ID   O52545_AMYMD            Unreviewed;      5060 AA.
AC   O52545;
DT   01-JUN-1998, integrated into UniProtKB/TrEMBL.
DT   01-JUN-1998, sequence version 1.
DT   19-JAN-2010, entry version 60.
DE   SubName: Full=RifB;
GN   Name=rifB;
OS   Amycolatopsis mediterranei (Nocardia mediterranei).
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Pseudonocardineae; Pseudonocardiaceae; Amycolatopsis.
OX   NCBI_TaxID=33910;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=S699;
RX   MEDLINE=98174059; PubMed=9512878; DOI=10.1016/S1074-5521(98)90141-7;
RA   August P.R., Tang L., Yoon Y.J., Ning S., Muller R., Yu T.W.,
RA   Taylor M., Hoffmann D., Kim C.G., Zhang X., Hutchinson C.R.,
RA   Floss H.G.;
RT   "Biosynthesis of the ansamycin antibiotic rifamycin: deductions from
RT   the molecular analysis of the rif biosynthetic gene cluster of
RT   Amycolatopsis mediterranei S699.";
RL   Chem. Biol. 5:69-79(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=S699;
RX   MEDLINE=98165773; PubMed=9497318; DOI=10.1074/jbc.273.11.6030;
RA   Kim C.G., Yu T.W., Fryhle C.B., Handa S., Floss H.G.;
RT   "3-amino-5-hydroxybenzoic acid synthase, the terminal enzyme in the
RT   formation of the precursor of mC7N units in rifamycin and related
RT   antibiotics.";
RL   J. Biol. Chem. 273:6030-6040(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=S699;
RX   MEDLINE=21201076; PubMed=11278540; DOI=10.1074/jbc.M009667200;
RA   Yu T.W., Muller R., Muller M., Zhang X., Draeger G., Kim C.G.,
RA   Leistner E., Floss H.G.;
RT   "Mutational analysis and reconstituted expression of the biosynthetic
RT   genes involved in the formation of 3-amino-5-hydroxybenzoic acid, the
RT   starter unit of rifamycin biosynthesis in amycolatopsis Mediterranei
RT   S699.";
RL   J. Biol. Chem. 276:12546-12555(2001).
RN   [4]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=S699;
RA   Yu T.-W., Pogosova-Agadjanyan E.L., Kuan L.-Y., Bai L., Tin A.M.,
RA   Adman E., Floss H.G.;
RT   "Rifamycin insusceptibility: exploring the rif gene cluster of
RT   Amycolatopsis mediterranei S699.";
RL   Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=S699;
RA   Yu T., August P.R., Tang L., Yoon Y.J., Ning S., Mueller R.,
RA   Taylor M., Kim C., Zhang X., Pogosova-Agadjanyan E.L., Tin A.M.,
RA   Hutchinson C.R., Floss H.G.;
RL   Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
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DR   EMBL; AF040570; AAC01711.1; -; Genomic_DNA.
DR   HSSP; P25715; 1MLA.
DR   GO; GO:0000036; F:acyl carrier activity; IEA:InterPro.
DR   GO; GO:0048037; F:cofactor binding; IEA:InterPro.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR   GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009058; P:biosynthetic process; IEA:InterPro.
DR   InterPro; IPR001227; Ac_transferase_dom.
DR   InterPro; IPR009081; Acyl_carrier_prot-like.
DR   InterPro; IPR014043; Acyl_transferase.
DR   InterPro; IPR016035; Acyl_Trfase/lysoPlipase.
DR   InterPro; IPR000794; Beta-ketoacyl_synthase.
DR   InterPro; IPR002198; DH_sc/Rdtase_SDR.
DR   InterPro; IPR018201; Ketoacyl_synth_AS.
DR   InterPro; IPR014031; Ketoacyl_synth_C.
DR   InterPro; IPR014030; Ketoacyl_synth_N.
DR   InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR006163; Phsphopanteth_bd.
DR   InterPro; IPR020842; PKS/FAS_KR.
DR   InterPro; IPR020801; PKS_acyl_transferase.
