ID   O52552_AMYMD            Unreviewed;       388 AA.
AC   O52552;
DT   01-JUN-1998, integrated into UniProtKB/TrEMBL.
DT   01-JUN-1998, sequence version 1.
DT   19-JAN-2010, entry version 40.
DE   SubName: Full=RifK;
GN   Name=rifK;
OS   Amycolatopsis mediterranei (Nocardia mediterranei).
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Pseudonocardineae; Pseudonocardiaceae; Amycolatopsis.
OX   NCBI_TaxID=33910;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=S699;
RX   MEDLINE=98174059; PubMed=9512878; DOI=10.1016/S1074-5521(98)90141-7;
RA   August P.R., Tang L., Yoon Y.J., Ning S., Muller R., Yu T.W.,
RA   Taylor M., Hoffmann D., Kim C.G., Zhang X., Hutchinson C.R.,
RA   Floss H.G.;
RT   "Biosynthesis of the ansamycin antibiotic rifamycin: deductions from
RT   the molecular analysis of the rif biosynthetic gene cluster of
RT   Amycolatopsis mediterranei S699.";
RL   Chem. Biol. 5:69-79(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=S699;
RX   MEDLINE=98165773; PubMed=9497318; DOI=10.1074/jbc.273.11.6030;
RA   Kim C.G., Yu T.W., Fryhle C.B., Handa S., Floss H.G.;
RT   "3-amino-5-hydroxybenzoic acid synthase, the terminal enzyme in the
RT   formation of the precursor of mC7N units in rifamycin and related
RT   antibiotics.";
RL   J. Biol. Chem. 273:6030-6040(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=S699;
RX   MEDLINE=21201076; PubMed=11278540; DOI=10.1074/jbc.M009667200;
RA   Yu T.W., Muller R., Muller M., Zhang X., Draeger G., Kim C.G.,
RA   Leistner E., Floss H.G.;
RT   "Mutational analysis and reconstituted expression of the biosynthetic
RT   genes involved in the formation of 3-amino-5-hydroxybenzoic acid, the
RT   starter unit of rifamycin biosynthesis in amycolatopsis Mediterranei
RT   S699.";
RL   J. Biol. Chem. 276:12546-12555(2001).
RN   [4]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=S699;
RA   Yu T.-W., Pogosova-Agadjanyan E.L., Kuan L.-Y., Bai L., Tin A.M.,
RA   Adman E., Floss H.G.;
RT   "Rifamycin insusceptibility: exploring the rif gene cluster of
RT   Amycolatopsis mediterranei S699.";
RL   Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=S699;
RA   Yu T., August P.R., Tang L., Yoon Y.J., Ning S., Mueller R.,
RA   Taylor M., Kim C., Zhang X., Pogosova-Agadjanyan E.L., Tin A.M.,
RA   Hutchinson C.R., Floss H.G.;
RL   Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
RX   MEDLINE=99364960; PubMed=10433690; DOI=10.1021/bi990018q;
RA   Eads J.C., Beeby M., Scapin G., Yu T.W., Floss H.G.;
RT   "Crystal structure of 3-amino-5-hydroxybenzoic acid (AHBA) synthase.";
RL   Biochemistry 38:9840-9849(1999).
CC   -!- SIMILARITY: Belongs to the degT/dnrJ/eryC1 family.
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DR   EMBL; AF040570; AAC01720.1; -; Genomic_DNA.
DR   PDB; 1B9H; X-ray; 2.00 A; A=1-388.
DR   PDB; 1B9I; X-ray; 2.00 A; A=5-388.
DR   PDBsum; 1B9H; -.
DR   PDBsum; 1B9I; -.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
DR   InterPro; IPR000653; DegT/StrS_aminotransferase.
DR   InterPro; IPR015424; PyrdxlP-dep_Trfase_major_dom.
DR   InterPro; IPR015421; PyrdxlP-dep_Trfase_major_sub1.
DR   InterPro; IPR015422; PyrdxlP-dep_Trfase_major_sub2.
DR   Gene3D; G3DSA:3.40.640.10; PyrdxlP-dep_Trfase_major_sub1; 1.
DR   Gene3D; G3DSA:3.90.1150.10; PyrdxlP-dep_Trfase_major_sub2; 1.
DR   Pfam; PF01041; DegT_DnrJ_EryC1; 1.
DR   PIRSF; PIRSF000390; PLP_StrS; 1.
PE   1: Evidence at protein level;
KW   Pyridoxal phosphate.
SQ   SEQUENCE   388 AA;  42138 MW;  6657B4F41F641190 CRC64;
     MNARKAPEFP AWPQYDDAER NGLVRALEQG QWWRMGGDEV NSFEREFAAH HGAAHALAVT
     NGTHALELAL QVMGVGPGTE VIVPAFTFIS SSQAAQRLGA VTVPVDVDAA TYNLDPEAVA
     AAVTPRTKVI MPVHMAGLMA DMDALAKISA DTGVPLLQDA AHAHGARWQG KRVGELDSIA
     TFSFQNGKLM TAGEGGAVVF PDGETEKYET AFLRHSCGRP RDDRRYFHKI AGSNMRLNEF
     SASVLRAQLA RLDEQIAVRD EPWTLLSRLL GAIDGVVPQG GDVRADRNSH YMAMFRIPGL
     TEERRNALVD RLVEAGLPAF AAFRAIYRTD AFWELGAPDE SVDAIARRCP NTDAISSDCV
     WLHHRVLLAG EPELHATAEI IADAVGRA
//