DR AlphaFoldDB; P27836; -.
DR SMR; P27836; -.
DR BioGRID; 4261340; 190.
DR BioGRID; 852599; 1.
DR DIP; DIP-11132N; -.
DR IntAct; P27836; 15.
DR STRING; 511145.b3794; -.
DR CAZy; GT26; Glycosyltransferase Family 26.
DR jPOST; P27836; -.
DR PaxDb; 511145-b3794; -.
DR DNASU; 948301; -.
DR EnsemblBacteria; AAC76801; AAC76801; b3794.
DR GeneID; 948301; -.
DR KEGG; ecj:JW3770; -.
DR KEGG; eco:b3794; -.
DR PATRIC; fig|1411691.4.peg.2911; -.
DR EchoBASE; EB1427; -.
DR eggNOG; COG1922; Bacteria.
DR HOGENOM; CLU_063203_3_2_6; -.
DR InParanoid; P27836; -.
DR OMA; LYQEPWR; -.
DR OrthoDB; 9808602at2; -.
DR PhylomeDB; P27836; -.
DR BioCyc; EcoCyc:UDPMANACATRANS-MONOMER; -.
DR BioCyc; MetaCyc:UDPMANACATRANS-MONOMER; -.
DR UniPathway; UPA00566; -.
DR PRO; PR:P27836; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016758; F:hexosyltransferase activity; IMP:EcoCyc.
DR GO; GO:0047241; F:lipopolysaccharide N-acetylmannosaminouronosyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0009246; P:enterobacterial common antigen biosynthetic process; IMP:EcoCyc.
DR CDD; cd06533; Glyco_transf_WecG_TagA; 1.
DR HAMAP; MF_01001; WecG_RffM; 1.
DR InterPro; IPR023085; UDP-ManNAcA_Trfase_WecG.
DR InterPro; IPR004629; WecG_TagA_CpsF.
DR NCBIfam; TIGR00696; wecG_tagA_cpsF; 1.
DR PANTHER; PTHR34136; -; 1.
DR PANTHER; PTHR34136:SF1; UDP-N-ACETYL-D-MANNOSAMINURONIC ACID TRANSFERASE; 1.
DR Pfam; PF03808; Glyco_tran_WecG; 1.
PE 1: Evidence at protein level;
KW Glycosyltransferase; Reference proteome; Transferase.
FT CHAIN 1..246
FT /note="UDP-N-acetyl-D-mannosaminuronic acid transferase"
FT /id="PRO_0000208426"
FT CONFLICT 120..121
FT /note="VL -> AV (in Ref. 1; AAA67594)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 246 AA; 27928 MW; 0D9435DEA6A69CAC CRC64;
MNNNTTAPTY TLRGLQLIGW RDMQHALDYL FADGQLKQGT LVAINAEKML TIEDNAEVRE
LINAAEFKYA DGISVVRSVR KKYPQAQVSR VAGADLWEEL MARAGKEGTP VFLVGGKPEV
LAQTEAKLRN QWNVNIVGSQ DGYFKPEQRQ ALFERIHASG AQIVTVAMGS PKQEIIMRDC
RLVHPDALYM GVGGTYDVFT GHVKRAPKIW QTLGLEWLYR LLSQPSRIKR QLRLLRYLRW
HYTGNL
//