DR   InterPro; IPR020841; PKS_Beta-ketoAc_synthase_reg.
DR   InterPro; IPR020807; PKS_dehydratase.
DR   InterPro; IPR013968; PKS_KR.
DR   InterPro; IPR020806; PKS_PP-bd.
DR   InterPro; IPR006162; PPantetheine_attach_site.
DR   InterPro; IPR016039; Thiolase-like.
DR   InterPro; IPR016038; Thiolase-like_subgr.
DR   Gene3D; G3DSA:3.40.366.10; Ac_transferase_reg; 3.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 3.
DR   Gene3D; G3DSA:3.40.47.10; Thiolase-like_subgr; 6.
DR   PANTHER; PTHR11712; Ketoacyl_synth; 1.
DR   Pfam; PF00698; Acyl_transf_1; 3.
DR   Pfam; PF00106; adh_short; 2.
DR   Pfam; PF00109; ketoacyl-synt; 3.
DR   Pfam; PF02801; Ketoacyl-synt_C; 3.
DR   Pfam; PF08659; KR; 1.
DR   Pfam; PF00550; PP-binding; 3.
DR   SMART; SM00827; PKS_AT; 3.
DR   SMART; SM00826; PKS_DH; 3.
DR   SMART; SM00822; PKS_KR; 3.
DR   SMART; SM00825; PKS_KS; 3.
DR   SMART; SM00823; PKS_PP; 3.
DR   PROSITE; PS50075; ACP_DOMAIN; 3.
DR   PROSITE; PS00606; B_KETOACYL_SYNTHASE; 2.
DR   PROSITE; PS00012; PHOSPHOPANTETHEINE; 3.
PE   4: Predicted;
KW   Phosphopantetheine; Transferase.
FT   CHAIN         1   1716       polyketide synthase module 4.
FT                                /FTId=PRO_5000053948.
FT   CHAIN        35    461       ketoacyl synthase.
FT                                /FTId=PRO_5000053949.
FT   CHAIN       564    881       methylmalonyl acyltransferase.
FT                                /FTId=PRO_5000053950.
FT   CHAIN       892   1091       dehydratase.
FT                                /FTId=PRO_5000053951.
FT   CHAIN      1346   1590       ketoreductase.
FT                                /FTId=PRO_5000053952.
FT   CHAIN      1624   1698       acyl carrier protein.
FT                                /FTId=PRO_5000053953.
FT   CHAIN      1717   3322       polyketide synthase module 5.
FT                                /FTId=PRO_5000053955.
FT   CHAIN      1717   2140       ketoacyl synthase.
FT                                /FTId=PRO_5000053954.
FT   CHAIN      2244   2552       methylmalonyl acyltransferase.
FT                                /FTId=PRO_5000053956.
FT   CHAIN      2562   2724       dehydratase.
FT                                /FTId=PRO_5000053957.
FT   CHAIN      2954   3198       ketoreductase.
FT                                /FTId=PRO_5000053958.
FT   CHAIN      3231   3305       acyl carrier protein.
FT                                /FTId=PRO_5000053959.
FT   CHAIN      3323   5060       polyketide synthase module 6.
FT                                /FTId=PRO_5000053961.
FT   CHAIN      3323   3748       ketoacyl synthase.
FT                                /FTId=PRO_5000053960.
FT   CHAIN      3850   4165       methylmalonyl acyltransferase.
FT                                /FTId=PRO_5000053962.
FT   CHAIN      4176   4375       dehydratase.
FT                                /FTId=PRO_5000053963.
FT   CHAIN      4657   4899       ketoreductase.
FT                                /FTId=PRO_5000053964.
FT   CHAIN      4932   5006       acyl carrier protein.
FT                                /FTId=PRO_5000053965.
