ID O52790_AMYMD Unreviewed; 1763 AA.
AC O52790; O52546;
DT 01-JUN-1998, integrated into UniProtKB/TrEMBL.
DT 01-JUN-1998, sequence version 1.
DT 19-JAN-2010, entry version 67.
DE SubName: Full=RifC;
DE SubName: Full=Rifamycin polyketide synthase, type 1;
GN Name=rifC;
OS Amycolatopsis mediterranei (Nocardia mediterranei).
OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC Pseudonocardineae; Pseudonocardiaceae; Amycolatopsis.
OX NCBI_TaxID=33910;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=LBG A3136;
RA Schupp T., Toupet C., Engel N., Goff S.;
RT "Cloning and sequence analysis of the putative rifamycin polyketide
RT synthase gene cluster from Amycolatopsis mediterranei.";
RL Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=S699;
RX MEDLINE=98174059; PubMed=9512878; DOI=10.1016/S1074-5521(98)90141-7;
RA August P.R., Tang L., Yoon Y.J., Ning S., Muller R., Yu T.W.,
RA Taylor M., Hoffmann D., Kim C.G., Zhang X., Hutchinson C.R.,
RA Floss H.G.;
RT "Biosynthesis of the ansamycin antibiotic rifamycin: deductions from
RT the molecular analysis of the rif biosynthetic gene cluster of
RT Amycolatopsis mediterranei S699.";
RL Chem. Biol. 5:69-79(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=S699;
RX MEDLINE=98165773; PubMed=9497318; DOI=10.1074/jbc.273.11.6030;
RA Kim C.G., Yu T.W., Fryhle C.B., Handa S., Floss H.G.;
RT "3-amino-5-hydroxybenzoic acid synthase, the terminal enzyme in the
RT formation of the precursor of mC7N units in rifamycin and related
RT antibiotics.";
RL J. Biol. Chem. 273:6030-6040(1998).
RN [4]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=S699;
RX MEDLINE=21201076; PubMed=11278540; DOI=10.1074/jbc.M009667200;
RA Yu T.W., Muller R., Muller M., Zhang X., Draeger G., Kim C.G.,
RA Leistner E., Floss H.G.;
RT "Mutational analysis and reconstituted expression of the biosynthetic
RT genes involved in the formation of 3-amino-5-hydroxybenzoic acid, the
RT starter unit of rifamycin biosynthesis in amycolatopsis Mediterranei
RT S699.";
RL J. Biol. Chem. 276:12546-12555(2001).
RN [5]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=S699;
RA Yu T.-W., Pogosova-Agadjanyan E.L., Kuan L.-Y., Bai L., Tin A.M.,
RA Adman E., Floss H.G.;
RT "Rifamycin insusceptibility: exploring the rif gene cluster of
RT Amycolatopsis mediterranei S699.";
RL Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=S699;
RA Yu T., August P.R., Tang L., Yoon Y.J., Ning S., Mueller R.,
RA Taylor M., Kim C., Zhang X., Pogosova-Agadjanyan E.L., Tin A.M.,
RA Hutchinson C.R., Floss H.G.;
RL Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
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DR EMBL; AF040570; AAC01712.2; -; Genomic_DNA.
DR EMBL; AJ223012; CAA11037.1; -; Genomic_DNA.
DR PIR; T17465; T17465.
DR HSSP; P72391; 1NM2.
DR SMR; O52790; 7-894, 1228-1564, 1322-1691.
DR GO; GO:0000036; F:acyl carrier activity; IEA:InterPro.
DR GO; GO:0048037; F:cofactor binding; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR GO; GO:0009058; P:biosynthetic process; IEA:InterPro.
DR InterPro; IPR001227; Ac_transferase_dom.
DR InterPro; IPR009081; Acyl_carrier_prot-like.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPlipase.
DR InterPro; IPR000794; Beta-ketoacyl_synthase.
DR InterPro; IPR002198; DH_sc/Rdtase_SDR.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR InterPro; IPR016040; NAD(P)-bd_dom.
DR InterPro; IPR006163; Phsphopanteth_bd.
DR InterPro; IPR020842; PKS/FAS_KR.
DR InterPro; IPR020801; PKS_acyl_transferase.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_reg.
DR InterPro; IPR020807; PKS_dehydratase.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR006162; PPantetheine_attach_site.
DR InterPro; IPR016039; Thiolase-like.
DR InterPro; IPR016038; Thiolase-like_subgr.
DR Gene3D; G3DSA:3.40.366.10; Ac_transferase_reg; 1.
DR Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR Gene3D; G3DSA:3.40.47.10; Thiolase-like_subgr; 2.
DR PANTHER; PTHR11712; Ketoacyl_synth; 1.
DR Pfam; PF00698; Acyl_transf_1; 1.
DR Pfam; PF00106; adh_short; 1.
DR Pfam; PF00109; ketoacyl-synt; 1.
DR Pfam; PF02801; Ketoacyl-synt_C; 1.
