ID X4QF86_9PSED Unreviewed; 184 AA.
AC X4QF86;
DT 11-JUN-2014, integrated into UniProtKB/TrEMBL.
DT 11-JUN-2014, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE SubName: Full=Isoleucyl-tRNA synthetase {ECO:0000313|EMBL:AHU85781.1};
DE Flags: Fragment;
GN Name=ileS {ECO:0000313|EMBL:AHU85781.1};
OS Pseudomonas mandelii.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=75612 {ECO:0000313|EMBL:AHU85781.1};
RN [1] {ECO:0000313|EMBL:AHU85781.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=DSM 17967T {ECO:0000313|EMBL:AHU85781.1};
RA Andreani N.;
RL Submitted (MAR-2013) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:AHU85781.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=DSM 17967T {ECO:0000313|EMBL:AHU85781.1};
RX PubMed=24387861; DOI=10.1016/j.fm.2013.11.012;
RA Andreani N.A., Martino M.E., Fasolato L., Carraro L., Montemurro F.,
RA Mioni R., Bordin P., Cardazzo B.;
RT "Tracking the blue: a MLST approach to characterise the Pseudomonas
RT fluorescens group.";
RL Food Microbiol. 39:116-126(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-
CC isoleucyl-tRNA(Ile); Xref=Rhea:RHEA:11060, Rhea:RHEA-COMP:9666,
CC Rhea:RHEA-COMP:9695, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58045, ChEBI:CHEBI:78442, ChEBI:CHEBI:78528,
CC ChEBI:CHEBI:456215; EC=6.1.1.5;
CC Evidence={ECO:0000256|ARBA:ARBA00000114};
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC IleS type 1 subfamily. {ECO:0000256|ARBA:ARBA00006887}.
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DR EMBL; KC923521; AHU85781.1; -; Genomic_DNA.
DR AlphaFoldDB; X4QF86; -.
DR GO; GO:0004812; F:aminoacyl-tRNA ligase activity; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0006418; P:tRNA aminoacylation for protein translation; IEA:InterPro.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR PANTHER; PTHR42765:SF1; ISOLEUCINE--TRNA LIGASE, MITOCHONDRIAL; 1.
DR PANTHER; PTHR42765; SOLEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000313|EMBL:AHU85781.1}; ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917}.
FT DOMAIN 4..163
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:AHU85781.1"
FT NON_TER 184
FT /evidence="ECO:0000313|EMBL:AHU85781.1"
SQ SEQUENCE 184 AA; 20234 MW; 0FC528E5DCE5EC9D CRC64;
LREIGKDRPK FVLHDGPPYA NGTIHIGHAL NKILKDMIIR SKTLSGFDAP YVPGWDCHGL
PIEHKVEVTH GKNLGADKTR ELCRAYATEQ IEGQKSEFIR LGVLGDFANP YKTMDFKNEA
GEIRALAEIV KGGFVFKGLK PVNWCFDCGS ALAEAEVEYE NKKSSTIDVA FPIADEAKLA
AAFG
//