SQ   SEQUENCE   5060 AA;  526557 MW;  13A6EEBE1A48A6B0 CRC64;
     MSAPNEQIVD ALRASLKENV RLQQENSALA AAAAEPVAIV SMACRYAGGI RGPEDFWRVV
     SEGADVYTGF PEDRGWDVEG LYHPDPDNPG TTYVREGAFL QDAAQFDAGF FGISPREALA
     MDPQQRQLLE VSWETLERAG IDPHSVRGSD IGVYAGVVHQ DYAPDLSGFE GFMSLERALG
     TAGGVASGRV AYTLGLEGPA VTVDTMCSSS LVAIHLAAQA LRRGECSMAL AGGSTVMATP
     GGFVGFARQR ALAFDGRCKS YAAAADGSGW AEGVGVLLLE RLSVARERGH QVLAVIRGSA
     VNQDGASNGL TAPNGPAQQR VIRKALASAG LTPSDVDTVE GHGTGTVLGD PIEVQALLAT
     YGQGRDPQQP LWLGSVKSVV GHTQAASGVA GVIKMVQSLR HGQLPATQHV DAPTPQVDWS
     AGAIELLAEG REWPRNGHPR RGGISSFGAS GTNAHMILEE APEDEPVTEA PAPTGVVPLV
     VSAATAASLA AQAGRLAEVG DVSLADVAGT LVSGRAMLSE RAVVVAGSHE EAVTGLRALA
     RGESAPGLLS GRGSGVPGKV VWVFPGQGTQ WAGMGRELLD SSEVFAARIA ECETALGRWV
     DWSLTDVLRG EADLLDRVDV VQPASFAVMV GLAAVWASLG VEPEAVVGHS QGEIAAACVS
     GALSLEDAAK VVALRSQAIA ASLAGRGGMA SVALSEEDAT ARLEPWAGRV EVAAVNGPTS
     VVIAGDAEAL DEALDALDDQ GVRIRRVAVD YASHTRHVEA ARDALAEMLG GIRAQAPEVP
     FYSTVTGGWV EDAGVLDGGY WYRNLRRQVR FGPAVAELIE QGHRVFVEVS AHPVLVQPIN
     ELVDDTEAVV TGTLRREDGG LRRLLASAAE LFVRGVTVDW SGVLPPSRRV ELPTYAFDHQ
     HYWLQMGGSA TDAVSLGLAG ADHPLLGAVV PLPQSDGLVF TSRLSLKSHP WLAGHAIGGV
     VLIPGTVYVD LALRAGDELG FGVLEELVIE APLVLGERGG VRVQVAVSGP NETGSRAVDV
     FSMREDGDEW TRHATGLLGA STSREPSRFD FAAWPPAGAE PIDVENFYTD LTERGYAYSG
     AFQGMRAVWR RGDEVFAEVA LPDDHREDAG KFGLHPALLD AALHTNAFAN PDDDRSVLPF
     AWNGLVLHAV GASALRVRVA PGGPDALTFQ AADETGGLVV TMDSLVSREV SAAQLETAAG
     EERDSLFQVD WIEVPATETA ATEHAEVLEA FGEAAPLELT SRVLEAVQSW LADAADEARL
     VVVTRGAVRE VTDPAGAAVW GLVRAAQAEN PGRIILVDTD GDVPLGAVLA SGEPQLAVRG
     NAFSVPRLAR ATGEVPEAPA VFSPEGTVLL TGGTGSLGGL VAKHLVARHG VRRLVLASRR
     GVAAEDLVTE LTEQGATVSV VACDVSDRDQ VAALLAEHRP TGIVHLAGLL DDGVIGALNR
     ERLAGVFAPK VDAVQHLDEL TRDLGLDAFV VFSSAAALMG SAGQGNYAAA NAFLDGLMAG
     RRAAGLPGVS LAWGLWEQAD GLTANLSATD QARMSRGGVL PMTPAEALDI FDIGLAAEQA
     LLVPIKLDLR TLRGQATAGG EVPHLLRGLV RASRRVTRTA AASGGGGLVH KLAGRPAEEQ
     EAVLLGIVQA EAAAVLGFNA PELAQGTRGF SDLGFDSLTA VELRNRLSAA TGVKLPATLV
