ID ZDS_NOSS1 Reviewed; 479 AA.
AC Q9R6X4;
DT 02-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2002, sequence version 2.
DT 27-MAR-2024, entry version 118.
DE RecName: Full=Zeta-carotene desaturase;
DE EC=1.3.5.6;
DE AltName: Full=9,9'-di-cis-zeta-carotene desaturase;
DE AltName: Full=Carotene 7,8-desaturase;
GN Name=crtQ; OrderedLocusNames=all2382;
OS Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576).
OC Bacteria; Cyanobacteriota; Cyanophyceae; Nostocales; Nostocaceae; Nostoc.
OX NCBI_TaxID=103690;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Mann V., Hirschberg J.;
RT "Evolution of the carotenoid biosynthesis pathway: two pathways for
RT lycopene synthesis exist in Anabaena PCC7120.";
RL Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 7120 / SAG 25.82 / UTEX 2576;
RX PubMed=11759840; DOI=10.1093/dnares/8.5.205;
RA Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A.,
RA Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M.,
RA Takazawa M., Yamada M., Yasuda M., Tabata S.;
RT "Complete genomic sequence of the filamentous nitrogen-fixing
RT cyanobacterium Anabaena sp. strain PCC 7120.";
RL DNA Res. 8:205-213(2001).
CC -!- FUNCTION: Catalyzes the conversion of zeta-carotene to lycopene via the
CC intermediary of neurosporene. It carries out two consecutive
CC desaturations (introduction of double bonds) at positions C-7 and C-7'
CC (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=9,9'-di-cis-zeta-carotene + 2 a quinone = 7,7',9,9'-tetra-cis-
CC lycopene + 2 a quinol; Xref=Rhea:RHEA:30955, ChEBI:CHEBI:24646,
CC ChEBI:CHEBI:48716, ChEBI:CHEBI:62466, ChEBI:CHEBI:132124; EC=1.3.5.6;
CC -!- COFACTOR:
CC Name=NAD(+); Xref=ChEBI:CHEBI:57540; Evidence={ECO:0000305};
CC Name=NADP(+); Xref=ChEBI:CHEBI:58349; Evidence={ECO:0000305};
CC Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000305};
CC -!- PATHWAY: Carotenoid biosynthesis; lycopene biosynthesis.
CC -!- SIMILARITY: Belongs to the zeta carotene desaturase family.
CC {ECO:0000305}.
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DR EMBL; Y15115; CAB56041.1; -; Genomic_DNA.
DR EMBL; BA000019; BAB74081.1; -; Genomic_DNA.
DR PIR; AG2103; AG2103.
DR RefSeq; WP_010996538.1; NZ_RSCN01000002.1.
DR AlphaFoldDB; Q9R6X4; -.
DR SMR; Q9R6X4; -.
DR STRING; 103690.gene:10494412; -.
DR KEGG; ana:all2382; -.
DR eggNOG; COG3349; Bacteria.
DR OrthoDB; 438203at2; -.
DR BioCyc; MetaCyc:MONOMER-20345; -.
DR UniPathway; UPA00803; -.
DR Proteomes; UP000002483; Chromosome.
DR GO; GO:0052887; F:7,9,9'-tricis-neurosporene:quinone oxidoreductase activity; IEA:UniProtKB-EC.
DR GO; GO:0016719; F:9,9'-di-cis-zeta-carotene desaturase activity; IEA:UniProtKB-EC.
DR GO; GO:0052886; F:9,9'-dicis-carotene:quinone oxidoreductase activity; IEA:UniProtKB-EC.
DR GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 2.
DR InterPro; IPR002937; Amino_oxidase.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR014103; Zeta_caro_desat.
DR NCBIfam; TIGR02732; zeta_caro_desat; 1.
DR PANTHER; PTHR42923; PROTOPORPHYRINOGEN OXIDASE; 1.
DR PANTHER; PTHR42923:SF41; ZETA-CAROTENE DESATURASE, CHLOROPLASTIC_CHROMOPLASTIC; 1.
DR Pfam; PF01593; Amino_oxidase; 1.
DR PRINTS; PR00419; ADXRDTASE.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
PE 3: Inferred from homology;
KW Carotenoid biosynthesis; FAD; Flavoprotein; NAD; Oxidoreductase;
KW Reference proteome.
FT CHAIN 1..479
FT /note="Zeta-carotene desaturase"
FT /id="PRO_0000217856"
FT CONFLICT 92..94
FT /note="Missing (in Ref. 1)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 479 AA; 53579 MW; D8B14BD09B965F01 CRC64;
MRVAIVGAGL AGLATAIDLA DAGCEVQIFE SRPFVGGKVG SWIDGDGNHV EMGLHVFFGC
YYQLFELMNK VGAFSHLRLK EHTHTFVNKG GRTGALDFRF FTGAPFNGLK AFFTTSQLSL
QDKLQNAIAL GTSPIVRGLV DFEGAMKTIR NLDKISFADW FRSHGGSNGS IKRMWNPIAY
ALGFIDCENI SARCMLTIFQ FFAVRSEASV LRMLEGSPDE YLHQPILRYL EARGTKVYTR
RQVREIKYAE AEGQTRVTGI VVAKGDEVEE ITADAYVCAC DIPGIQRVLP QEWRKWSEFD
NIYKLDAVPV ATVQMRFDGW VTELQDENKR KQLKEAAGLD NLLYTADADF SCFADLALTS
PSDYYRQGQG SLLQLVLTPG DPFIKESNEA IAQHVLKQVY ELFPSSRELN MTWYSVVKLA
QSLYREAPGM DVYRPNQKTP IANFFLAGSY TQQDYIDSME GATVSGRRAA KVILDNIKK
//