DR Pfam; PF00550; PP-binding; 1.
DR SMART; SM00827; PKS_AT; 1.
DR SMART; SM00826; PKS_DH; 1.
DR SMART; SM00822; PKS_KR; 1.
DR SMART; SM00825; PKS_KS; 1.
DR SMART; SM00823; PKS_PP; 1.
DR PROSITE; PS50075; ACP_DOMAIN; 1.
DR PROSITE; PS00606; B_KETOACYL_SYNTHASE; 1.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 1.
PE 4: Predicted;
KW Phosphopantetheine; Transferase.
SQ SEQUENCE 1763 AA; 182823 MW; DCD7FD776F006692 CRC64;
MVSASYEKVV EALRKSLEEV GTLKKRNRQL ADAAGEPIAI VGMACRLPGG VTGPGDLWRL
VAEGGDAVSG FPTDRCWDLD TLFDPDPDHA GTSYTDQGGF LHDAALFDPG FFGISPREAL
AMDPQQRLLL EASWEALEGV GLDPASLQGT DVGVFTGAGG SGYGGGLTGP EMQSFAGTGL
ASSVASGRVS YVFGFEGPAV TIDTACSSSL VAMHLAAQAL RQGDCSMALA GGAMVMSGPD
SFVVFSRQRG LATDGRCKAF ASGADGMVLA EGISVVVLER LSVARERGHR VLAVLRGSAV
NQDGASNGLT APNGPSQQRV IRAALANAGI GPSDVDLVEA HGTGTSLGDP IEAQALLATY
GQDRETPLWL GSLKSNIGHT QAAAGVASVI KVVQALRHGV MPPTLHVDEP SSQVDWSEGA
VELLTGSRDW PRGDRPRRAG VSSFGVSGTN VHLIIEEAPE EPAAAVPTSA DVVPLVVSAR
STGSLAGQAD RLTEVDVPLG HLAGALVAGR AVLEERAVVV AGSAEEARAG LGALARGEAA
PGVVTGTAGK PGKVVWVFPG QGTQWVGMGR ELLDASPVFA ERIKECAAAL DQWTDWSLLD
VLRGDGDLDS VEVLQPACFA VMVGLAAVWE SAGVRPDAVV GHSQGEIAAA CVSGALTLDD
AAKVVALRSQ AIAARLSGRG GMASVALSED EANARLGLWD GRIEVAAVNG PASVVIAGDA
QALDEALEVL AGDGVRVRQV AVDYASHTRH VEDIRDTLAE TLAGITAQAP DVPFRSTVTG
GWVRDADVLD GGYWYRNLRN QVRFGPAVAE LLEQGHGVFV EVSAHPVLVQ PISELTDAVV
TGTLRRDDGG LRRLLTSMAE LFVRGVRVDW ATLVPPARVD LPTYAFDHQH FWLRPAAQAD
AVSLGQAAAE HPLLGAVVRL PQSDGLVFTS RLSLRTHPWL ADHTIGGVVL FPGTGLVELA
VRAGDEAGCP VLDELVTEAP LVVPGQGGVN VQVTVSGPDQ NGLRTVDIHS QRDDVWTRHA
TGTVSATPAS SPGFDFTAWP PPDGQRVEIG DFYADLAERG YAYGPLFQGV RAVWQRGEDV
FAEVALPEDR REDAARFGLH PALLDAALQT GTIAAAASGQ PGKSVMPFSW NRLALHAVGA
AGLRVRVAPG GPDALTVEAA DETGAPVLTM DSLILREVAL DQLDTARAGS LYRVDWTPLP
TVDSAVPAGR AEVLEAFGEE PLDLTGRVLA ALQAWLSDAA EEARLVVVTR GAVPAGDGVV
SDPAGAAVWG LVRAAQAENP DRFVLLDTDG EVPLEAVLAT GEPQLALRGT TFSVPRLARV
TEPAEAPLTF RPDGTVLVSG AGTLGALAAR DLVTRHGVRR LVLASRRGRA AEGIDDLVAE
LTGHGAEVTV AACDVSDRDQ VAALLKEHAL TAVVHTAGVF DAGVTGALTR ERLAKVFAPK
VDAANHLDEL TRDLDLDAFI VYSSASSIFM GAGSGGYAAA NAYLDGLMAA RRAAGLPGLS
LAWGPWEQLT GMADTIDDLT LARMSRREGR GGVRALGSAD GMELFDAALA AGQALLVPIE
LDLREVRADA AGGGTVPHLL RGLVRAGRQA ARTAATEDGG LERRLAGLTV AEQEALLLDL
VRGQVAVVLG HADSSGVRAD AAFKDAGFDS LTSVELRNRL RETTGLKLPA TLVFDHPNPL
ALARHLRAEL AVDEASPADA VLAGLAGLEA AIAAAGAPDG DRITARLREL LKAAEAAEAR
PGTSGDLDTA SDEELFALVD GLD
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