     FDYPTPVALA RHLREELGET VAGAPATPVT TVADAGEPIA IVGMACRLPG GVMSPDDLWR
     MVAEGRDGMS PFPGDRGWDL DGLFDSDPER PGTAYIRQGG FLHEAALFDP GFFGISPREA
     LAMDPQQRLL LEASWEALER AGIDPTKARG DAVGVFSGVS IHDYLESLSN MPAELEGFVT
     TATAGSVASG RVSYTFGFEG PAVTVDTACS SSLVAIHLAA QALRQGECTM ALAGGVAVMG
     SPIGVIGMSR QRGMAEDGRV KAFADGADGT VLSEGVGIVV LERLSVARER GHRVLAVLRG
     SAVNQDGASN GLTAPNGPSQ QRVIRSALAG AGLQPSEVDV VEAHGTGTAL GDPIEAQALL
     ATYGKSRETP LWLGSLKSNI GHTQAAAGVA AVIKMVQALR HDTLPPTLHV QEPTKQVDWS
     AGAVELLTEG REWARNGHPR RAGVSSFGIS GTNAHLILEE APADDTAEAD VPDAVVPVVI
     SARSTGSLAG QAGRLAAFLD GDVPLTRVAG ALLSTRATLT DRAVVVAGSA EEARAGLTAL
     ARGESASGLV TGTAGMPGKT VWVFPGQGTQ WAGMGRELLE ASPVFAERIE ECAAALQPWI
     DWSLLDVLRG EGELDRVDVL QPACFAVMVG LAAVWASVGV VPDAVLGHSQ GEIAAACVSG
     ALSLEDAAKV VALRSQAIAA ELSGRGGMAS IQLSHDEVAA RLAPWAGRVE IAAVNGPASV
     VIAGDAEALT EAVEVLGGRR VAVDYASHTR HVEDIQDTLA ETLAGIDAQA PVVPFYSTVA
     GEWITDAGVV DGGYWYRNLR NQVGFGPAVA ELIEQGHGVF VEVSAHPVLV QPISELTDAV
     VTGTLRRDDG GVRRLLTSMA ELFVRGVPVD WATMAPPARV ELPTYAFDHQ HFWLSPPAVA
     DAPALGLAGA DHPLLGAVLP LPQSDGLVFT SRLSVRTHPW LADGVPAAAL VELAVRAGDE
     AGCPVLADLT VEKLLVLPES GGLRVQVIVS GERTVEVYSQ LEGAEDWIRN ATGHLSATAP
     AHEAFDFTAW PPAGAQQVDG LWRRGDEIFA EVALPEELDA GAFGIHPFLL DAAVQPVLAD
     DEQPAEWRSL VLHAAGASAL RVRLVPGGAL QAADETGGLV LTADSVAGRE LSAGKTRAGS
     LYRVDWTEVS IADSAVPANI EVVEAFGEEP LELTGRVLEA VQTWLVTAAD DARLVVVTRG
     AVREVTDPAG AAVWGLVRAA QAENPGRIFL IDTDGEIPAL TGDEPEIAVR GGKFFVPRIT
     RAEPSGAAVF RPDGTVLISG AGALGGLVAR RLVERHGVRK LVLASRRGRD ADGVADLVAD
     LAADVSVVAC DVSDRAQVAA LLDEHRPTAV VHTAGVIDAG VIETLDRDRL ATVFAPKVDA
     VRHLDELTRD RDLDAFVVYS SVSAVFMGAG SGSYAAANAF LDGLMANRRA AGLPGLSLAW
     GLWDQSTGMA AGTDEATRAR MSRRGGLQIM TQAEGMDLFD AALSSAESLL VPAKLDLRGV
     RADAAAGGVV PHMLRGLVRA GRAQARAAST VDNGLAGRLA GLAPADQLTL LLDLVRAQVA
     AVLGHADASA VRVDTAFKDA GFDSLTAVEL RNRMRTATGL KLPATLVFDY PNPQALARHL
     RDELGGAAQT PVTTAAAKAD LDEPIAIVGM ACRLPGGVAG PEDLWRLVAE GRDAVSSFPT
     DRGWDTDSLY DPDPARPGKT YTRHGGFLHE AGLFDAGFFG ISPREAVAMD PQQRLLLEAS
     WEAMEDAGVD PLSLKGNDVG VFTGMFGQGY VAPGDSVVTP ELEGFAGTGG SSSVASGRVS
     YVFGFEGPAV TIDSACSSSL VAMHLAAQSL RQGECSMALA GGATVMANPG AFVEFSRQRG
     LAVDGRCKAF AAAADGTGWA EGVGVVILER LSVARERGHR ILAVLRGSAV NQDGASNGLT
     APNGPSQQRV IRRALVSAGL APSDVDVVEA HGTGTTLGDP IEAQALLATY GKDRESPLWL
     GSLKSNIGHA QAAAGVAGVI KMVQALRHEV LPPTLHVDRP TPEVDWSAGA VELLTEAREW
     PRNGRPRRAG VSAFGVSGTN AHLILEEAPA EEPVPTPEVP LVPVVVSARS RASLAGQAGR
     LAGFVAGDAS LAGVARALVT NRAALTERAV MVVGSREEAV TNLEALARGE DPAAVVTGRA
     GSPGKLVWVF PGQGSQWIGM GRELLDSSPV FAERVAECAA ALEPWIDWSL LDVLRGESDL
     LDRVDVVQPA SFAMMVGLAA VWQSVGVRPD AVVGHSQGEI AAACVSGALS LQDAAKVVAL
     RSQAIATRLA GRGGMASVAL SEEDATAWLA PWADRVQVAA VNSPASVVIA GEAQALDEVV
     DALSGQEVRV RRVAVDYGSH TNQVEAIEDL LAETLAGIEA QAPKVPFYST LIGDWIRDAG
     IVDGGYWYRN LRNQVGFGPA VAELVRQGHG VFVEVSAHPV LVQPLSELSD DAVVTGSLRR
     EDGGLRRLLT SMAELYVQGV PLDWTAVLPR TGRVDLPKYA FDHRHYWLRP AESATDAASL
     GQGAADHPLL GAVVELPQSD GLVFTSRLSV RTHPWLADHA VGGVVILPGS GLAELAVRAG
     DEAGCTALDE LIIEAPLVVP AQGAVRVQVA LSGPDETGSR TVDLYSQRDG GAGTWTRHAT
     GVPSTAPAQE PEFDFHAWPP ADAERIDVET FYTDLAERGY GYGPAFQGLQ AVWRRDGDVF
     AEVALPEDLR KDAGRFGVHP ALLDAALQAA TAVGGDEPGQ PVLAFAWNGL VLHAAGASAL
     RVRLAPSGPD TLSVAAADET GGLVLTMESL VSRPVSAEQL GAAADAGHDA MFRVDWTELP
     AVPRAELPPW VRIDTADDVA ALAEKADAPP VVVWEAAGGD PALAVSSRVL EIMQAWLAAP
     AFEEARLVVT TRGAVPAGGD HTLTDPAAAA VWGLVRSAQA EHPDRVVLLD TDGEVPLGAV
     LASGEPQLAV RGTTFFVPRL ARATRLSDAP PAFDPDGTVL VSGAGSLGTL VARHLVTRHG
     VRRVVLASRQ GRDAEGAQDL ITELTGEGAD VSFVACDVSD RDQVAALLAG LPDLTGVVHT
     AGVFEDGVIE ALTPDQLANV YAAKVTAAMH LDELTRDRDL GAFVVFSSVA GVMGGGGQGP
     YAAANAFLDA AMASRQAAGL PGLSLAWGLW ERSSGMAAHL SEVDHARASR NGVLELTRAE
     GLALFDLGLR MAESLLVPIK LDLAAMRAST VPVLFRGLVR PSRTQARTAS TVDRGLAGRL
     AGLPVAERAA VLVDLVRGQV AVVLGYDGPE AVRPDTAFKD TGFDSLTSVE LRNRLREATG
     LKLPATLVFD YPNPLAVARY LGARLVPDGT ANGNGNGNGH SEDDRLRHAL AAIAAEDAGE
     ERSIADLGVD DLVQLAFGDE
//